Research Papers

Breeding Restore Lines of Hybrid Rice by Pyramiding Genes for Resistance to White Backed Planthoppers and Brown Planthoppers

Expand
  • 1Rice Research Institute, Fujian Academy of Agricultural Sciences/Research Bases of South-China, National Key Laboratory of Hybrid Rice of China/Key Laboratory of Hybrid Rice Germplasm Enhancement and Molecular Breeding in South China, Ministry of Agriculture and Rural Affairs, P. R. China/National Engineering Laboratory of Rice for China/ Fujian Engineering Laboratory of Crop Molecular Breeding/ Fujian Key Laboratory of Rice Molecular Breeding, Fuzhou 350003, China
    2College of Plant Protection, Fujian Agriculture and Forestry University/State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fuzhou 350002, China
*Corresponding author, E-mail: jianfzhang@163.com

Received date: 2019-01-10

  Revised date: 2019-03-18

  Online published: 2019-09-10

Abstract

【Objective】To breed restore lines of hybrid rice resistant to white backed planthoppers and brown planthoppers,【Method】we used B5 with brown planthopper resistance genes Bph14 and Bph15 and Fuhui 7011 with white backed planthoppers resistance gene qsI-4 as donor parents, and Fuhui 676, a main restorer line, as the recurrent parent through the molecular marker-assisted selection combined with field and indoor identification. The insect-resistant lines and their hybrid progenies were tested and their agronomic traits were analyzed. 【Result】Three restorer lines with three resistance genes to rice brown planthoppers and white backed planthoppers (Bph14, Bph15 and qsI-4) and three lines with two resistance genes were bred. Resistance identification indicated that the resistance of the restorer lines was moderately resistant or resistant. Two restorer lines with potential for production and application were screened out by examining the resistant strains and their crossing progenies and analyzing their agronomic traits.【Conclusion】This study provides new resistance germplasms against brown planthoppers and white backed planthoppers for hybrid rice breeding.

Cite this article

Yongsheng ZHU, Jianlin BAI, Hongguang XIE, Fangxi WU, Xi LUO, Shenfei JIANG, Wei HE, Liping CHEN, Qiuhua CAI, Huaan XIE, Jianfu ZHANG . Breeding Restore Lines of Hybrid Rice by Pyramiding Genes for Resistance to White Backed Planthoppers and Brown Planthoppers[J]. Chinese Journal OF Rice Science, 2019 , 33(5) : 421 -428 . DOI: 10.16819/j.1001-7216.2019.9007

