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    Research Progress of Pollen Tube Growth in Pistil of Rice and Its Response to Abiotic stress
    XU Yongqiang, XU Jun, FENG Baohua, XIAO Jingjing, WANG Danying, ZENG Yuxiang, FU Guanfu
    Chinese Journal OF Rice Science    2024, 38 (5): 495-506.   DOI: 10.16819/j.1001-7216.2024.231107
    Abstract711)   HTML25)    PDF (618KB)(6250)       Save

    Rice, a vital food crop in China and globally, is susceptible to abiotic stresses such as extreme high temperatures, drought, cold, and low light which can impair rice production and threaten worldwide food security, including in China. The growth of pollen tubes in the pistil is crucial for successful fertilization, however abiotic stress can disrupt signaling transport and energy metabolism in the pistils, leading to spikelet sterility. Therefore, it is essential to investigate the impact of abiotic stress on pollen tube growth in pistils and to understand the underlying regulatory mechanisms. This review focuses on the process of pollen tube growth in pistils, encompassing signal transduction, and energy metabolism. Furthermore, it explores the effects of abiotic stress on pollen tube growth in pistils and describes strategies to mitigate stress-induced inhibition of pollen tube growth. This research provides a theoretical foundation for developing cultivation techniques aimed at enhancing spikelet fertility in rice under abiotic stress. Finally, the paper outlines future research directions for studying pollen tube growth in rice pistils under abiotic stress.

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    Identification of Candidate Genes for Rice Nitrogen Use Efficiency by Genome-wide Association Analysis
    LÜ Yang, LIU Congcong, YANG Longbo, CAO Xinglan, WANG Yueying, TONG Yi, Mohamed Hazman, QIAN Qian, SHANG Lianguang, GUO Longbiao
    Chinese Journal OF Rice Science    2024, 38 (5): 516-524.   DOI: 10.16819/j.1001-7216.2024.231010
    Abstract1135)   HTML29)    PDF (2255KB)(6050)       Save

    Objective】 The exploration of germplasm and gene resources in rice for high nitrogen efficiency, along with the elucidation of their molecular mechanisms and genetic effects, represents a significant focus and goal within current research efforts on rice nitrogen use efficiency (NUE).【Method】 To identify the variant loci and candidate genes associated with rice NUE, we collected 190 Asian rice accessions as an association population. After thorough filtering and screening, we obtained 3,934,195 high-quality single nucleotide polymorphisms (SNPs). Under field conditions, two nitrogen treatment levels were established: low nitrogen (N1, 90 kg/hm²) and normal nitrogen (N2, 180 kg/hm²). We investigated the phenotypic data of rice leaf width in response to both low and normal nitrogen treatments at the maturity stage. Genome-wide association analysis (GWAS) was conducted by integrating the FarmCPU and MLM models. 【Result】By calculating the phenotypic ratio Q (N1/N2) of leaf width under low and normal nitrogen levels, we found that the Q value exhibited a normal distribution. A total of 100 significant loci were identified on 12 chromosomes through GWAS for the Q value, leading to the determination of 39 candidate QTLs. This included the cloned NUE-related genes OsNR1.2 and OsNAC42. Additionally, we identified superior haplotypes and potential advantageous haplotype combinations of the candidate genes OsNR1.2 and OsNAC42, which provide valuable resources and information for enhancing rice NUE. 【Conclusion】This study elucidated the genetic basis of rice leaf width under varying nitrogen treatments using GWAS and haplotype analysis. We identified candidate QTLs and genes associated with NUE, including OsNR1.2 and OsNAC42. Through haplotype analysis, we recognized advantageous haplotype combinations of these two genes, offering valuable resources and insights for the improvement of rice NUE.

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    Research Progress in Regulation of Anthocyanin Accumulation in Colored Rice
    WU Wanting, XU Qian, LIU Dantong, ZHU Changjin, DU Haotian, JU Haoran, HUO Zhongyang, DAI Qigen, LI Guohui, XU Ke
    Chinese Journal OF Rice Science    2025, 39 (5): 601-614.   DOI: 10.16819/j.1001-7216.2025.241206
    Abstract519)   HTML22)    PDF (1641KB)(5435)       Save

    Colored rice is rich in protein, amino acids, cellulose, and essential minerals for the human body. It also contains active substances such as anthocyanins, which have anti-aging, cancer prevention, and antiviral effects, making it widely valued by consumers. This review focuses on summarizing the nutritional and health value of colored rice, and summarizes the accumulation, biosynthesis, and regulation processes of anthocyanins in colored rice from the perspectives of physiological and molecular regulation, as well as the effects of cultivation conditions such as temperature, light, water, and fertilizer level on the accumulation of anthocyanins. Finally, future research directions for colored rice are discussed.

