Chinese Journal OF Rice Science >
The Response of Phytic Acid and Its Expression Profiles in Rice (Oryza sativa L.) Grain as Induced by Phosphorus Supply
Received date: 2014-08-05
Revised date: 2014-09-08
Online published: 2015-03-10
Phytic acid (PA) is the main storage form of phosphorus (P) in cereal grains. However, the relationship between external P supply and grain PA accumulation has not yet been fully understood. Two japonica rice cultivars were used to investigate the effects of phosphorus (P) treatments on grain phytic acid (PA) and its relation to inositol phosphate-related genes expression profiles using a culture of detached panicle of rice (Oryza sativa L.). Results showed that high P level caused a significant increase in grain PA, inorganic P (Pi) and total P contents. However, grain weight and PA content per grain were P concentration-dependent, and decreased under high P treatment (12P). High P supply decreased grain Zn and Fe concentrations, and their bio-availabilities. Moreover, P treatment also influenced all four PA related genes expression (RINO1, ITP5/6K-6, IPK2 and IPK1), especially for RINO1, a rate-limiting gene in PA synthesis and highly expressed in rice grains. Specifically, RION1 gene expression was up-regulated at optimum P (3P) concentration, but was significantly down-regulated under high P treatment (12P), which corresponded with the variation of grain PA content in rice, suggesting RION1 was a key metabolic gene in PA synthesis with P supply. In contrast, only a slight contribution of IPK2 gene was exhibited in PA synthesis induced by P supply.
Key words： phytic acid; detached rice panicle culture; RINO1; rice (Oryza sativa L.)
Da SU, Fu-biao WANG, Bing-ting LEI, Jue WANG, Gang PAN, Fang-min CHENG . The Response of Phytic Acid and Its Expression Profiles in Rice (Oryza sativa L.) Grain as Induced by Phosphorus Supply[J]. Chinese Journal OF Rice Science, 2015 , 29(2) : 159 -166 . DOI: 10.3969/j.issn.1001-7216.2015.02.007
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