References

[1] 沈君辉, 尚金梅, 刘光杰. 中国的白背飞虱研究概况. 中国水稻科学, 2003, 17(增刊): 7-22.
[1] Shen J H, Shang J M, Liu G J.Management of the whitebacked planthopper,Sogatella furcifera in China: A mini-review. Chin J Rice Sci, 2003, 17(Suppl): 7-22. (in Chinese with English abstract)
[2] 任西明, 向聪, 雷东阳, 管利凤. 水稻抗褐飞虱育种研究进展与展望. 作物研究, 2017, 31(4): 453-458.
[2] Ren X M, Xiang C, Lei D Y, Guan L F.Present status and prospect of resistance breeding of brow planthopper in rice.Crop Res, 2017, 31(4): 453-458.
[3] 蒋宁飞. 利用昌恢891和02428的回交自交系群体定位抗稻飞虱的QTL. 南昌: 江西农业大学, 2016.
[3] Jiang N F.Mapping quantitative trait loci(QTL) for rice planthopper resistance of Changhui 891/02428 backcross inbred lines. Nanchang: Jiangxi Agriculture University, 2016. (in Chinese with English abstract)
[4] 王彦华, 王鸣华. 褐飞虱抗药性及再猖獗研究进展. 农药, 2006, 45(4): 227-231.
[4] Wang Y H, Wang M H.Research progress onNilaparvata lugens insecticide resistance and proliferation of the resistant biotype. Agrochemicals, 2006, 45(4): 227-231. (in Chinese with English abstract)
[5] 姜辉, 林荣华, 刘亮, 瞿唯钢, 陶传江. 稻飞虱的危害及再猖獗机制. 昆虫知识, 2005, 42(6): 612-615.
[5] Jiang H, Lin R H, Liu L, Qu W G, Tao C J.Planthoppers damage to rice and the resurgence mechanism.Entomol Knowl, 2005, 42(6): 612-615. (in Chinese with English abstract)
[6] 侯丽媛, 于萍, 徐群, 袁筱萍, 余汉勇, 王一平, 王彩红, 万国, 彭锁堂, 魏兴华. 两个水稻抗褐飞虱隐性基因的遗传分析与初步定位. 中国水稻科学, 2010, 24(4): 367-371.
[6] Hou L Y, Yu P, Xu Q, Yuan Y P, Yu H Y, Wang Y P, Wang C H, Wan G, Peng S T, Wei X H.Genetic analysis and preliminary mapping of two recessive resistance genes in rice to brown planthopper,Nilaparvata lugens. Chin J Rice Sci, 2010, 24(4): 367-371. (in Chinese with English abstract)
[7] Jairin J, Sansen K, Wongboon W, Jate K.Detection of a brown planthopper resistance genebph4 at the same chromosomal position of Bph3 using two different genetic backgrounds of rice. Breeding Sci, 2010, 60(1): 71-75.
[8] Zhao Y, Huang J, Wang Z Z, Jing S L, Wang Y, Ouyang Y, Cai B D, Xin X F, Liu X, Zhang C X, Pan Y F, Ma R, Li Q F, Jiang W H, Zeng Y, Shangguan X X, Wang H Y, Du B, Zhu L L. Xu X, Feng Y Q, He S Y, Chen R Z, Zhang Q F, He G C.Allelic diversity in an NLR geneBPH9 enables rice to combat planthopper variation. Proc Natl Acad Sci, 2016, 113(45): 12850-12855.
[9] Du B, Zhang W, Liu B, Hu J, Wei Z, Shi Z He R, Zhu L, Chen R, Han B, He G. Identification and characterization ofBph14, a gene conferring resistance to brown planthopper in rice. Proc Natl Acad Sci, 2009, 106(52): 22163-22168.
[10] Lv W, Du B, Shangguan X, Zhao Y, Pan Y, Zhu L, He Y, He G.BAC and RNA sequencing reveal the brown planthopper resistance gene BPH15 in a recombination cold spot that mediates a unique defense mechanism.BMC Genom, 2014, 11(15): 674.
[11] Jena K K, Jeung J U, Lee J H, Choi C, Brar D S.High-resolution mapping of a new brown planthopper (BPH)resistance gene,Bph18(t), and marker-assisted selection for BPH resistance in rice, 2006, 112(2): 288-297.
[12] Tamura Y, Hattori M, Yoshioka H, Yoshioka M, Takahashi A, Wu J, Sentoku N, Yasui H.Map-based cloning and characterization of a brown planthopper resistance gene BPH26 fromOryza sativa L. ssp. indica cultivar ADR52. Sci Reps, 2014, 4: 58-72.
[13] Wang Y, Cao L, Zhang Y, Cao C, Liu F, Huang F, Qiu Y, Li R, Luo X.Map-based cloning and characterization of BPH29, a B3 domain-containing recessive gene conferring brown planthopper resistance in rice.J Exp Bot, 2015, 66(19): 6035-6045.
