Chinese Journal OF Rice Science >
Characterization and Transcriptome Analysis of a Mutant with Short Panicle and Small Grain from Zhejing 99
Received date: 2023-11-14
Revised date: 2024-03-04
Online published: 2025-01-14
【Objective】This study aims to explore the formation mechanism of the superior panicle type in Zhejing 99 and lays a scientific foundation for understanding the developmental mechanisms of rice panicle types and their breeding applications.【Method】We used ethyl methanesulfonate (EMS) to induce mutations in Zhejing 99, resulting in a short panicle and small grain mutant. Molecular markers were employed to obtain linkage intervals, and candidate genes were identified through RNA sequencing (RNA-Seq) and DNA sequencing. The subcellular localization of the mutant gene was observed using a laser confocal microscopy. Additionally, transcriptome sequencing was conducted to analyze gene expression and the expression characteristics of related genes.【Result】Phenotypic identification revealed differences in plant type, panicle type, and grain type of the Ossp3 mutant compared to Zhejing 99. The mutant phenotype was caused by a base insertion in the candidate gene Os02g0450000. Subcellular localization showed that the gene product is located in the nucleus. In the young panicles of the Ossp3 mutant, 329 differentially expressed genes (DEGs) were identified, primarily distributed on chromosomes 1, 3, and 4. KEGG enrichment analysis highlighted significant biological processes, including plant hormone signaling pathways and MAPK signaling pathways in plants. Analysis of related gene expression suggests that the evolutionarily conserved plant-specific gene OsSP3 influences auxin and cytokinin content and their corresponding signaling pathways through the MAPK signaling pathway or by interacting with bZIP transcription factors, thereby regulating rice panicle development.【Conclusion】The gene mutation in the superior panicle type variety Zhejing 99, specifically in Os02g0450000, results in the phenotype of short panicles and small grains. Its biological function is mediated through nuclear localization. RNA-Seq analysis indicates that the mutated gene may synergistically regulate the growth and development of rice panicles by affecting auxin and cytokinin signaling pathways and may also directly or indirectly influence grain type through interaction with OsbZIP47.
LIANG Chuyan, ZENG Wei, WANG Jiebing, YE Jing, WU Mingming, ZHAI Rongrong, ZHANG Xiaoming, ZHANG Hengmu, YE Shenghai . Characterization and Transcriptome Analysis of a Mutant with Short Panicle and Small Grain from Zhejing 99[J]. Chinese Journal OF Rice Science, 2025 , 39(1) : 67 -81 . DOI: 10.16819/j.1001-7216.2024.231111
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