中国水稻科学
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  2010, Vol. 24 Issue (6): 601-607     DOI: 10.3969/j.issn.1001-7216.2010.06.007
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Estimation of Mating System in Natural Oryza rufipogon Populations by SSR Markers
LI Xiao-xiang1,2; LIU Yong2, *;DUAN Yong-hong1 ; WANG Shu-hong1 ;ZHAN Qing-cai1 ;SUN Gui-hua1 ; GAO Li-zhi3, *
1Rice Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China; 2Graduate School of Central South University, Changsha 410083, China; 3Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China; *Corresponding author, E-mail: haoasliu@163.com
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Abstract  Oryza rufipogon Griff.is the most important germplasm for rice improvement.Better understanding its mating system will provide useful information for further in situ conservation and management of O. rufipogon. By using SSR method,the genetic diversity and outcrossing rate of six natural O.rufipogon populations were estimated. The levels of genetic diversity varied among the populations, with the Nei′s diversity indices ranged from 0.3166 to 0.6075. There was a significant genetic differentiation (Fst=0.422) among the sampled populations. Two populations exhibited a heterozygosity excess, whereas the other four populations had a heterozygosity deficit. The O.rufipogon populations possessed a mixed type of mating system, although the outcrossing rate was variable among the populations. The multilocus outcrossing rate ranged from 15.1% to 418%,and the singlelocus outcrossing rate from 10.1% to 28.5%.There were significant differences between the multilocus outcrossing rate and the singlelocus outcrossing rate, and high values of correlation of paternity in the populations, indicating a considerable inbreeding existed in the populations. The correlation of paternity for the multilocus significantly differed from the correlation of paternity for the singlelocus, suggesting that there was a substructure occurred in each sampled population. Some measures for further improving insitu and exsitu conservation of O. rufipogon were also discussed.
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LI Xiao-xiang
LIU Yong
DUAN Yong-hong
WANG Shu-hong
ZHAN Qing-cai
SUN Gui-hua
GAO Li-zhi
Key wordsOryza rufipogon   mating system   in-situ conservation   ex-situ conservation   genetic diversity   simple sequence repeats     
Received: 1900-01-01;
Cite this article:   
LI Xiao-xiang,LIU Yong,DUAN Yong-hong et al. Estimation of Mating System in Natural Oryza rufipogon Populations by SSR Markers[J]. , 2010, 24(6): 601-607 .
 
[1] 张大勇, 姜新华. 植物交配系统的进化、资源分配对策与遗传多样性. 植物生态学报, 2001, 25(2): 130-143.
[2] 何田华, 葛颂. 植物种群交配系统、亲本分析以及基因流动研究. 植物生态学报, 2001, 25(2): 144-154.
[3] 叶平扬, 董姗姗, 卢宝荣, 等. 普通野生稻小种群的交配系统与遗传多样性. 生态学报, 2008, 28(4): 1608-1615.
[4] Oka H I, Morishima H. Variation in the breeding systems of a wild rice, Oryza perennis. Evolution, 1967, 21: 249-258.
[5] Morishima H, Barbier P. Mating system and genetic structure of natural populations in wild rice, Oryzae rufipogon. Plant Species Biol, 1990, 5: 31-39.
[6] Xu X, Lu B R, Yoland A H, et al. Inferring population history from fine-scale spatial genetic analysis in Oryza rufipogon(Poaceae). Mol Ecol, 2006, 15: 1535-1544.
[7] McCouch S R, Teytelman L, Xu Y B, et al. Development and mapping of 2240 new SSR markers for rice(Oryza sativa L.). DNA Res, 2002, 9: 199-207.
[8] Song Z P, Xu X, Wang B, et al. Genetic diversity in the northernmost Oryza rufipogon populations estimated by SSR markers.Theor Appl Genet, 2003, 107: 1492-1499.
[9] Rogers O S, Bendich A J. Extraction of DNA from plant tissues//Gelvin S B, Schiperoort R A, Verma D P S. Plant Molecular Biology Manual A6. Dordrecht: Kluwer Academic Publisher, 1998: 1-10.
