Chinese Journal OF Rice Science >
Functional Analysis on Four Receptor-like Protein Kinases Under Salt Stress in Rice
Received date: 2020-09-05
Revised date: 2020-09-18
Online published: 2021-03-10
【Objective】Salinity stress is one of the main harsh environmental factor that greatly hinders rice growth and production, and it is meaningful to study salt-responsive genes for understanding the mechanisms behind plant response to salt stress and breeding salt tolerance variety. The receptor-like protein kinases are involved in regulation of plant cell signaling and responding to stress. In this study, we analyzed the expression pattern and function of four RLKs under salt stress. 【Method】 Quantitative real-time PCR was employed to detect the expression level of the four RLKs under NaCl treatment and its tissue specificity. 【Results】The transcription of Os04g0275100 was induced by NaCl, which was expressed mainly in roots. The transcription of Os07g0541900 was inhibited by NaCl, which was expressed mainly in roots. The transcription of Os09g0353200 was inhibited by NaCl, which was expressed mainly in leaves. The transcription of Os01g0852100 was inhibited by NaCl, which was expressed in roots, shoots, leaves and sheath. Loss-of-function mutants of the four RLKs were obtained by sequencing, which exhibited the identical response to NaCl as compared with the wild-type. 【Conclusion】The expression of the four RLKs identified in this study were regulated by NaCl and were tissue-specified. Mutation of one of the four RLKs has no effects on rice salt tolerance. This study lays a basis for revealing the biological function and mechanisms of RLKs under salt stress.
Key words： receptor-like protein kinase; salt stress; CRISPR/Cas9; japonica rice
Kai LU, Tao CHEN, Shu YAO, Wenhua LIANG, Xiaodong WEI, Yadong ZHANG, Cailin WANG . Functional Analysis on Four Receptor-like Protein Kinases Under Salt Stress in Rice[J]. Chinese Journal OF Rice Science, 2021 , 35(2) : 103 -111 . DOI: 10.16819/j.1001-7216.2021.0905
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