Chinese Journal OF Rice Science

Chinese Journal OF Rice Science >

2018 , Vol. 32 >Issue 6: 557 - 564

DOI: https://doi.org/10.16819/j.1001-7216.2018.8034

Orginal Article

Agronomic Traits of Marker-free Transgenic japonica Rice with Overexpression of OsPHF1 Under Low Phosphorus Environment

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  • 1 State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
    2 College of Material and Environmental Engineering, Hangzhou Dianzi University, Hangzhou 310018, China
    3 Laboratory of Photosynthesis and Environmental Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China
*Corresponding author, E-mail: steng@sibs.ac.cn

Received date: 2018-03-23

  Revised date: 2018-06-04

  Online published: 2018-11-10

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Abstract

【Objective】PHF1 (phosphate transporter assistance factor) regulates specific phosphorous transporters after transcriptional regulation and affects the utilization efficiency of phosphate. The main purpose is to develop selective-marker-free OsPHF1-overexpressed japonica rice (Kongyu 131), and study the agronomic traits of transgenic rice at different low phosphorus concentrations. It contributes to the cultivation of transgenic rice varieties with high phosphorus utilization efficiency.【Method】Marker-free OsPHF1-overexpressed transgenic lines were obtained by using double T-DNA methods, Agrobacterium-mediated infection and subsequent screening. The field experiments for T3 and T4 generations of transgenic plants were carried out under low phosphorus concentration (75 or 112.5 kg /hm2 calcium superphosphate) medium-to-low phosphorus concentrations (225 or 300 kg /hm2 calcium superphosphate) and normal phosphorus concentration (450 kg /hm2 calcium superphosphate).【Result】Three marker-free homozygous transgenic lines F18-18, F22-32 and F25-6 were obtained. The expression levels of OsPHF1 in lines F22-32 and F25-6 were much higher than that of wild type. Field experiments showed that compared with the wild type, the tiller number of F22-32 and F25-6 (T3 generation) were increased by 55% and 25%, respectively and the yield per plant of F22-32 and F25-6 were increased by 38%–34%, respectively under medium-to-low phosphorus environment (300 kg/hm2 calcium superphosphate). The yield of F22-32 and F25-6 transgenic lines (T4) increased by 30% to 35%, the biggest improvement under low phosphorus (112.5 kg /hm2 calcium superphosphate). Meantime, the tiller number and yield of F22-32 and F25-6 (T4) also showed obvious boost under medium-to-low phosphorus (225 kg /hm2 calcium superphosphate) levels. 【Conclusion】Marker-free OsPHF1 transgenic lines were cultivated using double T-DNA method. Two generation of homozygous transgenic lines showed significantly increased tiller number and yield at medium-to-low phosphorus level (112.5, 225 or 300 kg /hm2 calcium superphosphate).

Key words： OsPHF1; overexpression; marker-free transgenic rice; low phosphorus concentration; yield

Cite this article

Zhanghua HU, Xiuyan LIU, Yufeng WANG, Yu PENG, Xiaoliang SHI, Sheng TENG . Agronomic Traits of Marker-free Transgenic japonica Rice with Overexpression of OsPHF1 Under Low Phosphorus Environment[J]. Chinese Journal OF Rice Science, 2018 , 32(6) : 557 -564 . DOI: 10.16819/j.1001-7216.2018.8034

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