Abstract: A survey on isolation and detection of the casual organism of bacterial grain rot from rice was conducted during 1997-2006. In 2006, six pathogenic bacterial strains were isolated from two asymptomatic rice seed samples (Oryza sativa L.) originally produced in Hainan Province and then planted in Zhejiang Province. They were identified as Burkholderia glumae, which is the causal organism of bacterial grain rot of rice, by physiological characteristics, colony morphology, pathogenicity test, Biolog, fatty acid methyl ester (FAME) analysis and RAPDPCR compared with the four standard reference strains. It is confirmed that there exsits Burkholderia glumae in socalled “health looking seeds”.

Key words: Burkholderia glumae, phenotypical identification, molecular identification, pathogenicity

摘要： 经多年的病原菌分离监测, 于2006年从2份浙江省稻种(来自海南的健康籼稻)上分离到6株病原细菌，经25项主要生理生化特性、菌落形态、致病性、Biolog、脂肪酸分析和RAPDPCR鉴定，发现这种病原细菌为Burkholderia glumae， 是引起水稻细菌性谷枯病的病原菌。此外，实验证明“健康稻种”也可能存在水稻细菌性谷枯病菌。

关键词: 水稻细菌性谷枯病菌, 表型鉴定, 分子鉴定