Chinese Journal OF Rice Science >
Physiological Characters and Gene Mapping of a Yellow Leaf and Early-senescence Mutant osyes1 in Rice
#These authors contributed equally to this work
Received date: 2020-02-05
Revised date: 2020-04-10
Online published: 2020-07-10
【Objective】Leaf is a main photosynthetic organ and its color mutants of rice are ideal materials for the study of photosynthesis, chlorophyll biosynthesis and metabolism, and chloroplast development in plants. 【Method】The mutant osyes1 (Oryza sativa yellow-leaf and early senescence 1) was obtained through 60Co γ-radiation treatment of indica cultivar Zixuan 1. The seedlings of osyes1 and its wild type were treated with exogenous H2O2. The SOD and CAT activities, ROS level, MDA content, soluble protein contents, chlorophyll contents, the net photosynthetic rate and chloroplast ultrastructure were analyzed for osyes1 and its wild type leaves at heading stage. The main agronomic traits of osyes1 and its wild type plants were analyzed under field conditions at maturation stage in Hangzhou. The recessive individuals in F2 population derived from osyes1/02428 were used to locate the gene by the map cloning method.【Results】The yellow-leaf and early-senescence symptoms started at 3- or 4-leaf stage, and gradually spread to all of the leaves after heading. Due to the early-senescence of the leaves of the mutant osyes1, its major agronomic traits including plant height, panicle length, grain number per panicle and the seed setting rate were markedly reduced. Moreover, the mutant osyes1 exhibited hyper-sensitivity to exogenous H2O2. The physiological analysis indicated that the contents of chlorophyll and the activities POD and CAT in the third-top leaf was significantly lower than those in the flag leaf and the second-top leaf in wild-type (WT) plants, but all of them in the mutant plants were significantly lower than those in its WT. Compared with the WT plants, the contents of MDA, H2O2 and O2- followed a steady increasing trend in the flag, second-top and third-top leaves of the mutant, while their soluble protein levels were progressively dropping. Genetic analysis confirmed that osyes1 was controlled by a single recessive nuclear gene, which was mapped to a region of 708 kb flanked by two SSR markers (RM21353 and RM21384) on the short arm of chromosome 7. 【Conclusion】In this work, the main agricultural traits were significantly reduced in osyes1 for the yellow leaf and early senescence. OsYES1 was located in a 708 kb range between RM21353 and RM21384 by map-based cloning strategy.
Key words： rice; osyes1; yellow and early-senescence; physiological analysis; gene mapping
Fudeng HUANG, Chaoyue ZHAO, Xin WU, Huanhuan HE, Fangmin CHENG, Chunshou LI, Gang PAN . Physiological Characters and Gene Mapping of a Yellow Leaf and Early-senescence Mutant osyes1 in Rice[J]. Chinese Journal OF Rice Science, 2020 , 34(4) : 307 -315 . DOI: 10.16819/j.1001-7216.2020.0301
| [1] | Okita T W, Sun J, Sakulringharoj C, Choi S B, Edwards G E, Kato C, Ito H, Matsui H.Increasing rice productivity and yield by manipulation of starch synthesis[J]. Novartis Foundation Symposium, 2001, 236: 135-146. |
| [2] | Julius B T, Leach K A, Tran T M, Mertz R A, Braun D M.Sugar transporters in plants: New insights and discoveries[J]. Plant and Cell Physiology, 2017, 58(9): 1442-1460. |
| [3] | 刘道宏. 植物叶片的衰老. 植物生理学通讯[J]. 1983(2): 14-19. |
