Chinese Journal OF Rice Science ›› 2017, Vol. 31 ›› Issue (4): 441-446.DOI: 10.16819/j.1001-7216.2017.6165 441
• Orginal Article • Previous Articles
Yan LIANG1, Zhijuan JI1, Yuxiang ZENG1, Cuizhen ZHANG2, Hanlin WU1, Changdeng YANG1,*()
Received:
2016-09-21
Revised:
2017-03-23
Online:
2017-07-25
Published:
2017-07-10
Contact:
Changdeng YANG
梁燕1, 季芝娟1, 曾宇翔1, 张翠真2, 吴晗霖1, 杨长登1,*()
通讯作者:
杨长登
基金资助:
Yan LIANG, Zhijuan JI, Yuxiang ZENG, Cuizhen ZHANG, Hanlin WU, Changdeng YANG. Genealogical Analysis on Resistance to Magnaporthe oryzae of Super Early Rice Zhongzao 39[J]. Chinese Journal OF Rice Science, 2017, 31(4): 441-446.
梁燕, 季芝娟, 曾宇翔, 张翠真, 吴晗霖, 杨长登. 超级早稻中早39稻瘟病抗性的系谱分析[J]. 中国水稻科学, 2017, 31(4): 441-446.
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URL: http://www.ricesci.cn/EN/10.16819/j.1001-7216.2017.6165 441
名称Name | 引物序列 Primer sequence (5′-3′) | 目的片段大小Product/bp | 目的基因 Target gene | 退火温度 Annealing temperature /℃ | 参考文献 Reference | |
---|---|---|---|---|---|---|
pid2-F | TTGGCTATCATAGGCGTCC | 1057 | Pi-d2 | 55 | [13] | |
pid2-R | ATTTGAAGGCGTTTGCGTAGA | |||||
pid3-F | GAATGAGTGGAATCGGCAAG | 775 | Pi-d3 | 55 | [13] | |
pid3-R | AATCCTTCAGCAACCCAGAG | |||||
pi36-F | CAATGTGTGACTTGTGCGGACT | 1036 | Pi36 | 55 | [13] | |
pi36-R | TCTTCCATCTCGGATTTCGTGT | |||||
Piz-F | GGACCCGCGTTTTCCACGTGTAA | 292 | Piz | 60 | [14] | |
Piz-R | AGGAATCTATTGCTAAGCATGAC | |||||
Pib-F | ATGGAGGATACCGAGTTGACTG | 1089 | Pib | 55 | [13] | |
Pib-R | CCGTTGACTTTGAGATGGCGAT | |||||
YRT6-F | TCCTGTGTTTCCTACCGAGTCCAGC | 1016 | Pi64 | 55 | [15] | |
YRT6-R | AGAGGAGTGCAAGGTTACCAGAGCC | |||||
P9-F | CGGTTATAGATGAATATAGTTC | 803 | Pi9 | 50 | [16] | |
P9-R | GTTCGACTCTCCCTTCAAGC | |||||
Pid2-2F* | TTGGGTCTAGACCAAACAGAGCTTCTAACAATC | 2720 | Pi-d2 | 55 | [8] | |
Pid2-2R* | TTTGCGTCGACCTAGTTACAGATCACTGTGCCAT |
Table 1 Primer used for molecular marker screening and gene sequencing analysis.
名称Name | 引物序列 Primer sequence (5′-3′) | 目的片段大小Product/bp | 目的基因 Target gene | 退火温度 Annealing temperature /℃ | 参考文献 Reference | |
---|---|---|---|---|---|---|
pid2-F | TTGGCTATCATAGGCGTCC | 1057 | Pi-d2 | 55 | [13] | |
pid2-R | ATTTGAAGGCGTTTGCGTAGA | |||||
pid3-F | GAATGAGTGGAATCGGCAAG | 775 | Pi-d3 | 55 | [13] | |
pid3-R | AATCCTTCAGCAACCCAGAG | |||||
pi36-F | CAATGTGTGACTTGTGCGGACT | 1036 | Pi36 | 55 | [13] | |
pi36-R | TCTTCCATCTCGGATTTCGTGT | |||||
Piz-F | GGACCCGCGTTTTCCACGTGTAA | 292 | Piz | 60 | [14] | |
Piz-R | AGGAATCTATTGCTAAGCATGAC | |||||
Pib-F | ATGGAGGATACCGAGTTGACTG | 1089 | Pib | 55 | [13] | |
Pib-R | CCGTTGACTTTGAGATGGCGAT | |||||
