Chinese Journal OF Rice Science >
Gene Cloning and Broad-spectrum Disease Resistance Analysis of Rice Lesion Mimic Mutant lm52
Received date: 2024-03-04
Revised date: 2024-05-06
Online published: 2025-05-21
【Objective】Lesion mimic mutants are ideal materials to study the mechanisms of plant cell death and disease resistance, offering new strategies for plant disease resistance breeding.【Method】The lm52 mutant gene was cloned by the map-based cloning method, and the function of the candidate gene was verified by CRISPR/Cas9 knockout technology. Meanwhile, the resistance of lm52 to multiple rice pathogens and the possible resistance mechanisms were analyzed.【Result】The mutant lm52 gradually developed progressive brown spot lesions from the 4th leaf stage, with whole leaf necrosis at maturity stage. A single base mutation (G1440A) on the ATPase encoding gene LOC_Os06g03940 caused the lesion mimic phenotype of lm52. lm52 is characterized by significantly enhanced resistance to blast, sheath blight and bacterial blight, which was found to be related to the overaccumulation of ROS and the activation of defense-related genes.【Conclusion】The mutation of the ATPase gene LM52 enhances rice resistance against diverse pathogens, and provides potential target genes for molecular breeding of rice disease resistance.
Key words： rice; lesion mimic mutant; map-based cloning; disease-resistance
HUANG Tao, WEI Zhaogen, CHENG Qi, CHENG Ze, LIU Xin, WANG Guangda, HU Keming, XIE Wenya, CHEN Zongxiang, FENG Zhiming, ZUO Shimin . Gene Cloning and Broad-spectrum Disease Resistance Analysis of Rice Lesion Mimic Mutant lm52[J]. Chinese Journal OF Rice Science, 2025 , 39(3) : 322 -330 . DOI: 10.16819/j.1001-7216.2025.240304
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