| [3] | Liu D H.Plant leaf senescence.Plant Physiology Communications, 1983(2): 14-19. (in Chinese) |
| [4] | Thomas H, Smart C M.Crops that stay green[J]. Annals of Applied Biology, 1993, 123: 193-219. |
| [5] | 何冰, 刘玲珑, 张文伟, 万建民. 植物叶色突变体. 植物生理学通讯[J]. 2006, 42: 1-9 |
| [5] | HE B, Liu L L, Zhang W W, Wan J M.Plant leaf color mutants[J]. Plant Physiology Communications, 2006, 42: 1-9. (in Chinese) |
| [6] | Ougham H, Hörtensteiner S, Armstead I, Donnison I, King I, Thomas H, Mur L.The control of chlorophyll catabolism and the status of yellowing as a biomarker of leaf senescence[J]. Plant Biology (Stuttg), 2008(Suppl 1): 4-14. |
| [7] | 杜文凯,袁素霞,胡凤荣. 植物叶色突变分子机制的研究进展[J]. 分子植物育种, 2019, 17(6): 1888-1897. |
| [7] | Du W K, Yuan S X, Hu F R.Research progress on molecular mechanisms of the leaf color mutation[J]. Molecular Plant Breeding, 2019, 17(6): 1888-1897. (in Chinese with English abstract) |
| [8] | Peng Y L, Zou T, Li L M, Tang S W, Li Q, Zhang J, Chen Y J, Wang X C, Yang G T, Hu Y G.Map-based cloning and functional analysis of YE1 in rice, which is involved in light-dependent chlorophyll biogenesis and photoperiodic flowering pathway[J]. International Journal of Molecular Science, 2019, 20(3): 758. |
| [9] | Chen N G, Wang P R, Li C M, Wang Q, Pan J H, Xiao F L, Wang Y, Zhang K, Li C X, Yang B, Sun C H, Deng X J.A single nucleotide mutation of IspE gene participating in the MEP pathway for isoprenoid biosynthesis causes green-revertible yellow leaf phenotype in rice[J]. Plant and Cell Physiology, 2018, 59(9): 1905-1917. |
| [10] | Sheng Z H, Lü Y S, Li W, Luo R J, Wei X J, Xie L H, Jiao G L, Shao G N, Wang J L, Tang S Q, Hu P S.Yellow-Leaf 1 encodes a magnesium-protoporphyrin IX monomethyl ester cyclase, involved in chlorophyll biosynthesis in rice(Oryza sativa L.)[J/OL]. PLoS ONE, 2017, 12(5): e0177989. |
| [11] | Zhou Y, Gong Z Y, Yang Z F, Yuan Y, Zhu J Y, Wang M, Yuan F H, Wu S J, Wang Z Q, Yi C D, Xu T H, Ryom M, Gu M H, Liang G H.Mutation of the light-induced yellow leaf 1 gene, which encodes a geranylgeranyl reductase, affects chlorophyll biosynthesis and light sensitivity in rice[J/OL]. PLoS ONE, 2013, 8(9): e75299. |
| [12] | Murashige T, Skoog F.A revised medium for rapid growth and bio assays with tobacco tissue cultures[J]. Physiologia Plantarum, 1962, 15: 473-497. |
| [13] | Yang X, Gong P, Li K Y, Huang F D, Cheng F M, Pan G.A single cytosine deletion in the OsPLS1 gene encoding vacuolar-type H+-ATPase subunit A1 leads to premature leaf senescence and seed dormancy in rice[J]. Journal of Experimental Botany, 2016, 67(9): 2761-2776. |
| [14] | 龚盼, 黎坤瑜, 黄福灯, 韦荔全, 杨茜, 程方民, 潘刚. 水稻叶片早衰突变体ospls3的生理特征和基因定位[J]. 作物学报, 2016, 42(5): 667-674. |
| [14] | Gong P, Li K Y, Huang F D, Wei L Q, Yang X, Cheng F M, Pan G.Physiological characteristics and gene mapping of a precocious leaf senescence mutant ospls3 in rice[J]. Acta Agronomica Sinica, 2016, 42(5): 667-674. (in Chinese with English abstract) |
| [15] | Gong P, Luo Y M, Huang F D, Chen Y D, Zhao C Y, Wu X, Li K Y, Yang X, Cheng F M, Xiang X, Wu C Y, Pan G.Disruption of a Upf1-like helicase-encoding gene OsPLS2 triggers light-dependent premature leaf senescence in rice[J]. Plant Molecular Biology, 2019, 100(1-2): 133-149. |
| [16] | Pan G, Si P, Yu Q, Tu J M, Powles S.Non-target site mechanism of metribuzin tolerance in induced tolerant mutants of narrow-leafed lupin(Lupinus angustifolius L.)[J]. Crop and Pasture Science, 2012, 63(5): 452-458 |
