Chinese Journal OF Rice Science >
QTL Mapping for Heat Tolerance of Chalky Grain Rate of Oryza glaberrima Steud.
#These authors contributed equally to this work
Received date: 2019-07-26
Revised date: 2019-10-17
Online published: 2020-03-10
【Objective】The objective of the research is to identify a QTL for heat tolerance of chalky grain rate, so as to provide support for the appearance quality breeding and analysis of genetic mechanism of rice high temperature tolerance of chalky grain rate. 【Method】 We developed a chalky grain rate heat-tolerant CSIL(chromosomal segment introgression lines), CSIL05-2, by backcrossing and marker assisted selection with African cultivated rice acc. IRGC102309 (Oryza glaberrima Steud.) as donor parent and Asian cultivated rice R9311 (O. sativa L. subsp. indica Kato) as recipient parent. And a QTL for insensitity of heat tolerance of chalky grain rate on chromosome 5 was analyzed using secondary populations from CSIL05-2.【Results】In a BC6F2 segregation populations, the SSR marker RM1200 on chromosome 5 showed significant correlation with heat tolerance of chalky grain rate by single marker analysis（P=0.0005）. Using Cartographer 2.5 and the composite interval mapping with BC6F3 and BC6F4 populations, we further anchored the quantitative trait loci (QTL) associated with heat tolerance of chalky grain rate at filling stage to the same position within a 1.3 cM interval, which was designed as qHTCGR and explained 11.4% and 17.5% of the phenotypic variances, respectively. Using homozygous recombinants screened from BC6F4 populations, qHTCGR5 was also detected in a ~333.4 kb region between RM11633 and RM11642.【Conclusion】 The QTL controlling the heat tolerance of chalky grain rate qHTCGR5 is a novel QTL.
Key words： rice; chalky grain rate; heat tolerance; QTL; gene mapping
Zhibin CAO, Yao LI, Bohong ZENG, Linghua MAO, Yaohui CAI, Xiaofeng WU, Linfeng YUAN . QTL Mapping for Heat Tolerance of Chalky Grain Rate of Oryza glaberrima Steud.[J]. Chinese Journal OF Rice Science, 2020 , 34(2) : 135 -142 . DOI: 10.16819/j.1001-7216.2020.9086
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