[10] Klimyuk V I, Carroll B J, Thomas C M, et al. Alkali treatment for rapid preparation of plant material for reliable PCR analysis. Plant J, 1993, 3: 493-494.
[11] 詹庆才. 利用微卫星DNA标记进行杂交水稻种子纯度鉴定的研究. 杂交水稻, 2002, 17(5): 46-50.
[12] 桑贤春, 何光华, 张毅, 等. 水稻PCR扩增模板的快速制备. 遗传, 2003, 25(6): 705-707.
[13] Yeh F C, Yang R. Microsoft Window-based freeware for population genetic analysis (POPGENE). Version 1.31. Edmonton: Unversity of Alberta, 1999.
[14] Nei M. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 1978, 89: 583-590.
[15] Weir B S, Cockerham C C. Estimating F-statistics for the analysis of population structure. Evolution, 1984, 38: 1358-1370.
[16] Ritland K. Extensions of models for the estimation of mating systems using n independent loci. Heredity, 2002, 88: 221-228.
[17] Ritland K. Multilocus mating system program (MLTR). Version 3.0[CP/OL].[2009-10-15]. http://genetics.forestry.ubc.ca/ritland/programs.html.
[18] Karaswa M M G, Vencovsky R, Silva C M, et al. Mating system of Brazilian Oryza glumaepatula populations studied with microsatellite markers. Ann Bot, 2007, 99(2): 245-253.
[19] 董贞荣, 陈进明, 王青锋. 泽苔草交配系统的RAPD分析. 武汉植物学研究, 2006, 24(2): 167-170.
[20] 李斌, 顾万春. 白皮松交配系统及其种内遗传多样性分量比的研究. 林业科学研究, 2004, 17(1): 19-25.
[21] 李小湘, 詹庆才, 魏兴华, 等. 湖南江永普通野生稻原位和异位保存种质的SSR多样性差异. 中国水稻科学, 2006, 20(4): 361-366. 浏览
[22] 陈小勇, 林鹏. 厦门木麻黄种群交配系统及近交衰退. 应用生态学报, 2002, 13(11): 1377-1380.
[23] Chen L J, Lee D S, Song Z P, et al. Gene flow from cultivated rice (Oryza sativa) to its weedy and wild relatives. Ann Bot, 2004, 93(1): 67-73.
[24] Song Z P, Lu B R, Zhu Y G, et al. Pollen competition between cultivated rice to the wild species Oryza rufipogon under experimental field conditions. New Phytol, 2003, 157: 657-665.
[25] Wang F,Yuan H, Shi L, et al. A large-scale field study of transgene flow from cultivated rice (Oryza sativa) to common wild rice(O. rufipogon) and barnyard grass (Echinochloa crusgalli). Plant Biotechnol J, 2006, 4: 1-10.
[26] Ge S, Oliveira G C X, Schaal B A, et al. RAPD variation within and between natural populations of the wild rice Oryza rufipogon from China and Brazil. Heredity,1999, 82: 638-644.
[27] Song Z P, Zhu W Y, Rong J, et al. Evidences of introgression from cultivated rice to Oryza rufipogon (Poaceae) populations based on SSR fingerprinting: Implications for wild rice differentiation and conservation. Evol Ecol, 2006, 20: 501-522.
[28] Akimoto M, Shimamoto Y, Morishima H. The extinction of genetic resources of Asian wild rice,Oryza rufipogon Griff.: A case study in Thailand. Genet Res Crop Evol, 1999, 46: 419-425.
[29] 杨庆文, 戴陆园, 时津霞, 等. 云南元江普通野生稻(Oryza rufipogon Griff)遗传多样性分析及保护策略研究. 植物遗传资源学报, 2004, 5(1): 1-5.
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[J]. , 2011, 25(2): 175-181 .
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[4] JIANG Yun-zhu,TANG Shen-xiang,TANG Jian*. Genetic Structure and Geographical Distribution of Chinese Rice Cultivars Based on Allozymic Alleles [J]. , 2010, 24(5): 453-462 .
