基于M1TDS靶向筛选技术的重离子束诱变定向改良杂交水稻卓两优1126性状的研究

韶也, 胡远艺, 彭彦, 毛毕刚, 刘慧敏, 唐婵娟, 雷斌, 唐丽, 余丽霞, 李文建, 罗武中, 罗治斌, 袁远涛, 李曜魁, 张丹, 周利斌, 柏连阳, 唐文帮, 赵炳然

Directed Improvement of Hybrid Rice Zhuoliangyou 1126 by Heavy Ion Beam Mutagenesis Based on M1TDS Targeted Screening Technology

SHAO Ye, HU Yuanyi, PENG Yan, MAO Bigang, LIU Huimin, TANG Chanjuan, LEI Bin, TANG Li, YU Lixia, LI Wenjian, LUO Wuzhong, LUO Zhibin, YUAN Yuantao, LI Yaokui, ZHANG Dan, ZHOU Libin, BAI Lianyang, TANG Wenbang, ZHAO Bingran

表3 目标基因及靶向测序分析情况

Table 3. Target gene and targeted sequencing analysis

目标基因 Target gene 基因编号 Gene ID 目标表型 Target phenotype 外显子数 No. of exons 目标区域覆盖度 Coverage of target area(%) 平均测序深度 Average sequencing depth OsNRAMP5 Os07g0257200 镉低积累 Low cadmium accumulation 13 100 53398× OsBADH2 Os08g0424500 香味Aroma 15 100 53086× OsLOX3 Os03g0699700 耐储藏Storage-tolerance 9 100 50202× OsPAO5 Os04g0671300 耐淹 Submergence-tolerance 10 100 54263× OsSSIIIa Os08g0191433 低升糖指数 Low glycemic index (GI) 14 100 54510× OsBEIIb Os02g0528200 低升糖指数 Low glycemic index (GI) 22 100 52192×