Chinese Journal OF Rice Science >
Breeding and Utilization of the Three-Line Male Sterile Line Fumeng A with Pyramided Rice Blast Resistance Genes Pigm-1 and Pid2
Received date: 2024-12-20
Revised date: 2025-03-25
Online published: 2026-01-21
【Objective】Rice blast is one of the most important diseases in rice production and poses a serious threat to global rice production. The large-scale and long-term planting of varieties with a single resistance gene often leads to the loss of resistance. Molecular marker-assisted selection is one of the most effective approaches for controlling rice blast..【Method】Using Taifeng B as the recipient, and Shuangkang 77009 carrying Pigm-1 and Digu B carrying Pid2 as donors, respectively. The genotypes of each generation were analyzed and identified by means of hybridization, backcrossing, backcrossing and recurred cross, aided by molecular marker-assisted breeding. Subsequently, the blast resistance of the developed sterile line Fumeng A and its hybrids was evaluated using 12 blast isolates from diverse sources under natural field conditions. Finally, through field trials, the fertility, main agronomic traits, combining ability, and heterosis of Fumeng A were systematically analyzed, and the photo-sensitive characteristics of its corresponding restorer line were also examined.【Result】A new three-line male sterile line, Fumeng A, carrying both Pigm-1 and Pid2, was developed. Inoculation tests both in the laboratory and field confirmed its blast resistance. Fertility identification showed that both the sterility rate and sterility degree of Fumeng A reached 100%, with a stigma exsertion rate of 57.39%. Genetic background and agronomic trait analysis indicated that Fumeng A inherited the main agronomic traits of the recurrent parent and exhibited improved yield potential. Heterosis analysis revealed that half of the hybrid combinations derived from Fumeng A yielded higher than the control variety Tianyouhuazhan, demonstrating good potential for production application.【Conclusion】Using molecular marker-assisted selection, a novel three-line male sterile line, Fumeng A (B), with high blast resistance, stable sterility, high stigma exsertion, and excellent agronomic and yield traits was developed. This work achieved targeted improvement of blast resistance in a sterile line and created a new parental material for three-line hybrid rice breeding.
CHENG Zhaoping, HE Niqing, BAI Kangcheng, LIN Shaojun, HUANG Fenghuang, LIU Junhua, CHENG Zuxin, HUANG Chengzhi, YANG Dewei . Breeding and Utilization of the Three-Line Male Sterile Line Fumeng A with Pyramided Rice Blast Resistance Genes Pigm-1 and Pid2[J]. Chinese Journal OF Rice Science, 2026 , 40(1) : 72 -84 . DOI: 10.16819/j.1001-7216.2026.241209
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