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    Research Progress in Rice Quality of Ratoon Rice
    XIAO Wuwei, ZHU Chenguang, WANG Fei, XIONG Dongliang, HUANG Jianliang, PENG Shaobing, CUI Kehui
    Chinese Journal OF Rice Science    2025, 39 (1): 33-46.   DOI: 10.16819/j.1001-7216.2025.240412
    Abstract1469)   HTML23)    PDF (1363KB)(4947)       Save

    Ratoon rice is a green and efficient planting method that is significant for enhancing the profitability of rice cultivation and ensuring food security in China. Due to differences in growth environments and cultivation management practices, there are notable variations in rice quality between the main season and the ratoon season. Typically, the quality of rice in the ratoon season is superior to that of the main season. Factors such as ratooning characteristics, environmental temperature, and water and fertilizer management can influence rice quality by regulating source-sink relationships, the grain filling process, the synthesis of grain nutrients, and the physicochemical characteristics of starch granules. Numerous high-quality and high-yield rice varieties have been identified as suitable for ratoon rice cultivation, considering their ratooning capacity and yields. However, the quality of rice in both the main and ratoon seasons remains a limiting factor for ratoon rice production. This work briefly introduces the rice quality characteristics of the main and ratoon seasons and reviews the factors affecting the quality of ratoon rice and its cultivation regulations in terms of the formation process of rice quality. This review may lay a theoretical basis for the production of high-quality and high-yield ratoon rice.

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    Research Advances of Fragrance Substances in Rice
    ZHANG Laitong, YANG Le, LIU Hong, ZHAO Xueming, CHENG Tao, XU Zhenjiang
    Chinese Journal OF Rice Science    2025, 39 (2): 171-186.   DOI: 10.16819/j.1001-7216.2025.240301
    Abstract1145)   HTML49)    PDF (1042KB)(4594)       Save

    The distinctive fragrance of rice is regarded as an invaluable trait, and fragrant rice commands a premium price in the market and is highly favored by consumers. The production of flavor is associated with mutations in the Bahd2 gene, which results in the inactivation of betaine aldehyde dehydrogenase, leading to an increase in the 2-AP precursor substance and ultimately to the accumulation of 2-AP and the formation of flavor. Nevertheless, the genetic background of flavor production in rice and the physiological and biochemical mechanisms of flavor substance formation remain incompletely elucidated. This paper presents a summary of the genetic background, gene function, and synthesis pathway of fragrance substances that have been identified thus far. Furthermore, the status of rice breeding in recent years and the response of fragrant rice under different environmental conditions and cultivation techniques are comprehensively sorted out and summarized. This is done with the objective of providing a scientific reference for breeding new fragrant rice varieties.

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    Allele Mining and Breeding Application of Grain Number per Panicle Gene Gn1a in Rice
    LIU Zhichao, CHANG Longxue, AI Xin, JIN Long, ZHANG Fengyong, LI Zhiyong, WANG Yifeng, TONG Xiaohong, HUANG Jie, ZHANG Jian, JIN Jian, YING Jiezheng
    Chinese Journal OF Rice Science    2025, 39 (2): 220-230.   DOI: 10.16819/j.1001-7216.2025.240105
    Abstract704)   HTML44)    PDF (6499KB)(4283)       Save

