基于M1TDS靶向筛选技术的重离子束诱变定向改良杂交水稻卓两优1126性状的研究

韶也, 胡远艺, 彭彦, 毛毕刚, 刘慧敏, 唐婵娟, 雷斌, 唐丽, 余丽霞, 李文建, 罗武中, 罗治斌, 袁远涛, 李曜魁, 张丹, 周利斌, 柏连阳, 唐文帮, 赵炳然

Directed Improvement of Hybrid Rice Zhuoliangyou 1126 by Heavy Ion Beam Mutagenesis Based on M1TDS Targeted Screening Technology

SHAO Ye, HU Yuanyi, PENG Yan, MAO Bigang, LIU Huimin, TANG Chanjuan, LEI Bin, TANG Li, YU Lixia, LI Wenjian, LUO Wuzhong, LUO Zhibin, YUAN Yuantao, LI Yaokui, ZHANG Dan, ZHOU Libin, BAI Lianyang, TANG Wenbang, ZHAO Bingran

图1 目标基因突变混池的KASP基因分型 A~D分别代表OsNRAMP5基因不同突变位点基因分型；E, F 分别代表OsBADH2基因不同突变位点基因分型；G, H分别代表OsLOX3基因不同突变位点基因分型；I~K分别代表OsPAO5基因不同突变位点基因分型；L, M分别代表OsSSIIIa基因和OsBEIIb基因突变位点基因分型；A. 图中246-8代表诱变M1群体246行第8株（下同）；蓝点/绿点代表野生基因型，红点代表突变基因型，灰点代表阴性对照；FAM和HEX分别代表两种不同颜色的荧光修饰标签。

Fig. 1. KASP genotyping in the mixed pool of target gene mutations A-D represent the genotyping of different mutation sites of OsNRAMP5 gene; E and F represent the genotyping of different mutation sites of OsBADH2 gene, respectively; G and H represent genotyping of different mutation sites of OsLOX3 gene, respectively; I-K represent genotyping of different mutation sites of OsPAO5 gene; L, M represent the genotyping of mutation sites in OsSSIIIa gene and OsBEIIb gene, respectively; In panel A, 246-8 represents the 8th plant in 246 rows of M1 population (the same below); Blue/green dots represent wild genotypes, red dots represent mutant genotypes, and gray dots represent negative controls; FAM and HEX represent fluorescently modified labels of two different colors, respectively.