[14] Ren J, Gao F, Wu X, Lu X, Zeng L, Lv J, Su X, Luo H, Ren G.Bph32, a novel gene encoding an unknown SCR domain containing protein, confers resistance against the brown planthopper in rice. Sci Rep, 2016, 6: 37 645.
[15] Hu J, Zhou J, Peng X, Xu H, Liu C, Du B, Yuan H, Zhu L, He G.TheBphi008a gene interacts with the ethylene pathway and transcriptionally regulates MAPK genes in the response of rice to brown planthopper feeding. Plant Physiol, 2011, 156(2): 856-872.
[16] Guo J, Xu C, Wu D, Zhao Y, Qiu Y.Bph6 encodes an exocyst-localized protein and confers broad resistance to planthoppers in rice. Nat Genet, 2018, 50(2): 297-306.
[17] Huang Z, He Z, Shu L, Li X.Identification and mapping of two brown planthopper resistance genes in rice.Theor Appl Genet, 2001, 102: 929-934.
[18] 徐晓明, 程攀, 陈龙, 曲姗姗, 阴云火, 田发春, 彭炳生, 吴帅, 李土明, 周卫营. 应用分子标记辅助选育抗褐飞虱水稻两系不育系. 安徽农业科学, 2016, 44(20): 107-108.
[18] Xu X M, Cheng P, Chen L, Qu S S, Yin Y H, Tian F C, Peng B S, Wu S, Li T M, Zhou W Y.Breeding TGMS lines with resistance to brown planthopper by marker-assisted selection.J Anhui Agric Sci, 2016, 44(20): 107-108. (in Chinese with English abstract)
[19] 胡巍, 李艳芳, 胡侃, 江奕君, 张扬. 分子标记辅助选择抗褐飞虱基因改良桂农占的BPH抗性. 分子植物育种, 2015, 13(5): 951-960.
[19] Hu W, Li Y F, Hu K, Jiang Y J, Zhang Y.Improving BPH-resistance of rice cultivar Guinongzhan by marker-assisted selection for BPH-resistant genes.Mol Plant Breeding, 2015, 13(5): 951-960. (in Chinese with English abstract)
[20] 王海鹏, 黄晓西, 梁越洋, 朱军, 张翠霞, 王秀梅, 贡常委, 郑爱萍, 邓其明, 李双成, 王玲霞, 李平, 王世全. 转Cry30Fa1基因抗褐飞虱水稻的获得及鉴定. 中国水稻科学, 2016, 30(3): 256-264.
[20] Wang H P, Huang X X, Liang Y Y, Zhu J, Zhang C X, Wang X M, Gong C W, Zheng A P, Deng Q M, Li S C, Wang L X, Li P, Wang S Q.Development and Identification of insect resistant transgenic rice with Cry30Fa1 gene. Chin J Rice Sci, 2016, 30(3): 256-264. (in Chinese with English abstract)
[21] 张建福, 曾大力, 朱永生, 谢鸿光, 蔡秋华, 连玲, 吴方喜, 罗曦, 王颖姮, 郑轶, 谢华安. 分子标记辅助选择创制抗白背飞虱水稻恢复系. 中国水稻科学, 2013, 27(3): 329-334.
[21] Zhang J F, Zeng D L, Zhu Y S, Xie H G, Cai Q H, Lian L, Wu F X, Luo X, Wang Y H, Zheng Y, Xie H A.Breeding of rice restore lines with white-backed planthopper resistance by marker-assisted selection.Chin J Rice Sci, 2013, 27(3): 329-334. (in Chinese with English abstract)
[22] 刘光杰, 付志红, 沈君辉, 张亚辉. 水稻品种对稻飞虱抗性鉴定方法的比较研究. 中国水稻科学, 2002, 16(1): 52-56.
[22] Liu G J, Fu Z H, Shen J H, Zhang Y H.Comparative study on evaluation methods for resistance to rice planthoppers (Homoptera: Delphacidae) in rice.Chin J Rice Sci, 2002, 16(1): 52-56. (in Chinese with English abstract)
[23] 李荣华, 夏岩石, 刘顺枝, 孙莉丽, 郭培国, 廖绅裕, 陈健辉. 改进的CTAB提取植物DNA方法. 实验室研究与探索, 2009, 28(9): 14-16.
[23] Li R H, Xia Y S, Liu S Z, Sun L L, Guo P G, Liao S Y, Chen J H.CTAB improved method of DNA extraction in plant.Res Explor Lab, 2009, 28(9): 14-16. (in Chinese with English abstract)
[24] 何光存, 陈荣智, 杜波, 祝莉莉. 水稻抗褐飞虱基因Bph15的特异性共显性分子标记及其应用: CN107779522A.2018-03-09.
[24] He G C, Chen R Z, Du B, Zhu L L. Specific co-dominant molecular markers of rice brown planthopper resistance gene Bph15 and their application: CN107779522A.2018-03-09. (in Chinese)
[25] Sidhu G S, Khush G S.Genetic analysis of brown planthopper resistance in twenty varieties of rice,Oryza stativa L. Theor Appl Genet, 1978, 53: 199-203.
Outlines

/

Tel: 0571-63370278 E-mail: cjrs@263.net
Supported by Beijing Magtech Co., Ltd.