    【Objective】Based on advances in rice functional genomics, this study aimed to screen favorable alleles of the Gn1a gene controlling grain number per panicle, providing technical support for modern rice variety improvement. 【Method】Advanced genetic populations were developed through backcrossing and self-crossing for QTL mapping. Near-isogenic lines (NILs) were constructed to validate QTL effects. Target gene sequences were amplified and aligned, followed by designing functional markers for genotyping. 【Result】A major quantitative trait locus (QTL) controlling secondary branch number and grains per panicle was co-localized with the Gn1a locus on chromosome 1 short arm. This mapping utilized four BC3F3 populations derived from crosses between the japonica donor parent Jizi 1560 and recurrent parents, including early indica varieties (Zhongjiazao 17, Zhongzao 39) and three-line restorer lines (Huazhan, R173). Sequence analysis revealed an unreported indica-specific allele (Gn1a-i) in Zhongjiazao 17 and Zhongzao 39, characterized by a ‌16-bp deletion in the 5' UTR‌ compared to the japonica allele (Gn1a-j). Comparative analyses of four near-isogenic line (NIL) populations confirmed that Gn1a-i significantly increased grains per panicle and yield. A functional marker (ZC51) targeting the 16-bp deletion was developed to genotype 39 rice materials, which were categorized into nine types: conventional early/late indica varieties, three-line maintainers/restorers, two-line sterile lines, northern/southern japonica varieties, landraces, and wild rice. All modern indica materials (cultivars and hybrid parents) carried Gn1a-i, whereas landraces and wild rice retained Gn1a-j. Notably, both southern and northern japonica varieties exhibited biallelic Gn1a-i and Gn1a-j‌. 【Conclusion】It was confirmed that Gn1a-i allele increased grain yield by increasing number of primary branches, secondary branches and grains per panicle. The favorable allele of Gn1a-i has great potentials in the improvement of japonica rice varieties, and the functional gene marker of ZC51 can be directly used in the marker-assisted selection of Gn1a.

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    Advances in Roles of Different Types of Histone Modifications in Responses of Rice to Abiotic Stresses
    HAO Wenqian, CAI Xingjing, YANG Haidong, WU Yuyang, TENG Xuan, XUE Chao, GONG Zhiyun
    Chinese Journal OF Rice Science    2025, 39 (5): 575-585.   DOI: 10.16819/j.1001-7216.2025.250203
    Abstract567)   HTML34)    PDF (1171KB)(3769)       Save

    As one of the most important food crops in the world, the yield and quality of rice are directly related to global food security. However, rice often faces various abiotic stresses during growth, such as salt stress, drought, low and high temperatures, which seriously compromise the growth, development and yield of rice. In recent years, epigenetics research, especially the role of histone modifications in regulating stress tolerance responses of rice, has attracted increasing attention. This review summarizes the latest research progress in histone modifications in abiotic stress responses in rice, to provide a theoretical basis for genetic improvement and stress resistance breeding of rice.

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    Research Progress in Influencing Factors of Methane Production and Emission as Well as Emission Reduction Measures in Paddy Fields
    XIE Xianzhi, LIU Qihua, LI Xinhua, LI Weiping
    Chinese Journal OF Rice Science    2024, 38 (5): 475-494.   DOI: 10.16819/j.1001-7216.2024.231102
    Abstract1049)   HTML35)    PDF (1684KB)(3739)       Save

    The emission of methane from paddy fields is regarded as one of the most significant sources of carbon emissions in agricultural production. Consequently, modern agricultural researchers are focusing on how to effectively reduce methane emissions without sacrificing grain yield in rice production. This review summarizes recent advances in understanding methane production, emission, and strategies for reducing emissions in paddy fields, based on current research findings. It clarifies the processes of methane production, oxidation, and emission, as well as the key influencing factors. The production and emission of methane are closely correlated with the physicochemical properties of soil (including pH, key enzyme activities, water content, temperature, oxidation-reduction potential, and texture), rice varieties (including root morphology and activity, root exudation, aerenchyma, harvest index, and plant height), cultivation techniques (including crop rotation patterns, irrigation and fertilization management practices, and planting methods), and climate factors (including air temperature, atmospheric carbon dioxide concentration, and UV-B radiation). In this review, management practices aiming at decreasing methane emissions are summarized from the perspectives of new variety breeding and comprehensive regulation methods: 1) Selecting evaluation parameters based on the differences in methane emission levels among various rice varieties and breeding new rice varieties that achieve high grain yield with low methane emissions; 2) Exploring new substances that can reduce methane emissions in paddy fields; 3) Investigating innovative technologies for low-carbon cycled cultivation and breeding of rice and fish systems that promote low-carbon circulation, enhance carbon fixation capacity, and reduce methane emissions in paddy soil, as well as the integration of multiple cultivation methods. Additionally, we should explore the pathways for reducing methane emissions in paddy fields according to different production environments in rice-growing regions and establish a high-efficiency comprehensive cultivation regulation system. Reasonable suggestions for future research directions regarding the reduction of methane emissions in paddy fields are presented. It is hoped that this paper will provide not only theoretical evidence for high-yield rice cultivation with lower methane emissions but also technical references for achieving the national "dual carbon" goals.

