Chinese Journal OF Rice Science

Chinese Journal OF Rice Science >

2015 , Vol. 29 >Issue 2: 200 - 207

DOI: https://doi.org/10.3969/j.issn.1001-7216.2015.02.012

Orginal Article

Preliminary Study on Mechanisms of Growth Promotion in Rice Colonized by Piriformospora indica

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  • 1 State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China
    2China National Rice Research Institute, Hangzhou 310006, China
    3Analysis Center of Agrobiology and Environmental Sciences, Zhejiang University, Hangzhou 310058, China
*Corresponding author, E-mail:bglou@zju.edu.cn

Received date: 2014-05-06

  Revised date: 2014-06-20

  Online published: 2015-03-10

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Abstract

Piriformospora indica is a root endophytic fungus, which is able to establish beneficial symbiotic relationships with plant, and it can promote the growth of plants, enhance crop yield. In order to study the impact of P. indica on growth and development of rice, We analyzed the differences in plant height, biomass, tillering, heading, panicle properties, chlorophyll content, nitrate reductase (NR) activity, root activity and expression of growth-related genes between nonglutinous rice colonized and un-colonized by P. indica. The results showed that the plant height, biomass of shoot, the number of tillers, root activity and NR activity of rice colonized by P. indica were significantly higher than that in un-colonized ones. Rice colonized by P. indica headed 4-6 days earlier than the un-colonized rice. The contents of chlorophyll increased by 19.77% and 17.89% respectively after P. indica treatment for 3 and 4 weeks. RT-PCR analysis showed that the expression of auxin-regulated genes OsIAA13 and YUCCA were up-regulated in leaves of rice colonized by P. indica, being 1.49 and 1.30 folds as high as that of control plants. The expression of NRR, which negatively regulates the growth of rice, was 58% as high as that of control plants. The results indicated that the growth promotion of rice conferred by P. indica was relevant to chlorophyll content, NR activity, root activity and expression of growth-related genes. P. indica may promote the growth of rice through improving photosynthetic rate, enhancing mineral nutrition absorption and utilization and inducing auxin secretion.

Key words： Piriformospora indica; rice; chlorophyll; nitrate reductase; root activity; auxin

Cite this article

Jin-dan WU, Qian CHEN, Xiao-xi LIU, Fu-cheng LIN, Qi-kang GAO, Bing-gan LOU . Preliminary Study on Mechanisms of Growth Promotion in Rice Colonized by Piriformospora indica[J]. Chinese Journal OF Rice Science, 2015 , 29(2) : 200 -207 . DOI: 10.3969/j.issn.1001-7216.2015.02.012

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