基于M1TDS靶向筛选技术的重离子束诱变定向改良杂交水稻卓两优1126性状的研究

韶也, 胡远艺, 彭彦, 毛毕刚, 刘慧敏, 唐婵娟, 雷斌, 唐丽, 余丽霞, 李文建, 罗武中, 罗治斌, 袁远涛, 李曜魁, 张丹, 周利斌, 柏连阳, 唐文帮, 赵炳然

Directed Improvement of Hybrid Rice Zhuoliangyou 1126 by Heavy Ion Beam Mutagenesis Based on M1TDS Targeted Screening Technology

SHAO Ye, HU Yuanyi, PENG Yan, MAO Bigang, LIU Huimin, TANG Chanjuan, LEI Bin, TANG Li, YU Lixia, LI Wenjian, LUO Wuzhong, LUO Zhibin, YUAN Yuantao, LI Yaokui, ZHANG Dan, ZHOU Libin, BAI Lianyang, TANG Wenbang, ZHAO Bingran

表2 Sanger测序引物序列

Table 2. Primers for Sanger sequencing

名称 Name 序列 Sequence OsNRAMP5-3E-540F GTTGGTCCTGGATTCATGGTGTC OsNRAMP5-3E-540R TCCAATCAGAATCACCCAGAGCAG OsNRAMP5-1E-265F TCTTCGTCTACTTCCAGCTAGCC OsNRAMP5-1E-265R ACAGTGAACAACTGTCCATGGTG OsBADH-4-471F TTGATGAAGCAGCATGGGACATG OsBADH-4-471R GCTGCTAGGTACAATTTGTGAGAC OsBADH-8-357F CTTCAGCTGCTCCTATGGTTAAGG OsBADH-8-357R TCTAGCATCCAGCTCAGTTAAGTGC OsLOX3-8-535F GAGAAGAACCTCGAAGGCCTCAG OsLOX3-8-535R CCATCACAGCATGTGTGTTGAGC OsPAO-6/7-563F GCTCTCTTTGATAAGGATGGTCGTC OsPAO-6/7-563R ATGGCCACCAGTAAGAACATGTTC OsBEIIb-17-359F GGCATGAGAACCTCACATACTG OsBEIIb-17-359R GGAAGTACTTGTGGAGCTCTTGG