[5] HOU Li-yuan,YU Ping,XU Qun,YUAN Xiao-ping,YU Han-yong,WANG Yi-ping,WANG Cai-hong,WAN Guo,PENG Suo-tang,WEI Xing-hua . Genetic Analysis and Preliminary Mapping of Two Recessive Resistance Genes in Rice to Brown Planthopper, Nilaparvata lugens[J]. , 2010, 24(4): 367-371 .
[6] MA Lin,YU Xian-quan*,ZHAO Fu-sheng. SSR-Based Analysis on Genetic Diversity of Rice Landraces from Guizhou Province, China [J]. , 2010, 24(3): 237-243 .
[7] ZHAO Xingjuan,LIU Xiangdong,LI Jinquan*,LU Yonggen* . Construction of Single Segment Substitution Lines in Rice by Using Gaozhou Common Wild Rice(Oryza rufipogon) as Donor Parent[J]. , 2010, 24(2): 210-214 .
[8] XIA Zhi-hui,#,HAN Fei,#,GAO Li-fen,YUAN Qian-hua,ZHAI Wen-xue, LIU Di,LUO Yue-hua. Application of Functional Marker to Identify Genes for Bacterial Blight Resistance in Oryza rufipogon [J]. , 2009, 23(6): 653-656 .
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[10] CHENG Ben-yi,WU Wei,XIA Jun-hui,LIU Xin,YANG Shi-hua. Microsatellite Marker Analysis on Genetic Diversity of Main Rice Varieties in Zhejiang Province, China[J]. , 2009, 23(5): 551-554 .
[11] LI Hongyu,HOU Yuming,CHEN Yinghua,XU Zhengjin*,CHEN Wenfu,ZHAO Minghui,MA Dianrong,XU Hai,WANG Jiayu. Evaluation on Genetic Diversity of the Commercial Rice Varieties in Northeast China by Microsatellite Markers
[J]. , 2009, 23(4): 383-390 .
[12] WEI Yanping,HUANG Dahui,CHEN Yingzhi,LIU Chi,YANG Lang,LUO Xuemei,MA Zengfeng,ZHANG Yuexiong,LIU Yali,YANG Xinqing,LI Rongbai,. Identification and Evaluation of Resistance to Rice Blast in Guangxi Wild Rice Resources[J]. , 2009, 23(4): 433-436 .
[13] YU Ping,LI Li,LU Jianzhen,YUAN Xiaoping,XU Qun,WANG Yiping,YU Hanyong,WEI Xinghua. SSR Analysis on japonica Rice Landraces from the Taihu Lake Region, China[J]. , 2009, 23(2): 148-148~152 .
[14] PENG Suo-tang,WANG Hai-gang,WEI Xing-hua*,LU Jian-zhen,ZHANG Xiao-li,YUAN Xiao-ping,YANG Wu-de. SSR Diversity and Genetic Structure of Main Male Sterile Lines of Three-Line Hybrid Rice in China[J]. , 2008, 22(4): 365-369 .
[15] LIAN Zi-xian,WEI Chang-min,LU Yong-gen,ZHAO Xing-juan,FU Xue-lin,LI Jin-quan,YANG Bing-yao,LIU Xiang-dong*. Fertility and Development Charateristics of Embryo Sac in F-1 Hybrids between Oryza sativa Subsp. japonica and Oryza rufipogon Indigenous to Gaozhou, Guangdong Province, China[J]. , 2008, 22(3): 266-272 .
[16] LU Jian-zhen,ZHANG Xiao-li,WANG Hai-gang,YUAN Xiao-ping,XU Qun,WANG Yi-ping,YU Han-yong,WEI Xing-hua*. SSR Analysis on Diversity of AA Genome Oryza Species in the Southeast and the South Asia[J]. , 2008, 22(3): 249-254 .
[17] GONG Ji-ping ,WU Fang-xi ,WU Yue-jin ,ZHENG Jia-tuan ,HUANG Ting-xu ,WANG Wu-qi ,ZHANG Jian-fu ,XIE Hua-an . Mapping and Genetic Analysis of a Lipase Gene for indica Rice [J]. , 2008, 22(2): 125-130 .
