Chinese Journal OF Rice Science >
Construction of a Vector for Large DNA Fragment Transformation in Magnaporthe oryzae
Received date: 2014-03-28
Revised date: 2014-06-11
Online published: 2015-01-10
It is an important means to study function of gene cluster using special vectors for large DNA fragment transformation. A vector was constructed for large DNA fragment transformation based on cosmid super Cos I vector and pBHt2 vector. More than 30 kb DNA fragment was transferred into the rice blast fungus successfully using Agrobacterium tumefaciens-mediated transformation. The results will promote functional analysis of gene cluster in Magnaporthe oryzae.
Key words： vector; transformation; large DNA fragment; Magnaporthe oryzae
Ya-ping CHEN, Wen-xiao SHI, Hong-kai WANG . Construction of a Vector for Large DNA Fragment Transformation in Magnaporthe oryzae[J]. Chinese Journal OF Rice Science, 2015 , 29(1) : 91 -96 . DOI: 10.3969/j.issn.1001-7216.2015.01.011
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