Chinese Journal OF Rice Science >
Genetic Analysis and Gene Mapping of an Albino Lethal Mutant in Rice
Received date: 2015-09-09
Revised date: 2015-12-05
Online published: 2016-03-10
An albino lethal mutant, temporarily termed as al14 (albino 14),was obtained from 60Co γ-ray radiated mutant pool of japonica rice variety Nipponbare. The mutant showed albino phenotype from germination, and died after 3-leaf stage. Genetic analysis revealed that the mutant phenotype was controlled by a single recessive nuclear gene. The chlorophyll and carotenoid contents in al14 declined dramatically. Transmission electron microscopy examination showed there were no obvious chloroplasts except prolamellar bodies in al14. The expression level of most genes of photosystemⅠand photosystem Ⅱdecreased dramatically, but the ribosomal genes and RNA polymerase genes in chloroplast increased notably in al14 mutant. In al14,the expression amount of PsbN, a photosystem Ⅱ protein N gene, was 118.23 times as high as that in wild type. By genetic mapping with an F2 population generated by crossing the heterozygous al14 plants with indica variety Huanghuazhan, the al14 locus was mapped within 40 kb on chromosome 6. So far there is no reported gene relative to leaf color, so it is a novel gene controlling chloroplast development.
Key words： rice; albino; gene mapping
Zhi-zhan CHU, Xiao-lin LIU, Yuan-ling CHEN, Yao-guang LIU . Genetic Analysis and Gene Mapping of an Albino Lethal Mutant in Rice[J]. Chinese Journal OF Rice Science, 2016 , 30(2) : 136 -142 . DOI: 10.16819/j.1001-7216.2016.5133
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