[18] WANG Yi-ping ,WEI Xing-hua ,YUAN Xiao-ping ,YU Han-yong ,XU Qun ,TANG Sheng-xiang. Analysis on Genetic Diversity of Natural Populations of Oryza rufipogon Distributed in Hainan Province by SSR Markers[J]. , 2007, 21(6): 573-578 .
[19] DENG Hua-bing ,DENG Qi-yun ,CHEN Li-yun ,YANG Yi-shan ,XIONG Yue-dong ,KONG Fan-na ,WANG Bin ,YUAN Long-ping. Marker Assisted Selection for Yield Enhancing QTLs in the Progeny of 9311× Oryza rufipogon and Its Effects in Rice Breeding[J]. , 2007, 21(6): 605-611 .
[20] LI Ya ,LIU Er-ming ,DAI Liang-ying ,LI Cheng-yun ,LIU Lin. Genetic Diversity among Populations as Related to Pathotypes for Magnaporthe grisea in Hunan Province[J]. , 2007, 21(3): 304-308 .
[21] HUA Lei ,YUAN Xiao-ping ,YU Han-yong ,WANG Yi-ping ,XU Qun ,TANG Sheng-xiang ,WEI Xing-hua. A Comparative Study on SSR Diversity in Chinese Major Rice Varieties Planted in 1950s and During the Most Recent Ten Years[J]. , 2007, 21(2): 150-154 .
[22] YANG Zhi-rong ,LI Run-zhi ,WEI Xing-hua. SSR Analysis on Diversity and Genetic Relationship Among Eight AA-genome Oryza Species [J]. , 2006, 20(6): 589-595 .
[23] WAN Ya-tao ,A Xin-xiang ,FAN Chuan-zhang ,XU Fu-rong ,TAN Xue-lin ,YU Teng-qiong ,TANG Cui-feng ,DAI Lu-yuan. ISSR Analysis on Genetic Diversity of the 34 Populations of Oryza meyeriana Distributed in Yunnan Province [J]. , 2006, 20(6): 596-602 .
[24] WANG Sheng-jun ,LU Zuo-mei ,WAN Jian-min. Genetic Diversity of Parental Lines in indica Hybrid Rice Based on Phenotypic Characters and SSR Cluster Analysis[J]. , 2006, 20(5): 475-480 .
[25] CHENG Gui-ping ,FENG Jiu-huan ,LIANG Guo-hua ,LIU Xiang-dong ,LI Jin-quan. Identification of QTLs for Agronomic Traits Associated with Yield in a BC2F2 Population Between Oryza sativa and Oryza rufipogon[J]. , 2006, 20(5): 553-556 .
[26] LI Xiao-xiang ,ZHAN Qing-cai ,WEI Xing-hua ,DUAN Yong-hong ,CHEN Zu-wu ,LIU Yong. SSR Diversity of in-situ and ex-situ Conserved Oryza rufipogon Populations in Jiangyong County of Hunan Province[J]. , 2006, 20(4): 361-366 .
[27] LI Ya-li ,YANG Xiao-xi ,ZHAO Feng-ping ,XU Ming-hui. SSR Markers on indica-japonica Differentiation of Natural Population of Oryza rufipogon in Yuanjiang, Yunnan Province [J]. , 2006, 20(2): 137-140 .
[28] WANG Lin ,DAI Lu-yuan ,WU Li-hua ,YANG Qing-wen ,XU Fu-rong ,YU Teng-qiong ,TANG Cui-feng. Comparative Analysis and Investigation on Plants Growing in in situ Environments of Three Wild Rice Species in Yunnan Province[J]. , 2006, 20(1): 47-52 .
[29] XIAO Xiao-yu ,WANG Yu-ping ,ZHANG Jian-yong ,LI Shi-gui ,RONG Ting-zhao. SSR Marker-based Genetic Diversity Fingerprinting of Hybrid Rice in Sichuan,China[J]. , 2006, 20(1): 1-7 .
[30] YANG Qing-wen ,CHEN Cheng-bin ,ZHANG Wan-xia ,SHI Jin-xia ,REN Jun-fang . Minimum Number of SSR Alleles Needed for Genetic Structure Analysis of Oryza rufipogon Populations [J]. , 2005, 19(4): 297-302 .
