Abstract:

The pot and field experiments for studying the multiplication techniques of rice TGMS lines with cold water irrigation were conducted, in which an indica rice TGMS line GD-2S with low critical temperature of fertility transition was used as experimental materials. The results suggested that: 1) It was the best to start the cold water irrigation treatment at the phase of differentiation of secondary rachis branch and spikelet primordia. But the sensitive stage of TGMS line GD-2S was during 11th day to 19th day before its heading, namely, from the differentiation of stamen and pistil primordia (phase Ⅳ ) to meiotic division of the pollen mother cell (phase Ⅵ). There seemed to exist a starting process for the rice plants to respond to cold water treatment. 2) It was suitable to be treated with cold water irrigation for about 13 d. Cold water treatment would cause the spikelets degeneration, prolong the developing process of the plants and delay the heading time. The longer the cold water irrigation or the earlier the treatment start, the more the degenerative spikelets. 3) The suitable irrigation depth was about 12cm, Just up to the growth point of the plant.

Key words: cold water irrigation, multiplication techniques, TGMS line, two-line hybrid rice

摘要： 通过盆栽和大田试验，研究了籼型低温敏核不育系GD-2S的冷灌繁种技术。结果表明，1)冷灌开始时期以幼穗分化Ⅲ期(二次枝梗和颖花原基分化)为好。但是，GD-2S的温度诱导育性转换的敏感期在抽穗前的第11天至19天，即雌雄蕊形成期(Ⅳ期)至花粉母细胞减数分裂期(Ⅵ期)。冷灌处理开始至植株产生反应之间存在一个启动过程。2)冷灌天数以13 d左右为宜。冷灌处理会导致颖花退化，延缓生育进程，推迟抽穗时间。冷灌开始时期越早或冷灌时间越长，颖花退化越严重(如冷灌20 d的达25.5%)，结实率也降低。3)冷灌深度以12 cm左右(刚浸至生长点)深的结实率最高。

关键词: 两系杂交稻, 低温敏核不育系, 冷水灌溉, 繁种技术