Breeding of Cytoplasmic Male Sterile Line Derived from Oryza sativa subsp. javanica cv.  Wanilava and Its AFLP Fingerprints

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Breeding of Cytoplasmic Male Sterile Line Derived from Oryza sativa subsp. javanica cv.
Wanilava and Its AFLP Fingerprints

CHEN Yong-jun , XIE Chong-hua , ZHANG Jing-rong , ZHENG Wen-jing , HU Shang-lian , WEI Xin , WANG Qi-lin , QI Cong-ting , LIU Yong-sheng

Received:1900-01-01 Revised:1900-01-01 Online:2008-01-10 Published:2008-01-10
Contact: LIU Yong-sheng

爪哇稻Wanilava细胞质雄性不育系的创建及AFLP指纹图谱分析

陈永军1;谢崇华1;张景荣2;郑文静2;胡尚连1;魏炘2;王琦琳2;杞从婷2;刘永胜1,2,*

1西南科技大学生命科学与工程学院，四川绵阳621002； 2四川大学生命科学院生物资源与生态环境教育部重点实验室，四川成都610064

Abstract

Abstract: The characteristics of a novel cytoplasmic male sterile (CMS) line derived from Oryza sativa subsp. javanica cv. Wanilava (denominated as JWtype) were analyzed. Two sets of isonucleusallocytoplasmic lines (273D, 273JW and 273B； 803WA, 803JW and 803B, respectively) were bred by advanced backcrosses using the maintainer lines (273B and 803B, respectively) as recurrent parents, each containing individual cognate nucleus with different cytoplasms. Considerable genetic diversities between CMSJW (CMS from javanica cv. Wanilava) and CMSWA (CMS of wild abortive from O. rufipogon) or CMSD (CMS from African indica cv. Dissi) were revealed by AFLP. The differential fragments from the cytoplasms were isolated.

Key words: cytoplasmic male sterility, isonucleusallocytoplasmic lines, amplified fragment length polymorphism, fingerprint, javanica rice

摘要： 初步鉴定了爪哇稻Wanilava细胞质雄性不育系的特征特性，并以2套水稻（Oryza sativa）同核异质材料273JW（爪哇型胞质不育系）、273D（D型胞质不育系）及其对应的保持系273B， 803JW（爪哇型胞质不育系）、803WA（野败型胞质不育系）及其对应的保持系803B为研究对象，利用AFLP 分子标记技术构建了JW型不育胞质的指纹图谱，分离了JW型胞质与野败型胞质及保持系的差异片段。

关键词: 细胞质雄性不育, 同核异质材料, 扩增片段长度多态性

CHEN Yong-jun ,XIE Chong-hua ,ZHANG Jing-rong ,ZHENG Wen-jing ,HU Shang-lian ,WEI Xin ,WANG Qi-lin ,QI Cong-ting ,LIU Yong-sheng. Breeding of Cytoplasmic Male Sterile Line Derived from Oryza sativa subsp. javanica cv.
Wanilava and Its AFLP Fingerprints[J]. Chinese Journal of Rice Science.

陈永军,谢崇华,张景荣,郑文静,胡尚连,魏炘,王琦琳,杞从婷,刘永胜. 爪哇稻Wanilava细胞质雄性不育系的创建及AFLP指纹图谱分析[J]. 中国水稻科学.

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