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    Sterility Mechanism of Photoperiod/Thermo-sensitive Genic Male Sterile Lines and Development and Prospects of Two-line Hybrid Rice
    WANG Yichen, ZHU Benshun, ZHOU Lei, ZHU Jun, YANG Zhongnan
    Chinese Journal OF Rice Science    2024, 38 (5): 463-474.   DOI: 10.16819/j.1001-7216.2024.231008
    Abstract1192)   HTML30)    PDF (1010KB)(3116)       Save

    This review documented the research progress of two-line hybrid rice in China, and introduced germplasm resources of photoperiod/thermo-sensitive genic male sterile (P/TGMS) lines in two-line hybrid rice breeding. We also summarized various genetic and cellular mechanisms of P/TGMS in plants, and suggestions were proposed for the genetic resource optimization and breeding strategies of P/TGMS lines in two-line hybrid rice breeding.

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    Development and Application of Specific Molecular Markers for Eight Rice Blast Resistance Genes in Rice
    YANG Hao, HUANG Yanyan, WANG Jian, YI Chunlin, SHI Jun, TAN Chutian, REN Wenrui, WANG Wenming
    Chinese Journal OF Rice Science    2024, 38 (5): 525-534.   DOI: 10.16819/j.1001-7216.2024.230811
    Abstract805)   HTML25)    PDF (2859KB)(3045)       Save

    Objective】To elucidate the composition of blast resistance genes in rice parents and facilitate their effective utilization in rice resistance breeding, it’s crucial to develop molecular markers that transcend genetic background limitations. These molecular markers can accurately discern resistance genes in parents, serving as essential tools for marker-assisted breeding.【Method】By comparing the coding sequence of each Resistance gene with allelic genes in 155 rice resources, nucleotide polymorphism sites within each resistance gene were identified. Primer pairs for molecular markers were then developed targeting the most specific polymorphism sites.【Results】Effective molecular markers were successfully developed for eight resistance genes: Pit, Pish, Pib, Pid3, Pi5, Pia, Pi54, and Pita2/Ptr. These markers were validated through examination using multiple positive and negative controls or gene sequencing. Subsequently, these markers were utilized to identify the resistance gene composition in 109 breeding parent lines commonly used in the Sichuan Basin. Results revealed that the Pia gene was absent in these parent lines, while Pit, Pish, Pi54, Pid3, Ptr/Pita2, Pi5, and Pib genes were present in 3.67%, 13.76%, 14.68%, 18.35%, 24.77%, 26.61%, and 38.53% of lines, respectively. Furthermore, 21.10% of the rice parent lines lacked these resistance genes, 35.78% possessed one resistance gene, and 43.12% harbored two to four resistance genes.

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    Development and Application of Functional Markers for Purity Identification of Wild-abortive Three-line Hybrid Rice
    ZHANG Mengke, LU Jiayu, HE Jin, XU Xue, WU Shuang, WANG Peiran, CHEN Ruofan, JIN Qing, WANG Xiufeng
    Chinese Journal OF Rice Science    2026, 40 (2): 244-252.   DOI: 10.16819/j.1001-7216.2026.250204
    Abstract190)   HTML10)    PDF (1575KB)(2570)       Save

    【Objective】 Developing stable, efficient, and reliable markers for purity testing of three-line hybrid seeds is crucial for current hybrid rice seed production. 【Method】 Based on the specific sequences of the wild abortive-type sterility gene WA352, the major fertility-restoring gene Rf4, and the internal reference gene Actin, multiple single-gene detection markers were designed. PCR amplification was then performed by optimizing the primer mixture ratio. 【Result】 A mixture of primers WA352-2, Rf4-3, and Actin-3 in a ratio of 1:11:3 enabled simultaneous detection of the genotypes of WA352, Rf4, and Actin in a single PCR reaction, allowing differentiation among wild abortive-type CMS lines, restorer lines, maintainer lines, and hybrids. 【Conclusion】 The detection method established in this study is simple to perform and yields stable and reliable results. It can not only identify genotypes in wild-abortive three-line rice but also serve as an effective tool for molecular marker-assisted breeding. By improving the accuracy of purity assessment during breeding, this method provides strong support for achieving high-yield and high-quality rice breeding objectives in China.

