Abstract: In the first 20 days after subculture, the growth rate of rice calli was relative high; the contents of their nucleic acids and proteins were increasing and maintaining at relative high level;specific activities of their ribonuclease and hemoglobin-digesting proteinase were decreasing and keeping at relative low level. So, it was suggested to subculture rice calli within 20 days. According to morphologic and metabolic characteristics, it was proposed that the growth process of calli after subculture may be divided into vigorous growth, stable growth and senescing stages. There was a close relationship between content of nucleic acids and specific activity of ribonuclease or content of proteins and specific activity of hemoglobin-digesting proteinase. So it was suggested that ribonuclease and hemoglobin-digesting proteinase might play an active role in lowering the contents of nucleic acids and proteins of rice calli respectively after vigorous growth stage.

Key words: Informational macromolecules: Growth and senescence, Rice calli

摘要： 水稻愈伤组织转移继代培养后的0—20天,生长速率较快,核酸和蛋白质含量急剧增加,且维持较高水平,核糖核酸酶和血红蛋白水解酶活性急剧下降,且维持较低活性。因此,愈伤组织的继代培养时间最好掌握在生长速率较快,核酸蛋白质含量较高、水解酶类活性较低的阶段,即在20天以内。核酸和蛋白质含量与其水解酶类(核糖核酸酶和血红蛋白水解酶)的比活之间存在着密切的关系,从而认为核糖核酸酶和血红蛋白水解酶分别在核酸和蛋白质含量下降中起着积极作用。根据信息大分子化合物的变化动态和愈伤组织外部形态变化提出愈伤组织转移继代培养过程可以分为三个阶段,即旺盛生长、稳定生长和衰老阶段。

关键词: 信息大分子化合物, 生长和衰老, 水稻愈伤组织