Chinese Journal OF Rice Science >
Physiological and Molecular Mechanisms of Hydrogen Sulfide Enhancing Phosphorus Absorption and Transportation in Rice
Received date: 2019-05-13
Revised date: 2019-07-31
Online published: 2019-11-10
【Objective】 Low phosphorus (P) stress is one of the main factors limiting rice yield. Hydrogen sulfide (H2S) is produced in rice under flooding conditions. However, whether H2S is involved in the regulation of rice response to phosphorus deficiency stress as a signal molecule is still unclear. 【Method】 The content of H2S in rice was determined under normal and low P conditions to illuminate the role that H2S plays in regulating rice response to P deficiency stress. Rice was pretreated with 2 μmol/L H2S precursor NaHS for 1 day, then cultured for another 6 days under P sufficient and deficient conditions. The total P contents, acid phosphatase activity, antioxidant enzyme activity, xylem P concentration, P transporter genes’ expression and rice root architecture were determined to explore the physiological and molecular mechanism of H2S in regulating rice response to phosphorus deficiency stress. 【Conclusion】 Low P stress significantly increased the content of H2S in rice roots and shoots. NaHS pretreatment significantly increased total P contents, root acid phosphatase activity, root antioxidant enzymes activity, xylem P concentration and stimulated the expression of P transporter genes. Meanwhile, NaHS pretreatment modified rice root architecture, including increased total root length, total root surface area, total root volume and total root tip numbers. Therefore, our present study demonstrated that H2S improved P absorption and translocation in rice under P deficiency conditions and finally alleviate P deficiency stress in rice.
Key words： rice; P deficiency stress; H2S; P transporter; root architecture
Chunquan ZHU, Xiaochuang CAO, Lianfeng ZHU, Zhigang BAI, Jie HUANG, Qingduo LIANG, Qianyu JIN, Junhua ZHANG . Physiological and Molecular Mechanisms of Hydrogen Sulfide Enhancing Phosphorus Absorption and Transportation in Rice[J]. Chinese Journal OF Rice Science, 2019 , 33(6) : 532 -540 . DOI: 10.16819/j.1001-7216.2019.9055
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