Abstract: Rice stem-dipping method was used to monitor the susceptibility ofNilaparvata lugens(St°al) to buprofezin in 27 field populations,which were collected from eight provinces(region) in China from 1996 to 2007.The results showed that the populations from Jiangsu Province [Jiangpu(JPZY),Yizheng(YZZY) and Nantong(NTZY)],Anhui Province(Anqing,AQNS),and Guangxi [Nanning(GXNY) and Guilin(GLNS)] were very susceptible to buprofezin from 1996 to 2002,with resistance ratios of 0.3-to 3.5-fold.However,the populations of Jiangsu Province [Jiangpu(JPZY),2005] and Hainan Province [Lingshui(HNLS),2007] had developed a moderate level of resistance to buprofezin(28.8-and 19.4-fold,respectively);the populations from Guangxi [Nanning(GXNY) and Guilin(GLNS)],Hunan Province [Changde(CDTY)],Jiangxi Province [Xinjian(XJSM)],Zhejiang Province [Yuyao(YYLJ)],Anhui Province [Hexian(HXLY) and Qianshan(QSMC)],and Jiangsu Province [Nantong(NTZY)] showed a low level of resistance(5.6-to 9.1-fold) from 2005 to 2007;and the populations from Jiangxi Province [Shanggao(SGMS)],Zhejiang Province [Lanxi(LXYC)] and Hubei Province [Xiaogan(XGXC)] in 2006 were susceptible to buprofezin(2.5-to 4.1-fold).TheN.lugens collected from Anhui Province [Anqing(AQNS)] in 1996 had been selected with buprofezin for 13 generations in intermission during 22 generations,resulting in a LC50 decrease of 30%,and there was no significant difference in susceptibility between post-and pre-selections.However,after successive selection for 15 generations,the resistance ratio ascended to 1157.7-fold,which showed thatN.lugens had the risk of developing a high level of resistance to buprofezin if this insecticide was applied successively and alone.The strategy of resistance management of N.lugens was also proposed.

Key words: Nilaparvata lugens, buprofezin, resistance monitoring, resistance selection, realized heritability

摘要： 1996-2007年间采用稻茎浸渍法测定了我国8省(区)27个褐飞虱种群对噻嗪酮的敏感性。 在1996-2002年间， 江苏\[江浦(JPZY)、仪征(YZZY)和南通(NTZY)\]、安徽\[安庆(AQNS)\]、广西\[南宁(GXNY)和桂林(GLNS)\]褐飞虱种群对噻嗪酮均为敏感至敏感性下降 (抗性倍数为0.3~3.5倍)。 在2005-2007年间，监测到江苏 \[江浦(JPZY), 2005年\]和海南\[陵水(HNLS)，2007年\]褐飞虱种群对噻嗪酮达中等水平抗性(抗性倍数分别为28.8倍和19.4倍)；其余种群如广西\[南宁(GXNY)和桂林(GLNS)\]、湖南\[常德(CDTY)\]、浙江\[余姚(YYLJ)\]、江西\[新建(XJSM)\]、安徽\[和县(HXLY)、潜山(QSMC)\]及江苏\[南通(NTZY)\]等地褐飞虱种群对噻嗪酮为低水平抗性(抗性倍数为5.6~9.1倍)；而江西\[上高(SGMS)\]、浙江\[兰溪(LXYC)\]和湖北\[孝感(XGXC)\]3个褐飞虱种群对噻嗪酮仍为敏感至敏感性下降(抗性倍数2.5~4.1倍)。室内饲养22代，用噻嗪酮对1996年采自安徽\[安庆(AQNS)\]的褐飞虱间断筛选13代，它的LC50下降了30%，筛选前后抗性水平没有明显差异；随后连续筛选15代，抗性倍数达1157.7倍。上述结果表明，褐飞虱对噻嗪酮已产生低到中等水平抗性，如果连续单一使用噻嗪酮，褐飞虱将具有产生高水平抗性的风险。此外，还对褐飞虱的抗性治理进行了讨论。

关键词: 褐飞虱, 噻嗪酮, 抗性监测, 抗性筛选, 现实遗传力