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  2010, Vol. 24 Issue (6): 601-607     DOI: 10.3969/j.issn.1001-7216.2010.06.007
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Estimation of Mating System in Natural Oryza rufipogon Populations by SSR Markers
LI Xiao-xiang1,2; LIU Yong2, *;DUAN Yong-hong1 ; WANG Shu-hong1 ;ZHAN Qing-cai1 ;SUN Gui-hua1 ; GAO Li-zhi3, *
1Rice Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China; 2Graduate School of Central South University, Changsha 410083, China; 3Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China; *Corresponding author, E-mail:
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Abstract  Oryza rufipogon the most important germplasm for rice improvement.Better understanding its mating system will provide useful information for further in situ conservation and management of O. rufipogon. By using SSR method,the genetic diversity and outcrossing rate of six natural O.rufipogon populations were estimated. The levels of genetic diversity varied among the populations, with the Nei′s diversity indices ranged from 0.3166 to 0.6075. There was a significant genetic differentiation (Fst=0.422) among the sampled populations. Two populations exhibited a heterozygosity excess, whereas the other four populations had a heterozygosity deficit. The O.rufipogon populations possessed a mixed type of mating system, although the outcrossing rate was variable among the populations. The multilocus outcrossing rate ranged from 15.1% to 418%,and the singlelocus outcrossing rate from 10.1% to 28.5%.There were significant differences between the multilocus outcrossing rate and the singlelocus outcrossing rate, and high values of correlation of paternity in the populations, indicating a considerable inbreeding existed in the populations. The correlation of paternity for the multilocus significantly differed from the correlation of paternity for the singlelocus, suggesting that there was a substructure occurred in each sampled population. Some measures for further improving insitu and exsitu conservation of O. rufipogon were also discussed.
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Articles by authors
LI Xiao-xiang
LIU Yong
DUAN Yong-hong
WANG Shu-hong
ZHAN Qing-cai
SUN Gui-hua
GAO Li-zhi
Key wordsOryza rufipogon   mating system   in-situ conservation   ex-situ conservation   genetic diversity   simple sequence repeats     
Received: 1900-01-01;
Cite this article:   
LI Xiao-xiang,LIU Yong,DUAN Yong-hong et al. Estimation of Mating System in Natural Oryza rufipogon Populations by SSR Markers[J]. , 2010, 24(6): 601-607 .
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