中国水稻科学

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云南元江普通野生稻(Oryza rufipogon)群体籼粳分化的SSR分析

李亚莉1;杨晓曦2;赵丰萍3; 许明辉3,*   

  1. 1中国科学院 昆明植物研究所, 云南 昆明 650204; 2云南省环境保护局 珍稀植物引种繁育中心, 云南 昆明 650032; 3云南省农业科学院 生物技术与种质资源研究所, 云南 昆明 650223
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-03-10 发布日期:2006-03-10

SSR Markers on indica-japonica Differentiation of Natural Population of Oryza rufipogon in Yuanjiang, Yunnan Province

LI Ya-li , YANG Xiao-xi , ZHAO Feng-ping , XU Ming-hui   

  • Received:1900-01-01 Revised:1900-01-01 Online:2006-03-10 Published:2006-03-10

摘要: 利用经测验可区分籼粳品种的19对SSR特异引物对来自云南元江普通野生稻自然居群的56个个体进行了SSR分析。19对引物中,有17对(占89.47%)在所有参试个体中仅能扩增出一种带型,而RM18和RM251能扩增出多态带型。RM4等16对(84.21%)引物扩增出的带与籼稻或粳稻特征带相同,其中11个位点偏粳而4个位点偏籼;RM18、RM202、RM205等3对引物扩增出的带型不同于籼稻或粳稻带型。结果表明,云南元江普通野生稻基因组DNA在所检测的19个位点上,多数位点(89.47%)上个体间无差异,84.21%的位点已有籼粳分化,13.79%的位点仍具有原始性。说明云南元江普通野生稻主体比较纯而原始,但已开始了籼粳的分化。

关键词: 普通野生稻, 籼粳分化, 微卫星标记

Abstract: By using 19 pairs of SSR primers which could identify indica and japonica types of cultivated rice (Oryza sativa), the indica-japonica differentiation of 56 individuals from the natural population of common wild rice (Oryza rufipogon) in Yuanjiang was analyzed. Of the 19 pairs of primers, 17 pairs (89.47%) could amplify only one kind of band type among all the individuals, but primers RM251 and RM18 could amplify polymorphic band types. The bands amplified by 16 pairs of primers (84.21 %) were similar with the indica-japonica identified bands of relevant locus in cultivated rice including 11 japonica-like loci and 4 indica-like loci, while the bands amplified by other 3 pairs of primers (RM18, RM202, RM205) were different from indica or japonica identified bands of cultivated rice. Among the 19 loci analyzed, 84.21% of them in genomic DNA of common wild rice in Yuanjiang showed indica-japonica differentiation and 13.79% of them still kept primitive, and the natural population was homogenetic in most of the detected loci.

Key words: Oryza rufipogon, indica-japonica differentiation, simple sequence repeat, population