基于M1TDS靶向筛选技术的重离子束诱变定向改良杂交水稻卓两优1126性状的研究

韶也, 胡远艺, 彭彦, 毛毕刚, 刘慧敏, 唐婵娟, 雷斌, 唐丽, 余丽霞, 李文建, 罗武中, 罗治斌, 袁远涛, 李曜魁, 张丹, 周利斌, 柏连阳, 唐文帮, 赵炳然

Directed Improvement of Hybrid Rice Zhuoliangyou 1126 by Heavy Ion Beam Mutagenesis Based on M1TDS Targeted Screening Technology

SHAO Ye, HU Yuanyi, PENG Yan, MAO Bigang, LIU Huimin, TANG Chanjuan, LEI Bin, TANG Li, YU Lixia, LI Wenjian, LUO Wuzhong, LUO Zhibin, YUAN Yuantao, LI Yaokui, ZHANG Dan, ZHOU Libin, BAI Lianyang, TANG Wenbang, ZHAO Bingran

图2 M2群体的KASP鉴定及突变单株的Sanger测序验证 A, B：卓234S和湘农恢1126(R1126) M2群体OsNRAMP5基因KASP鉴定及突变单株Sanger测序验证，osnramp5246-8和osnramp537-7分别代表卓234S和湘农恢1126的OsNRAMP5基因突变单株； C, D：卓234S和湘农恢1126 M2群体OsBADH2基因KASP鉴定及Sanger测序验证，osbadh2168-1和 osbadh2224-8分别代表卓234S和湘农恢1126的OsBADH2基因突变单株；E~G：卓234S M2群体OsLOX3、OsPAO5和OsBEⅡb基因KASP鉴定及Sanger测序验证，oslox3760-7、ospao5549-5和osbe2b931-5分别代表卓234S的OsLOX3、OsPAO5和OsBEⅡb基因突变单株。图A中红点代表杂合基因型，蓝点代表纯合突变基因型，绿点代表野生基因型；图B~G中红点代表杂合基因型，绿点代表纯合突变基因型，蓝点代表野生基因型；红色椭圆形框内圆点代表目标基因纯合/杂合突变基因型。

Fig. 2. KASP identification of M2 generation and Sanger sequencing of mutant plants A and B, KASP identification of OsNRAMP5 gene in Zhuo 234S and Xiangnonghui 1126 M2 population and Sanger sequencing of mutants. osnramp5246-8 and osnramp537-7 represent mutant plant of OsNRAMP5 gene in Zhuo 234S and Xiangnonghui 1126, respectively; C and D, KASP identification of OsBADH2 gene in Zhuo 234S and Xiangnonghui 1126 M2 population and Sanger sequencing of mutants; osbadh2168-1and osbadh2224-8 represent mutant plant of OsBADH2 gene in Zhuo234S and Xiang Nonghui 1126, respectively. E-G, KASP identification of OsLOX3, OsPAO5 and OsBEⅡb gene in Zhuo 234S M2 population and Sanger sequencing of mutants. oslox3760-7, ospao5549-5 and osbe2b931-5 represented mutant plant of OsLOX3, OsPAO5 and OsBEⅡb gene of Zhuo 234S, respectively. In panel A, red dots represent heterozygous genotype, blue dots represent homozygous mutant genotype, green dots represent wild genotype. In panels B-G, red dots represent heterozygous genotype, green dots represent homozygous mutant genotype, blue dots represent wild genotype. The dots in the red oval box represent the homozygous/heterozygous genotype of target genes.