中国水稻科学
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中国水稻科学  2011, Vol. 25 Issue (2): 182-188     DOI: 10.3969/j.issn.1001-7216.2011.02.010
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植物激素对杂交稻籽粒灌浆及蔗糖合酶活性的影响
唐瑭1;谢红2;吕冰2 梁建生2,*
(1 淮阴师范学院 生命科学学院, 江苏 淮安 223300; 2 扬州大学 生物科学与技术学院/江苏省作物遗传生理重点实验室, 江苏 扬州 225009; *通讯联系人, E-mail: jsliang@yzu.edu.cn)
Effects of Plant Hormones in Regulating Sucrose Synthase Activity and Grain Filling of Rice
TANG Tang1; XIE Hong2; LU Bing2; LIANG Jian-sheng 2, *
(1 School of Life Sciences, Huaiyin Normal University, Huai’an 223300, China; 2 Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China; *Corresponding author, E-mail: jsliang@yzu.edu.cn)
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摘要 

以亚种间杂交稻两优培九和品种间杂交稻汕优63为材料,对籽粒灌浆期间的内源激素含量和酶活性进行了测定分析,并结合外源激素对水稻籽粒灌浆过程的调控试验,分析了脱落酸对蔗糖代谢关键酶——蔗糖合酶活性的调节作用。结果表明,灌浆前期强势粒中生长素、玉米素核苷和脱落酸(ABA)含量明显高于弱势粒,并与籽粒最大灌浆速率、平均灌浆速率、籽粒充实率和粒重均呈显著或极显著正相关。强势粒中蔗糖合酶活性明显高于弱势粒,且达到峰值的时间也早于弱势粒。籽粒灌浆速率、籽粒充实率、粒重和淀粉积累速率与蔗糖合酶活性呈显著正相关。花后8 d对稻株喷施低浓度ABA显著提高了籽粒中ABA含量和蔗糖合酶活性,增加了粒重和籽粒充实率。喷施ABA合成抑制剂氟草酮作用则相反。上述结果说明,植物激素尤其是ABA对水稻籽粒灌浆有重要的促进作用,其作用在很大程度上是通过调节籽粒蔗糖合酶活性实现的。

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唐瑭
谢红
吕冰
梁建生
关键词脱落酸   籽粒充实   亚种间杂交稻   蔗糖合酶     
Abstract: In order to explore the mechanisms of hormones in the regulation of grain filling, the changes in contents and activities of enzymes which catalyze sucrose metabolism and endogenous hormones in grains during grain filling were analyzed by using inter-subspecies hybrid rice combination Liangyoupeijiu and intervarietal hybrid rice combination Shanyou 63 as materials. Results showed that superior grains had higher contents of endogenous indole-3-acetic acid (IAA), zeatin riboside (ZR) and abscisic acid (ABA) compared with inferior grains at early grain filling stage, and the contents of IAA, ZR and ABA were positively and significantly correlated with the maximum and mean grain filling rate, grain filling percentage, and grain weight. The sucrose synthase (SuSase) activity in superior grains was higher and peaked earlier than that in inferior grains. The grain filling rate, grain filling percentage, grain weight and starch accumulation rate were positively and significantly correlated with the SuSase activity in grains. Application of low concentration of ABA at 8 d after flowering significantly increased endogenous ABA content and SuSase activity in grains, grain filling percentage, and final grain weight. On the contrary, when fluridone (an inhibitor of ABA synthesis) was applied, the ABA content and the activity of SuSase decreased. These results suggest that ABA plays a significant regulatory role in grain filling largely through affecting sucrose synthase activity.


Key wordsabscisic acid   grain filling   inter-subspecies hybrid rice   sucrose synthase   
收稿日期: 2010-05-16;
通讯作者: 梁建生   
引用本文:   
唐瑭,谢红,吕冰等. 植物激素对杂交稻籽粒灌浆及蔗糖合酶活性的影响[J]. 中国水稻科学, 2011, 25(2): 182-188 .
TANG Tang,XIE Hong,LU Bing et al. Effects of Plant Hormones in Regulating Sucrose Synthase Activity and Grain Filling of Rice[J]. , 2011, 25(2): 182-188 .
 
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