YRT6-F | TCCTGTGTTTCCTACCGAGTCCAGC | 1016 | Pi64 | 55 | [15] | |
YRT6-R | AGAGGAGTGCAAGGTTACCAGAGCC | |||||
P9-F | CGGTTATAGATGAATATAGTTC | 803 | Pi9 | 50 | [16] | |
P9-R | GTTCGACTCTCCCTTCAAGC | |||||
Pid2-2F* | TTGGGTCTAGACCAAACAGAGCTTCTAACAATC | 2720 | Pi-d2 | 55 | [8] | |
Pid2-2R* | TTTGCGTCGACCTAGTTACAGATCACTGTGCCAT |
菌株名称 Isolate name | 抗性 Resistance | 种群 Group | ||||||
---|---|---|---|---|---|---|---|---|
特特普 Tetep | 珍龙13 Zhenlong 13 | 四丰43 Sifeng 43 | 东农363 Dongnong 363 | 关东51 Guandong 51 | 合江18 Hejiang 18 | 丽江新团黑谷 Lijiangxintuanheigu | ||
14-754 | 抗R | 感S | 抗R | 抗R | 抗R | 抗R | 感S | ZB31 |
10-105 | 感S | 抗R | 抗R | 感S | 感S | 感S | 感S | ZA49 |
12-157 | 抗R | 感S | 感S | 抗R | 抗R | 感S | 感S | ZB13 |
16-754 | 抗R | 感S | 抗R | 抗R | 抗R | 感S | 感S | ZB29 |
16-161 | 抗R | 抗R | 抗R | 抗R | 抗R | 抗R | 感S | ZG1 |
Table 2 The resistance of the seven varieties to the tested Magnaporthe oryzae isolates.
菌株名称 Isolate name | 抗性 Resistance | 种群 Group | ||||||
---|---|---|---|---|---|---|---|---|
特特普 Tetep | 珍龙13 Zhenlong 13 | 四丰43 Sifeng 43 | 东农363 Dongnong 363 | 关东51 Guandong 51 | 合江18 Hejiang 18 | 丽江新团黑谷 Lijiangxintuanheigu | ||
14-754 | 抗R | 感S | 抗R | 抗R | 抗R | 抗R | 感S | ZB31 |
10-105 | 感S | 抗R | 抗R | 感S | 感S | 感S | 感S | ZA49 |
12-157 | 抗R | 感S | 感S | 抗R | 抗R | 感S | 感S | ZB13 |
16-754 | 抗R | 感S | 抗R | 抗R | 抗R | 感S | 感S | ZB29 |
16-161 | 抗R | 抗R | 抗R | 抗R | 抗R | 抗R | 感S | ZG1 |
材料 Material | 14-754 | 10-105 | 12-157 | 16-754 | 16-161 |
---|---|---|---|---|---|
浙辐802 Zhefu 802 | 抗R | 抗R | 感S | 感S | 抗R |
嘉育293 Jiayu 293 | 抗R | 抗R | 感S | 感S | 抗R |
湘早籼1号 Xiangzaoxian 1 | 抗R | 抗R | 抗R | 抗R | 抗R |
青谷矮3号 Qinggu'ai 3 | 抗R | 抗R | 抗R | 抗R | 抗R |
陆青早1号 Luqingzao 1 | 抗R | 抗R | 抗R | 抗R | 抗R |
金早47 Jinzao 47 | 感S | 抗R | 抗R | 抗R | 抗R |
中组3号 Zhongzu 3 | 感S | 抗R | 抗R | 抗R | 抗R |
嘉育253 Jiayu 253 | 感S | 抗R | 抗R | 感S | 抗R |
中早39 Zhongzao 39 | 感S | 抗R | 抗R | 抗R | 抗R |
地谷Digu | 感S | 抗R | 抗R | 抗R | 抗R |
Table 3 Blast resistance of the materials tested.
材料 Material | 14-754 | 10-105 | 12-157 | 16-754 | 16-161 |
---|---|---|---|---|---|
浙辐802 Zhefu 802 | 抗R | 抗R | 感S | 感S | 抗R |
嘉育293 Jiayu 293 | 抗R | 抗R | 感S | 感S | 抗R |
湘早籼1号 Xiangzaoxian 1 | 抗R | 抗R | 抗R | 抗R | 抗R |
青谷矮3号 Qinggu'ai 3 | 抗R | 抗R | 抗R | 抗R | 抗R |
陆青早1号 Luqingzao 1 | 抗R | 抗R | 抗R | 抗R | 抗R |
金早47 Jinzao 47 | 感S | 抗R | 抗R | 抗R | 抗R |
中组3号 Zhongzu 3 | 感S | 抗R | 抗R | 抗R | 抗R |
嘉育253 Jiayu 253 | 感S | 抗R | 抗R | 感S | 抗R |
中早39 Zhongzao 39 | 感S | 抗R | 抗R | 抗R | 抗R |
地谷Digu | 感S | 抗R | 抗R | 抗R | 抗R |
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