In order to preserve virus for identifying resistant rice varieties against rice blackstreaked dwarf disease, a simple and reliable method was developed by which virusfree small brown planthopper(SBPH) acquired rice blackstreaked dwarf virus（RBSDV） from frozen infected leaves and transmitted the virus to healthy rice plants. RBSDV acquired from infected leaves frozen at -70℃ by SBPH was transmitted to a susceptible rice variety. The experimental result showed that the SBPH could acquire the virus from frozen infected leaves and transmit it to the susceptible rice variety. For the ability of obtaining and transmitting the virus by SBPH, there was no significant difference between frozen infected leaves and fresh infected leaves. The novel method could be applied to the resistance identification of rice varieties to rice blackstreaked dwarf disease and it would facilitate the breeding process for rice blackstreaked dwarf disease resistance.
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