Systematic Identification of Rice ABC1 Genes and Their Expression Analysis under Abiotic Stresses
GAO Qing-song;ZHANG Dan; XU Liang; XU Chen-wu*
Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, China; *Corresponding author, E-mail: firstname.lastname@example.org
Abstract ABC1 (Activity of bc1 complex) family belongs to protein kinase families, whose members widely exist in prokaryotes and eukaryotes. It has been reported that several plant ABC1 genes participate in abiotic stress response. To understand the structure and function of ABC1 genes in rice, the systematic characterization of rice and Arabidopsis ABC1 genes and the expression analysis of rice ABC1 genes were performed. A total of 15 and 17 members of rice and Arabidopsis ABC1 families were identified by the bioinformatics method. The phylogenetic analysis of these proteins suggested that divergence of this family had occurred and the main characteristics had established before the dicotmonocot split; speciesspecific expansion contributed to the evolution of this family in rice and Arabidopsis after the split of monocots and dicots. Intron/exon stucture analysis indicated that most of the orthologous genes had similar exon sizes but diverse intron sizes and the rice genes contained more large introns; intron gain was an important event accompanying the recent evolution of rice ABC1 family. Multiple sequence alignment revealed one conserved amino acid segment and four conserved amino acids of the ABC1 domain. Online subcellular localization predicted that nine rice ABC1 proteins were localized in the chloroplast. Realtime RTPCR assay established that rice ABC1 genes expressed primarily in leaves and could be modulated by a broad range of abiotic factors such as H2O2, abscisic acid, low temperature, drought, darkness and high salinity. These results illustrate that rice ABC1 family played roles not only in the environmental stress response but also likely in the specific biological process of rice.
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