Clustering of Major Genes Conferring Blast Resistance in Rice Cultivar Gumei 2
WU Jian-li1; 3; 4; CHAI R ong-yao2; FAN Ye-yang1; LI De-bao3; ZHENG Kang-le1; Hei LEUNG4; ZHUANG Jie-yun1; *
1State Key Laboratory of Rice Biology; Chinese National Center for Rice Improvement; China National Rice Research Institute; Hangzhou 310006; China; 2Plant Protection Institute; Zhejiang Academy of Agricultural Sciences; Ha ngzhou 310021; 3Institute of Biotechnology; Zhejiang University; Hangz hou 310029; 4 Entomology and Plant Pathology Division; International Rice Research Institute, Manila, Philippines.
By using 304 recombinant inbred lines derived fro m indica rice cross Zhong 156/Gumei 2, a linkage map consisting of 177 marker loci and covering 12 rice chromosomes was constructed and employed for mapping genes conferring blast resistance in rice. Genomic location of gene Pi25(t) conferring neck blast resistance to Chinese isolate 92-183 (race ZC15) was verified to be located between markers A7 and RG456 on chromosome 6, with genetic distances of 1.7 cM and 1.5 cM to A7 and RG456, respe ctively. Leaf blast resistance of Gumei 2 to Philippine isolate Ca89 (lineage 4 ) was found to be controlled by a single gene. The gene tentatively designated as Pi26(t) was located between markers B10 and R674 on chromosome 6, with genetic distances of 5.7 cM and 25.8 cM to B10 and R674 , respectively. Resistant alleles at both gene loci were derived from Gumei 2, indicating an existence of resistance gene clusters in Gumei 2.