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    Transcriptome Analysis of Top Second Leaf Sheath of Rice Under Different Nitrogen Fertilizer Levels
    HUANG Fudeng, WU Chunyan, HAO Yuanyuan, HAN Yifei, ZHANG Xiaobin, SUN Huifeng, PAN Gang
    Chinese Journal OF Rice Science    2025, 39 (4): 563-574.   DOI: 10.16819/j.1001-7216.2025.250306
    Abstract395)   HTML15)    PDF (2200KB)(2467)       Save

    【Objective】 The leaf sheath is an important organ that simultaneously serves the functions of “source, sink, and transport”, playing a crucial role in the growth and development of rice plants. Identifying important genes in leaf sheaths in response to nitrogen will provide scientific evidence for the genetic improvement of nitrogen use efficiency in rice. 【Method】 The japonica rice cultivar Xiushui 134 was planted in percolation ponds at low nitrogen (LN), medium nitrogen (MN), and high nitrogen (HN) levels. The top second leaf sheaths were collected on the 10th day after heading for transcriptome sequencing analysis to identify important genes involved in the nitrogen response of rice leaf sheaths. 【Result】 Comparative analysis of the transcriptome data revealed 1,791 differentially expressed genes at the three nitrogen levels. The number of upregulated and downregulated genes between HN and MN, MN and LN, and HN and LN were 312 and 155, 263 and 160, and 1,059 and 542, respectively. The common upregulated and downregulated genes at the three nitrogen levels were 15 and 53, respectively, involved in physiological and biochemical processes such as nutrient element absorption and transport, responses to biotic and abiotic stresses, plant hormone responses, and light regulation. Based on GO enrichment analysis, KEGG metabolic pathway analysis, a total of 6 known functional genes related to nitrogen utilization and 4 genes related to photosynthesis, as well as 50 genes of unknown function, were identified. It is speculated that these genes play important roles in the nitrogen response of the second leaf sheath in rice. 【Conclusion】 The research results preliminarily clarify the important genes involved in the leaf sheath's response to different nitrogen levels, which may play significant roles in signal transduction pathways of nitrogen utilization.

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    Research Progress in Mining and Utilization of Rice Blast Resistance Genes
    YANG Jie, YANG Changdeng, ZENG Yuxiang, HOU Yuxuan, CHEN Tianxiao, LIANG Yan
    Chinese Journal OF Rice Science    2024, 38 (6): 591-603.   DOI: 10.16819/j.1001-7216.2024.230911
    Abstract1896)   HTML80)    PDF (430KB)(2402)       Save

    Rice blast is a disease caused by Magnaporthe oryzae, which seriously threatens the yield and quality of rice. At present, breeding disease-resistant varieties is the most economical and effective way to control rice blast, and identifying resistance genes is the key to disease-resistant variety breeding. The review summarized the types of rice blast resistance genes cloned as well as the current mainstream techniques for identifying blast-resistant genes. The application of these genes has been outlined along with future development directions.

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    Directed Improvement of Hybrid Rice Zhuoliangyou 1126 by Heavy Ion Beam Mutagenesis Based on M1TDS Targeted Screening Technology
    SHAO Ye, HU Yuanyi, PENG Yan, MAO Bigang, LIU Huimin, TANG Chanjuan, LEI Bin, TANG Li, YU Lixia, LI Wenjian, LUO Wuzhong, LUO Zhibin, YUAN Yuantao, LI Yaokui, ZHANG Dan, ZHOU Libin, BAI Lianyang, TANG Wenbang, ZHAO Bingran
    Chinese Journal OF Rice Science    2025, 39 (5): 624-634.   DOI: 10.16819/j.1001-7216.2025.240106
    Abstract516)   HTML11)    PDF (2114KB)(2351)       Save