[31] CHEN Zhong-zhong ,WANG Xu-sheng ,ZHU Jun . Analysis of SSR Polymorphism by Genome-Scale Comparing Between Varieties in Rice [J]. , 2005, 19(4): 303-307 .
[32] WANG Yan-li ,Claudia KAYE ,Amandine BORDAT ,Henri ADREIT ,Jo(e)lle MILLAZZO ,ZHENG Xiao-bo ,SHEN Ying ,Didier THARREAU . Construction of Genetic Linkage Map and Location of Avirulence Genes from Cross CH63 and TH16 of Magnaporthe grisea [J]. , 2005, 19(2): 160-166 .
[33] SHEN Ying ,Julien FROUIN ,HE Yue-qiu ,Claudia KAYE ,XIAO Fang-hua ,Jean Loup NOTTEGHEM ,LIU Er-ming ,Didier THARREAU . The Perfect Stage and SSR Analysis of Magnaporthe grisea in the Yanxi Blast Nursery, Hunan Province [J]. , 2004, 18(3): 262-268 .
[34] JI Guang-hai,QIAN Jun,ZHANG Shi-guang,WEI Lan-fang,CHEN Xing-quan. Genetic Diversity of Rice Cultivars Resistant to Bacterial Blight in Yunnan[J]. , 2003, 17(2): 118-122 .
[35] WEI Xing-hua ,TANG Sheng-xiang ,JIANG Yun-zhu ,YU Han-yong ,QIU Zong-en ,YAN Qi-chuan . Genetic Diversity of Allozyme Associated with Morphological Traits in Chinese Improved Rice Varieties[J]. , 2003, 17(2): 123-128 .
[36] LI Rong-bai ,QIN Xue-yi ,WEI Su-mei ,HUANG Feng-kuan ,LI Qing ,LUO Shan-yu . The High Resistance and Its Inheritance of 94-42-5-1, a Source Derived from Oryza rufipogon to Brown Planthopper, Nilaparvata lugens[J]. , 2002, 16(2): 115-118 .
[37] WANG Chun-lian,ZHANG Qi,ZHOU Yong-li,ZHAO Bing-yu. Genetic Diversity of Pathogen Xanthomonas oryzae pv. oryzae from Southern Regions of Yangtze River in China[J]. , 2001, 15(2): 131-136 .
[38] Liu Guodong,Liu Gengling. Genotypic Differences in Potassium Contents of Rice Seeds [J]. , 1997, 11(3): 179-182 .
[39] Zhuang Jieyun,Qian Huirong,Lin Hongxuan,Lu Jun,Cheng Shihua,Ying Cunshan,Luo Lijun,Zhu Xudong,Dong Fenggao,Min Shaokai,Sun Zongxiu,Zheng Kangle. RFLP-based Analysis of the Origin and differentiation of Oryza sativa L.[J]. , 1995, 9(3): 135-140 .
[40] Pang Hanhua,Tang Shengxiang. Study on Anther Culture Ability of different Ecotypes of O. rufipogon Griff[J]. , 1995, 9(3): 167-171 .
[41] Zhang Qi,Wang Chunlian,Jan E. Leach,R J Nelson,T W Mew . Preliminary Analysis of the Population Structure of Xanthomonas oryzae pv. oryzae in China [J]. , 1995, 9(1): 7-14 .
[42] Tan Yujuan,Zhang Yang,Pan Ying,Huang Bingchao. Study on the Resistance to Yellow Stem Borer and Brown Planthopper in a Common Wild Rice SB1[J]. , 1993, 7(4): 246-246 .
[43] Tan Yujuan,Zhang Yang,Pan Ying,Liu Xuezhen,Chen Jinde. Evaluation of Guangdong Wild Rice Germplasm for Resistance to Whitebacked Planthopper [J]. , 1993, 7(1): 42-42 .
[44] Lin Shicheng,Zhang Qi,Que Gengsheng,Xing Zuyi,Wang Chunlian,Li Daoyuan,Chen Chengbin. Evaluation and Genetic Analysis for Resistance to Bacterial Blight in Wild Rice [J]. , 1992, 6(4): 155-158 .
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