水稻千粒重QTL qTGW1.1的验证

doi:10.3969/j.issn.1001-7216.2014.02.003

中国水稻科学 ›› 2014, Vol. 28 ›› Issue (2): 125-131.DOI: 10.3969/j.issn.1001-7216.2014.02.003

水稻千粒重QTL qTGW1.1的验证

张宏伟,朱玉君,陈玉宇,陈俊宇,黄得润,庄杰云^*

中国水稻研究所国家水稻改良中心/水稻生物学国家重点实验室, 杭州 310006；

收稿日期:2013-11-18 修回日期:2013-12-21 出版日期:2014-03-10 发布日期:2014-03-10
通讯作者: 庄杰云*
基金资助:
国家863计划资助项目（2012AA101102）；国家超级稻育种专项（201301）；中央级公益性科研院所基本业务费专项（2012RG0023)

Validation of qTGW1.1, a Quantitative Trait Locus for 1000grain Weight in Rice (Oryza sativa L.)

ZHANG Hongwei, ZHU Yujun, CHEN Yuyu, CHEN Junyu, HUANG Derun, ZHUANG Jieyun^*

Chinese National Center for Rice Improvement/State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China;

Received:2013-11-18 Revised:2013-12-21 Online:2014-03-10 Published:2014-03-10
Contact: ZHUANG Jieyun*

摘要/Abstract

摘要： 对前期定位在水稻第1染色体长臂上3.7 Mb区间内的千粒重QTL qTGW1.1进行验证，并提高其定位精度。从珍汕973/密阳46 BC2F5群体中筛选到1个在RM11448-RM11615区间杂合的单株，应用SSR标记检测其衍生的BC2F6和BC2F7群体，筛选得到杂合区间分别为RM11448-RM11522、RM11448-RM11549和RM1232-RM11615的BC2F7单株各1个。种植3个BC2F8群体，筛选在整个分离区间呈单一亲本型的单株，自交后获得在对应区间呈两种基因型的近等基因系各1套，大田种植，考查抽穗期、千粒重、粒长和粒宽，应用SAS软件对同一群体中不同基因型间的表型差异进行方差分析。在千粒重和粒形性状上，异质区间为RM11448-RM11522的近等基因系未呈显著变异，而另2套材料检测到显著的基因型作用，且其增效等位基因均来自珍汕97；对抽穗期，3套材料均未检测到显著作用。通过比较3个异质区间的基因组位置，将qTGW1.1界定于RM11522和RM11554之间688.8 kb的区间内。

关键词: 水稻（Oryza sativa L.）, 数量性状座位, 微效作用, 千粒重, 验证

Abstract: This study was conducted to validate a quantitative trait locus (QTL) for 1000grain weight (TGW) which was previously mapped in a 3.7 Mb region on the long arm of rice chromosome 1, and to locate this QTL more precisely. A plant carrying a heterozygous segment extending from RM11448 to RM11615 was selected from the Zhenshan 973/Milyang 46 BC2F5 population. The resulting BC2F6 and BC2F7 populations were assayed with SSR markers. Three BC2F7 plants were identified, carrying heterozygous segments covering the intervals RM11448-RM11522, RM11448-RM11549 and RM1232-RM11615, respectively. Nonrecombinant homozygotes were identified from the BC2F8 populations, from which three sets of near isogenic lines (NILs) in the BC2F8∶9 generation were produced, consisting of two parental genotypes in the intervals RM11448-RM11522, RM11448-RM11549 and RM1232-RM11615, respectively. Field trials were conducted to measure heading date (HD), TGW, and grain shape traits including grain length and width. Twoway analysis of variance was performed to test the phenotypic differences between two genotypic groups in each NIL set using SAS program. No significant variations were observed on TGW and grain shape in the NIL which was heterogeneous in the interval RM11448-RM11522, whereas significant genotypic effects were detected in the remaining two NILs in which the enhancing alleles were all derived from Zhenshan 97. For HD, no significant effects were detected in the three NILs. Based on comparison among genomic locations of the three heterogeneous regions, qTGW1.1 was delimited to a 688.8 kb region flanked by RM11522 and RM11554.

Key words: rice (Oryza sativa L.), quantitative trait locus, minor effect, 1000grain weight, validation

中图分类号:

张宏伟,朱玉君,陈玉宇,陈俊宇,黄得润,庄杰云*. 水稻千粒重QTL qTGW1.1的验证[J]. 中国水稻科学, 2014, 28(2): 125-131.

ZHANG Hongwei, ZHU Yujun, CHEN Yuyu, CHEN Junyu, HUANG Derun, ZHUANG Jieyun*. Validation of qTGW1.1, a Quantitative Trait Locus for 1000grain Weight in Rice (Oryza sativa L.)[J]. Chinese Journal of Rice Science, 2014, 28(2): 125-131.

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水稻千粒重QTL qTGW1.1的验证

Validation of qTGW1.1, a Quantitative Trait Locus for 1000grain Weight in Rice (Oryza sativa L.)

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