    Objective】Using the two-line hybrid rice Zhuoliangyou 1126 as the base variety, we aimed to rapidly develop upgraded varieties with traits such as low cadmium accumulation, fragrance, storage tolerance, submergence tolerance, and a low glycemic index.【Method】Zhuo 234S and Xiangnonghui 1126, the parental lines of Zhuoliangyou 1126, were mutagenized using heavy-ion beams. Chimeric mutants of the OsNRAMP5, OsBADH2, OsLOX3, OsPAO5, OsSSIIIa, and OsBEIIb genes were identified in the M1 generation via M1TDS technology. Mutations in the target genes were further detected and isolated in the M2 generation using Kompetitive Allele-Specific PCR (KASP) genotyping. Hybrid rice with low cadmium accumulation was developed by crossing parents carrying OsNRAMP5 mutations.【Results】A total of 13 chimeric mutants involving six target genes were identified in the M1 generation. Among these, seven were confirmed to carry homozygous or heterozygous mutations in the M2 generation. The cadmium content in OsNRAMP5 homozygous mutants was significantly lower than in the wild type, while the content of the aromatic compound 2-acetylpyrroline (2-AP) was significantly higher in OsBADH2 homozygous mutants. When the low-cadmium hybrid rice derived from OsNRAMP5-mutated parents was pot-grown in cadmium-contaminated soil (available Cd: 0.677 mg/kg, pH 5.6), the cadmium content in grains remained consistently below 0.05 mg/kg, compared to 0.91 mg/kg in the wild-type control.【Conclusion】The combination of heavy-ion beam mutagenesis and M1TDS technology enabled the rapid improvement of traits such as low cadmium accumulation in Zhuoliangyou 1126. This study provides a successful example and a general technical reference for transitioning from traditional mutagenesis breeding to directed mutagenesis breeding.

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    Sequence Variation Analysis of the Parthenogeny-inducing Gene BBM1 in Rice
    WANG Qing, WANG Yanru, ZHANG Xiuli, LÜ Qiming
    Chinese Journal OF Rice Science    2024, 38 (6): 627-637.   DOI: 10.16819/j.1001-7216.2024.231210
    Abstract522)   HTML10)    PDF (2276KB)(2327)       Save

    【Objective】Ectopic expression of BBM1 in rice egg cells can induce parthenogenesis. Exploring superior allelic variants of BBM1 and functionally similar genes is of great significance for heterosis fixation in rice.【Method】By utilizing third-generation sequencing data from 383 core rice accessions, we conducted an analysis of structural variations, sequence polymorphisms, and haplotypes of BBM1. Additionally, we screened for genes with similar sequences and expression patterns to identify functional analogues of BBM1.【Results】Our findings revealed the presence of two copies of the BBM1 gene in three rice accessions, along with MITEs insertion variants observed in two O. rufipogon accessions. The BBM1 sequence exhibited a clear indica-japonica differentiation, with 94.80% of japonica accessions and 3.80% of indica accessions experiencing premature termination due to single base mutations, resulting in the loss of 26 amino acid residues. Furthermore, the coding region of BBM1 is highly conserved, and most rice accessions share the same haplotype. We also identified LOC_Os06g44750 as a candidate gene with a similar sequence and expression pattern to BBM1, which may potentially contribute to inducing parthenogenesis.【Conclusion】This study provides insights into the variation present within the BBM1 gene in rice while also identifying important haplotypes and potential candidate genes with functional similarities. This foundational knowledge serves to enhance the frequency of parthenogenesis in rice.

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    Research Progress in Rice Mesocotyl
    REN Ningning, SUN Yongjian, SHEN Congcong, ZHU Shuangbing, LI Huiju, ZHANG Zhiyuan, CHEN Kai
    Chinese Journal OF Rice Science    2025, 39 (1): 11-23.   DOI: 10.16819/j.1001-7216.2025.240504
    Abstract1060)   HTML24)    PDF (1979KB)(2186)       Save

    As one of the most important food crops in the world, the growth and development characteristics of rice significantly impact agricultural production. The mesocotyl is a crucial part of rice that connects seeds and seedlings, playing an essential role in the processes of seed germination and emergence. Its elongation characteristics are particularly important in the direct seeding of rice. The elongation performance of the mesocotyl directly affects the emergence rate, emergence speed, and stress resistance of rice seedlings, making it a key link in rice breeding and cultivation management. With the advancement of modern agricultural technology, especially the rise of rice direct seeding technology, research on rice mesocotyl has become increasingly urgent and important. This manuscript reviews the cell morphology, environmental factors, physiological factors, mesocotyl-related QTLs, gene mining, and their applications in breeding. The goal is to lay a theoretical basis and explore new strategies for the utilization of rice mesocotyl and to guide future breeding directions.

