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  2004, Vol. 18 Issue (5): 455-460     DOI:
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Sensitivity Detection and Resistance Variation of Magnaporthe grisea to Kitazin P, Carbendazim and Tricyclazole
ZHANG Chuan-qing 1; ZHOU Ming-guo 1; SHAO Zhen-run 2; LIANG Gui-mei2
1Key Laboratory of Monitoring and Management of Plant Diseases and Insects; Ministry of Agriculture; Nanjing Agricultural University; Nanjing 210095; China; 2 National Agricultural Technical Extension and Service Center; Beijing 100026; China
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Abstract One hundred and twenty-nine Magnaporthe grisea isolates from Guangdong, Guangxi, Anhui and Jiangsu provinces of China in 2000 were tested for resistance frequency to kitazin P and carbendazim respectively at distinctive concentrations. The resistance frequency of M. grisea isolates to kitazin P, which had not been used in practice for about 10 years was as high as 79.1% and one carbendazim-resistant isolate was detected in Gaoyao City, Guangdong Province (with a frequency of 0.78%). Meanwhile, the minimum inhibition concentration (MIC) of hyphal melanization was adopted to detect the sensitivity of Magnaporthe grisea to the inhibitor, tricyclazole. The melanin biosynthesis in M. grisea showed several different sensitive degrees to tricyclazole, but no correlation between these MIC values completely inhibiting melanization in hyphal and the EC50 values of tricyclazole against rice blast tested in vivo was observed. Kitazin P-resistant mutants and carbendazim-resistant mutants were recovered by chemical taming and UV irradiation, but none tricyclazole-resistant mutant was got.
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ZHANG Chuan-qing
ZHOU Ming-guo
SHAO Zhen-run
LIANG Gui-mei
Key wordsMagnaporthe grisea   fungicide resistance   kitazin P   carbendazim   tricyclazole   mutant      
Received: 1900-01-01;
Cite this article:   
ZHANG Chuan-qing ,ZHOU Ming-guo ,SHAO Zhen-run et al. Sensitivity Detection and Resistance Variation of Magnaporthe grisea to Kitazin P, Carbendazim and Tricyclazole[J]. , 2004, 18(5): 455-460 .
 
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