Chinese Journal OF Rice Science >
Evaluation of Blast Resistance and Identification of Superior Haplotype of OsDR8 in Rice
Received date: 2024-05-16
Revised date: 2024-07-19
Online published: 2025-05-21
【Objective】Rice (Oryza sativa L.) is a crucial staple crop globally. Its production is severely threatened by rice blast disease. Identifying resistance materials and analyzing resistance genes are essential for understanding the mechanisms behind rice blast resistance. This study aims to systematically generate and identify genetic materials of the rice blast resistance gene OsDR8, conduct haplotype and evolutionary analyses to select superior haplotypes, and investigate their subpopulation differentiation. These efforts will lay a theoretical basis for the application of OsDR8 in rice blast resistance breeding programs.【Method】We developed transgenic lines overexpressing the OsDR8 gene and its knockout mutants. We conducted rice blast resistance assays on the overexpression lines, homozygous knockout lines, and corresponding wild-type materials using Magnaporthe oryzae isolates RO1-1 and RB22. Meanwhile, haplotype and evolutionary analyses of the OsDR8 gene were conducted based on the rice super pan-genome variation map.【Result】Disease symptom observation and disease resistance evaluation indicated that knockout mutants had significantly larger lesion sizes and a higher proportion of diseased leaf area compared to the wild-type. In contrast, the overexpression lines showed reduced lesion sizes and a lower proportion of diseased leaf area. Combined with haplotype and evolutionary analyses, the results indicated that materials of the superior haplotype Hap2 show stronger disease resistance, with 95.52% of the indica rice subpopulation being the Hap2 haplotype.【Conclusion】The OsDR8 gene positively regulates resistance to rice blast, and that the superior haplotype Hap2 may be associated with stronger resistance in indica rice.
Key words： rice; rice blast; superior haplotype; breeding application
ZHANG Bintao, LIU Congcong, GUO Mingliang, YANG Shaohua, WU Shiqiang, GUO Longbiao, ZHU Yiwang . Evaluation of Blast Resistance and Identification of Superior Haplotype of OsDR8 in Rice[J]. Chinese Journal OF Rice Science, 2025 , 39(3) : 343 -351 . DOI: 10.16819/j.1001-7216.2025.240509
| [1] | Skamnioti P, Gurr S J. Against the grain: Safeguarding rice from rice blast disease[J]. Trends in Biotechnology, 2009, 27(3): 141-150. |
| [2] | Jones J D G, Dangl J L. The plant immune system[J]. Nature, 2006, 444(7117): 323-329. |
| [3] | Zhou J M, Zhang Y. Plant immunity: Danger perception and signaling[J]. Cell, 2020, 181(5): 978-989. |
| [4] | Yuan M, Jiang Z, Bi G, Nomura K, Liu M, Wang Y, Cai B, Zhou J M, He S Y, Xin X F. Pattern-recognition receptors are required for NLR-mediated plant immunity[J]. Nature, 2021, 592(7852): 105-109. |
| [5] | Ngou B P M, Ahn H K, Ding P, Jones J D G. Mutual potentiation of plant immunity by cell-surface and intracellular receptors[J]. Nature, 2021, 592(7852): 110-115. |
| [6] | Wang J, Song W, Chai J. Structure, biochemical function, and signaling mechanism of plant NLRs[J]. Molecular Plant, 2023, 16(1): 75-95. |
| [7] | Ding L N, Li Y T, Wu Y Z, Li T, Geng R, Cao J, Zhang W, Tan X L. Plant disease resistance-related signaling pathways: Recent progress and future prospects[J]. International Journal of Molecular Sciences, 2022, 23(24): 16200. |
| [8] | Dong W, Stockwell V O, Goyer A. Enhancement of thiamin content in Arabidopsis thaliana by metabolic engineering[J]. Plant and Cell Physiology, 2015, 56(12): 2285-2296. |
| [9] | Boubakri H, Gargouri M, Mliki A, Brini F, Chong J, Jbara M J P. Vitamins for enhancing plant resistance[J]. Planta, 2016, 244(3): 529-543. |