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    Mechanisms of Arsenic Uptake and Transport in Rice and Agronomic Mitigation Strategies
    WU Jinshui, TANG Jiangying, TAN Li, GUO Zhiqiang, YANG Juan, ZHANG Xinzhen, CHEN Guifang, WANG Jianlong, SHI Wanju
    Chinese Journal OF Rice Science    2025, 39 (2): 143-155.   DOI: 10.16819/j.1001-7216.2025.240402
    Abstract1087)   HTML70)    PDF (1329KB)(2176)       Save

    In recent years, the issue of arsenic contamination in soils has become increasingly severe. Arsenic not only negatively impacts the yield and quality of rice, but also enters the human body through the food chain, posing a serious threat to human health. Therefore, reducing arsenic accumulation in rice has become an urgent problem in order to ensure food safety and promote the development of the rice industry. In-depth research into the mechanisms of arsenic uptake, translocation, and accumulation in rice, along with the active exploration of agronomic cultivation measures to reduce arsenic accumulation, represents an effective approach to addressing excessive arsenic levels in rice grains. This paper provides an overview of the physiological and molecular mechanisms underlying the uptake of different forms of arsenic from soil by rice roots, as well as its translocation and accumulation within rice plants. Factors influencing arsenic uptake and accumulation in rice, such as rice varieties, soil physicochemical properties, soil nutrients, and microorganisms, are discussed. Additionally, the regulatory effects and mechanisms of cultivation practices, particularly water and nutrient management, on reducing arsenic uptake and accumulation in rice are highlighted. Building on existing research findings, future directions for arsenic pollution prevention and control in rice are proposed, aiming to provide a reference for studies focused on reducing arsenic content in rice grains.

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    Assembly, Codon Usage Bias, and Phylogenetic Analysis of Chloroplast Genome of Oryza meyeriana
    LI Rongxin, WANG Bo, XIAO Suqin, YIN Fuyou, ZHANG Jianhong, ZHONG Qiaofang, CHEN Ling, LI Jinlu, YANG Hesheng, CHENG Zaiquan, LIU Li
    Chinese Journal OF Rice Science    2025, 39 (6): 801-812.   DOI: 10.16819/j.1001-7216.2025.240612
    Abstract283)   HTML7)    PDF (2031KB)(2141)       Save

    【Objective】 Oryza meyeriana, which is shade-tolerant and retains many primitive traits, is the only xerophytic type of wild rice in the genus Oryza. This study aims to enrich the genetic foundation of O. meyeriana and provide reference for vector construction of its superior genes. 【Methods】 The chloroplast genome of Yunnan O. meyeriana was sequenced using second-generation sequencing technology, followed by assembly and annotation. Codon usage bias and phylogenetic relationships were analyzed with RStudio and MEGA. 【Results】 The results showed that the total length of the chloroplast genome was 135,937 bp, with a GC content of 39.0%. The average effective number of codons(ENC) value was 48.09, indicating weak codon usage bias. ENC-plot, PR2-plot, and neutral plot analyses revealed that selection pressure plays a major role in shaping the chloroplast codon usage bias in Oryza meyeriana, and that this bias mainly regulates genes involved in self-replication and the photosynthetic system. A total of 16 optimal codons were identified, among which 14 ended with A/U and 2 ended with G/C. Phylogenetic analysis based on the chloroplast genome showed that Oryza meyeriana is closely related to O. brachyantha.【Conclusion】 This study enriches our understanding of the genomic composition and genetic information of Oryza meyeriana. The findings provide a basis for mining favorable genes related to photosynthesis and for investigating the evolution of wild rice species.

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    Development Status and Regional Differences of japonica Rice Quality in the Yangtze River Delta Region from 2002 to 2022
    SHAO Yafang, ZHU Dawei, ZHENG Xin, MOU Renxiang, ZHANG Linping, CHEN Mingxue
    Chinese Journal OF Rice Science    2025, 39 (2): 264-276.   DOI: 10.16819/j.1001-7216.2025.240408
    Abstract497)   HTML12)    PDF (9436KB)(2034)       Save