| [17] | Shen Y J, Jiang H, Jin J P, Zhang Z B, Xi B, He Y Y, Wang G, Wang C, Qian L L, Li X, Yu Q B, Liu H J, Chen D H, Gao J H, Huang H, Shi T L, Yang Z N.Development of genome-wide DNA polymorphism database for map-based cloning of rice genes[J]. Plant Physiology, 2004, 135(3): 1198-1205. |
| [18] | Lim P O, Kim H J, Nam H G.Leaf senescence[J]. Annual Review of Plant Biology, 2007, 58: 115-136. |
| [19] | Miller G, Suzuki N, Ciftci-Yilmaz S, Mittler R.Reactive oxygen species homeostasis and signaling during drought and salinity stresses[J]. Plant Cell and Environment, 2010, 33(4): 453-467. |
| [20] | Jajic I, Sarna T, Strzalka K.Senescence, stress, and reactive oxygen species[J]. Plants(Basel), 2015, 4(3): 393-411. |
| [21] | Rogers H, Munné-Bosch S.Production and scavenging of reactive oxygen species and redox signaling during leaf and flower senescence: Similar but different[J]. Plant Physiology, 2016, 171(3): 1560-1568. |
| [22] | 华春, 王仁雷. 杂交稻及其三系叶片衰老过程中SOD、CAT活性和MDA含量的变化[J]. 西北植物学报, 2003, 23(3): 406-409. |
| [22] | Hua C, Wang R L.Changes of SOD and CAT activities and MDA content during senescence of hybrid rice and three lines leaves[J]. Acta Botanica Boreali-Occidentalia Sinica, 2003, 23(3): 406-409. (in Chinese with English abstract) |
| [23] | Wu X Y, Kuai B K, Jia J Z, Jing H C.Regulation of leaf senescence and crop genetic improvement[J]. Journal of Integrative Plant Biology, 2012, 54(12): 936-952. |
| [24] | Gregersen P L, Culetic A, Boschian L, Krupinska K.Plant senescence and crop productivity[J]. Plant Molecular Biology, 2013, 82(6): 603-622. |
| [25] | Woo H R, Kim H J, Lim P O, Nam H G.Leaf senescence: systems and dynamics aspects[J]. Annual Review of Plant Biology, 2019, 70: 347-376. |
| [26] | Bengoa Luoni S, Astigueta F H, Nicosia S, Moschen S, Fernandez P, Heinz R.Transcription factors associated with leaf senescence in crops[J]. Plants(Basel), 2019, 8(10): 411. |
| [27] | Kong Z S, Li M N, Yang W Q, Xu W Y, Xue Y B.A novel nuclear-localized CCCH-type zinc finger protein, OsDOS, is involved in delaying leaf senescence in rice[J]. Plant Physiology, 2006, 141(4): 1376-1388. |
| [28] | Jan A, Maruyama K, Todaka D, Kidokoro S, Abo M, Yoshimura E, Shinozaki K, Nakashima K, Yamaguchi-Shinozaki K.OsTZF1, a CCCH-tandem zinc finger protein, confers delayed senescence and stress tolerance in rice by regulating stress-related genes[J]. Plant Physiology, 2013, 161(3): 1202-1216. |
| [29] | Shim Y, Kang K, An G, Paek N C.Rice DNA-binding one zinc finger 24 (OsDOF24) delays leaf senescence in a jasmonate-mediated pathway[J]. Plant and Cell Physiology, 2019, 60(9): 2065-2076. |
| [30] | Han M, Kim C Y, Lee J, Lee S K, Jeon J S.OsWRKY42 represses OsMT1d and induces reactive oxygen species and leaf senescence in rice[J]. Molecules and Cells, 2014, 37(7): 532-539. |
| [31] | Na J K, Kim J K, Kim D Y, Assmann S M.Expression of potato RNA-binding proteins StUBA2a/b and StUBA2c induces hypersensitive-like cell death and early leaf senescence in Arabidopsis[J]. Journal of Experimental Botany, 2015, 66(13): 4023-4033. |
| [32] | Zhang B Y, Jia J H, Yang M, Yan C V, Han Y Z.Overexpression of a LAM domain containing RNA-binding protein LARP1c induces precocious leaf senescence in Arabidopsis[J]. Molecules and Cells, 2012, 34(4): 367-374. |
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