| [10] | Rapala-Kozik M, Wolak N, Kujda M, Banas A K. The upregulation of thiamine (vitamin B1) biosynthesis in Arabidopsis thaliana seedlings under salt and osmotic stress conditions is mediated by abscisic acid at the early stages of this stress response[J]. BMC Plant Biology, 2012, 12(1): 2. |
| [11] | Ahn I P, Kim S, Lee Y H. Vitamin B1 functions as an activator of plant disease resistance[J]. Plant Physiology, 2005, 138(3): 1505-1515. |
| [12] | Ahn I P, Kim S, Lee Y H, Suh S C. Vitamin B1-induced priming is dependent on hydrogen peroxide and the NPR1 gene in Arabidopsis[J]. Plant Physiology, 2007, 143(2): 838-848. |
| [13] | Bahuguna R N, Joshi R, Shukla A, Pandey M, Kumar J. Thiamine primed defense provides reliable alternative to systemic fungicide carbendazim against sheath blight disease in rice (Oryza sativa L.)[J]. Plant Physiology and Biochemistry, 2012, 57: 159-167. |
| [14] | Wang G, Ding X, Yuan M, Qiu D, Li X, Xu C, Wang S. Dual function of rice OsDR8 gene in disease resistance and thiamine accumulation[J]. Plant Molecular Biology, 2006, 60(3): 437-449. |
| [15] | Xie X, Ma X, Zhu Q, Zeng D, Li G, Liu Y G. CRISPR-GE: A convenient software toolkit for CRISPR-based genome editing[J]. Molecular Plant, 2017, 10(9): 1246-1249. |
| [16] | Shang L, He W, Wang T, Yang Y, Xu Q, Zhao X, Yang L, Zhang H, Li X, Lü Y, Chen W, Cao S, Wang X, Zhang B, Liu X, Yu X, He H, Wei H, Leng Y, Shi C, Guo M, Zhang Z, Zhang B, Yuan Q, Qian H, Cao X, Cui Y, Zhang Q, Dai X, Liu C, Guo L, Zhou Y, Zheng X, Ruan J, Cheng Z, Pan W, Qian Q. A complete assembly of the rice Nipponbare reference genome[J]. Molecular Plant, 2023, 16(8): 1232-1236. |
| [17] | Shang L, Li X, He H, Yuan Q, Song Y, Wei Z, Lin H, Hu M, Zhao F, Zhang C, Li Y, Gao H, Wang T, Liu X, Zhang H, Zhang Y, Cao S, Yu X, Zhang B, Zhang Y, Tan Y, Qin M, Ai C, Yang Y, Zhang B, Hu Z, Wang H, Lü Y, Wang Y, Ma J, Wang Q, Lu H, Wu Z, Liu S, Sun Z, Zhang H, Guo L, Li Z, Zhou Y, Li J, Zhu Z, Xiong G, Ruan J, Qian Q. A super pan-genomic landscape of rice[J]. Cell Research, 2022, 32(10): 878-896. |
| [18] | Cingolani P, Platts A, Wang L L, Coon M, Nguyen T, Wang L, Land S J, Lu X, Ruden D M. A program for annotating and predicting the effects of single nucleotide polymorphisms, SnpEff: SNPs in the genome of Drosophila Melanogaster strain w1118; iso-2; iso-3[J]. Fly, 2012, 6(2): 80-92. |
| [19] | Hothorn T, Bretz F, Westfall P. Simultaneous inference in general parametric models[J]. Biometrical Journal, 2008, 50(3): 346-363. |
| [20] | Paradis E. PEGAS: An R package for population genetics with an integrated-modular approach[J]. Bioinformatics, 2010, 26(3): 419-420. |
| [21] | Leigh J W, Bryant D. PopART: Full-feature software for haplotype network construction[J]. Methods in Ecology and Evolution, 2015, 6(9): 1110-1116. |
| [22] | Katoh K, Standley D M. MAFFT multiple sequence alignment software version 7: Improvements in performance and usability[J]. Molecular Biology and Evolution, 2013, 30(4): 772-780. |
| [23] | Tamura K, Stecher G, Kumar S. MEGA11: Molecular evolutionary genetics analysis version 11[J]. Molecular Biology and Evolution, 2021, 38(7): 3022-3027. |
| [24] | Ogata Y, Kimura N, Sano R. Gcorn plant: A database for retrieving functional and evolutionary traits of plant genes[J]. Plant Physiology, 2019, 180(2): 732-742. |
| [25] | Chow C N, Lee T Y, Hung Y C, Li G Z, Tseng K C, Liu Y H, Kuo P L, Zheng H Q, Chang W C. PlantPAN3.0: A new and updated resource for reconstructing transcriptional regulatory networks from ChIP-seq experiments in plants[J]. Nucleic Acids Research, 2019, 47(D1): D1115-D1163. |
| [26] | Liu M H, Kang H, Xu Y, Peng Y, Wang D, Gao L, Wang X, Ning Y, Wu J, Liu W, Li C, Liu B, Wang G L. Genome-wide association study identifies an NLR gene that confers partial resistance to Magnaporthe oryzae in rice[J]. Plant Biotechnology Journal, 2020, 18(6): 1376-1383. |
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