基于M1TDS靶向筛选技术的重离子束诱变定向改良杂交水稻卓两优1126性状的研究

韶也, 胡远艺, 彭彦, 毛毕刚, 刘慧敏, 唐婵娟, 雷斌, 唐丽, 余丽霞, 李文建, 罗武中, 罗治斌, 袁远涛, 李曜魁, 张丹, 周利斌, 柏连阳, 唐文帮, 赵炳然

Directed Improvement of Hybrid Rice Zhuoliangyou 1126 by Heavy Ion Beam Mutagenesis Based on M1TDS Targeted Screening Technology

SHAO Ye, HU Yuanyi, PENG Yan, MAO Bigang, LIU Huimin, TANG Chanjuan, LEI Bin, TANG Li, YU Lixia, LI Wenjian, LUO Wuzhong, LUO Zhibin, YUAN Yuantao, LI Yaokui, ZHANG Dan, ZHOU Libin, BAI Lianyang, TANG Wenbang, ZHAO Bingran

表1 KASP基因分型引物序列

Table 1. Primer sequence for KASP genotyping

名称 Name 序列 Sequence OsNRAMP5-246-8-F GAAGGTGACCAAGTTCATGCTTGTGGTTGGCTCCGGCTTAA OsNRAMP5-246-8-H GAAGGTCGGAGTCAACGGATTTGTGGTTGGCTCCGGCTTGC OsNRAMP5-246-8-C CACAAAATGAAACAGTGGAAACCGATC OsNRAMP5-234-6-F GAAGGTGACCAAGTTCATGCTCACAAAATGAAACAGTGGAAACCGAT OsNRAMP5-234-6-H GAAGGTCGGAGTCAACGGATTCACAAAATGAAACAGTGGAAACCGAC OsNRAMP5-234-6-C CGTACCTCATATCTGTGGTTGGCTC OsNRAMP5-204-6-F GAAGGTGACCAAGTTCATGCTCACAAAATGAAACAGTGGAAACCGA OsNRAMP5-204-6-H GAAGGTCGGAGTCAACGGATTCACAAAATGAAACAGTGGAAACCGC OsNRAMP5-204-6-C CGTACCTCATATCTGTGGTTGGCTC OsNRAMP5-37-7-F GAAGGTGACCAAGTTCATGCTTGAAGAAGCTAAGAGAGGAAGCAACAA OsNRAMP5-37-7-H GAAGGTCGGAGTCAACGGATTTGAAGAAGCTAAGAGAGGAAGCAACAG OsNRAMP5-37-7-C ACTAGCTCTCCAGCTGATGCTC OsBADH2-168-1-F GAAGGTGACCAAGTTCATGCTGTGTAGTTGGGTTGATCACACCTT OsBADH2-168-1-H GAAGGTCGGAGTCAACGGATTGTGTAGTTGGGTTGATCACACCTC OsBADH2-168-1-C TGGGTCATAAATAAATATAAGCGCAGG OsBADH2-224-8-F GAAGGTGACCAAGTTCATGCTTTCGTCTTTTCTTGACAGCCTGTT OsBADH2-224-8-H GAAGGTCGGAGTCAACGGATTTTCGTCTTTTCTTGACAGCCTGTC OsBADH2-224-8-C AGGACTTTTTCCACCAAGTTCCAG OsLOX3-760-7-F GAAGGTGACCAAGTTCATGCTTATCTTCACCTATGCCACAAGGC OsLOX3-760-7-H GAAGGTCGGAGTCAACGGATTTATCTTCACCTATGCCACAAGGA OsLOX3-760-7-C CAGGGTATCATCATCACGTAAGAAC OsLOX3-115-5-F GAAGGTGACCAAGTTCATGCTGAGAGCAAAGTGTTGAACATGAAC OsLOX3-115-5-H GAAGGTCGGAGTCAACGGATTGAGAGCAAAGTGTTGAACATGAAG OsLOX3-115-5-C GGACCGACCCGGTTCTTGAG OsPAO5-549-5-F GAAGGTGACCAAGTTCATGCTTGCTTGACAGGAATCCACACCTA OsPAO5-549-5-H GAAGGTCGGAGTCAACGGATTTGCTTGACAGGAATCCACACCTG OsPAO5-549-5-C GCACCACCATTCCAGATATAGGTG OsPAO5-1028-4-F GAAGGTGACCAAGTTCATGCTAACTTTCCTCCAGGGTTACCAAAAT OsPAO5-1028-4-H GAAGGTCGGAGTCAACGGATTAACTTTCCTCCAGGGTTACCAAATC OsPAO5-1028-4-C GCTTGTCCCATCTTCAACACATAC OsPAO5-84-2-F GAAGGTGACCAAGTTCATGCTCTCAGCTCAAGAAAATGCTACCAGG OsPAO5-84-2-H GAAGGTCGGAGTCAACGGATTCTCAGCTCAAGAAAATGCTACCACT OsPAO5-84-2-C GACAGTACTCAACTGTACATGGTCTG OsSSIIIa-598-7-F GAAGGTGACCAAGTTCATGCTCCATTTGGTTCAAGGCCTAGAACTC OsSSIIIa-598-7-H GAAGGTCGGAGTCAACGGATTCCATTTGGTTCAAGGCCTAGAACTG OsSSIIIa-598-7-C CACTGTTTTCGACGTAGACCATG OsBEIIb-931-5-F GAAGGTGACCAAGTTCATGCTGTGTGATTGTTATTAAATCTCACCAGGA OsBEIIb-931-5-H GAAGGTCGGAGTCAACGGATTGTGTGATTGTTATTAAATCTCACCAGGC OsBEIIb-931-5-C GGCTATCTTAACTTTATGGGAAATGAGTTC