Comparative Analysis of Genomes of Oryza alta  and Oryza latifolia by Using C0t-1  DNA

doi:10.3969/j.issn.1001-7216.2010.02.05

Abstract

Abstract: In order to reveal the origin and evolutionary relationship between two CCDD genome species of rice, Oryza alta and Oryza latifolia, fluorescence in situ hybridization (FISH) procedure was adopted to analyse the genomes of O. alta and O. latifolia using C0t1 DNA from O. alta as a probe. Karyotype analysis between O. alta and O. latifolia was also made comparatively based on their similar band patterns of the hybridization signals. There was a high homology and close relationship between O. alta and O. latifolia, but at the same time, the distinction between the hybridization signals was also clear. This indicates that C0t1 DNA was species and genome typespecific, and C0t1 DNAFISH could be more efficient in analysis of the genome relationship between different species. According to the comparative analysis of highly and moderately repetitive DNA sequences between the two allotetraploidy species, O. alta and O.latifolia, the possible origin and evolutionary mechanism of allotetraploidy of Oryza were discussed.

Key words: Oryza alta, Oryza latifolia, chromosomal repeat sequence, fluorescence in situ hybridization, karyotype, genome, phylogenetic relation

摘要： 为了揭示同为CCDD基因组类型的高秆野生稻和宽叶野生稻的起源和进化关系，用高秆野生稻C0t1 DNA作为探针，对其自身体细胞染色体与宽叶野生稻体细胞染色体进行荧光原位杂交并对其核型进行同源性聚类和比较分析。杂交结果显示宽叶野生稻和高秆野生稻同源性非常高，说明它们的亲缘关系十分接近，但区别也非常明显，两者的杂交信号存在显著不同。C0t1 DNA具有很强的种特异性和依赖基因组型的特异性，利用它进行荧光原位杂交能够更有效地研究物种基因组间的关系，为物种的划分和鉴定提供依据。另外，通过对高秆野生稻和宽叶野生稻两个异源四倍体基因组的中高度重复序列的比较分析，探讨了稻属异源四倍体可能的起源与进化机制。

关键词: 高秆野生稻, 宽叶野生稻, 染色体重复序列, 荧光原位杂交, 核型, 基因组, 亲缘关系

WANG Debin, WANG Yang,ZHAO Houming, LI Gang, QIN Rui,LIU Hong*. Comparative Analysis of Genomes of Oryza alta and Oryza latifolia by Using C0t-1 DNA [J]. Chinese Journal of Rice Science, DOI: 10.3969/j.issn.1001-7216.2010.02.05 .

王德彬,王阳,赵侯明,李刚,覃瑞,刘虹. 利用C0t-1 DNA对高秆野生稻和宽叶野生稻基因组的比较分析[J]. 中国水稻科学, DOI: 10.3969/j.issn.1001-7216.2010.02.05 .

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Comparative Analysis of Genomes of Oryza alta and Oryza latifolia by Using C0t-1 DNA

利用C0t-1 DNA对高秆野生稻和宽叶野生稻基因组的比较分析

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