    【Objective】The aim of this study is to analyze and understand the development status of japonica rice quality in the Yangtze River Delta region of China, as well as the differences of rice qualities among Anhui, Jiangsu and Zhejiang provinces. 【Method】After collecting a total of 11 310 japonica rice varieties grown in Jiangsu, Anhui, and Zhejiang provinces between 2002 and 2022, grain length, length-to-width ratio, brown rice rate, milled rice rate, head rice rate, chalkiness, chalky grain rate, transparency, apparent amylose content, gel consistency, alkali spreading value and protein content were analyzed. Principal component analysis was carried out to explore the quality characteristics of japonica rice cultivated in Anhui, Jiangsu and Zhejiang provinces. 【Results】The results revealed that the grain shape and head rice rate of japonica rice planted in Jiangsu Province were relatively stable during 2002−2022. The chalkiness level fluctuated between 3.06% and 4.93% from 2002 to 2019, and their average value and variation range decreased significantly from 2020 to 2022. The average apparent amylose content in 2012 and 2013 decreased by approximately 2% than the last year, and eventually stabilized between 13.2% and 14.8%. In Zhejiang Province, the grain shape of japonica rice gradually shifted towards longer grains since 2012. The head rice rate exhibited a zigzag pattern, with higher annual averages occurring between 2011 and 2014, and lowers between 2018 and 2022. The chalkiness level showed a zigzag fluctuation from 2002 to 2006, peaked between 2007 and 2015, and then gradually declined in 2016−2018 and 2019−2022. Except for the period from 2013 to 2017, the average apparent amylose content fluctuated between 15.3% and 16.8%, and japonica rice varieties with lower apparent amylose content emerged since 2016. In Anhui Province, the grain shape of japonica rice began to shift towards longer grains from 2020. The decline in head rice rate could be clearly divided into three stages: 2003−2009, 2010−2014, and 2018−2022. The chalkiness level initially rose and then fell, peaking between 2008 and 2014. The apparent amylose content undergone two distinct stages: a period of significant fluctuations from 2002 to 2012, followed by a phase of minor fluctuations and stabilization from 2013 to 2022. The high quality rate of transparency exceeded 80% in most years across all three provinces. Other indicators such as protein content, brown rice rate, milled rice rate, alkali spreading value, and gel consistency did not show significant differences among japonica rice varieties cultivated in the Yangtze River Delta region. 【Conclusions】The quality of japonica rice in the Yangtze River Delta is gradually improving in appearance and palatability. The japonica rice in Jiangsu Province had shorter grain length, lower apparent amylose content and longer gel consistency with excellent processing and appearance qualities. The japonica rice in Zhejiang Province had poor appearance and processing quality, with the grain shape gradually becoming longer and gel consistency and alkali spreading value being relatively small. Anhui Province is intermediate between Jiangsu and Zhejiang provinces in grain shape and other quality traits.

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    Research Progress in Rice Yield Estimation Method Based on Low-altitude Unmanned Aerial Vehicle Remote Sensing
    FENG Xiangqian, WANG Aidong, HONG Weiyuan, LI Ziqiu, QIN Jinhua, ZHAN Lichuan, CHEN Lipeng, ZHANG Yunbo, WANG Danying, CHEN Song
    Chinese Journal OF Rice Science    2024, 38 (6): 604-616.   DOI: 10.16819/j.1001-7216.2024.240103
    Abstract921)   HTML36)    PDF (993KB)(2004)       Save

    Rice is one of the primary staple crops. Its yield estimation plays a crucial role in the macro-control of national policies, real-time guidance according to local agricultural conditions, and targeted breeding of elite varieties. Therefore, estimating rice grain yield is of great significance. With the continuous advancement in crop science and interdisciplinary approaches, the methods and models for estimating rice yield have diversified greatly. Concurrently, the development of remote sensing technology, particularly the emergence and popularization of low-altitude Unmanned Aerial Vehicles (UAVs), has led to continuous improvements in intelligent remote sensing methods and their accuracy for rice yield estimation. However, there is a lack of systematic and scientific summarization of intelligent rice yield estimation, specifically utilizing UAV remote sensing. This work provides a comprehensive review of the current mainstream methodologies for estimating rice yield, critically evaluating their advantages and limitations. It then explores the application and future directions of intelligent low-altitude remote sensing technology in rice yield estimation. By analyzing the key characteristic information obtained through remote sensing, the article examines the development of primary yield estimation models. It discusses the challenges and limitations encountered when employing intelligent remote sensing for rice yield estimation. The ultimate goal is to enhance our understanding of rice yield estimation methods and provide a systematic and comprehensive reference for the development of intelligent rice yield estimation techniques.

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