Chinese Journal OF Rice Science >
Development and Application of a Functional Marker for Heading Date Gene Hd6 in Rice
Received date: 2023-10-24
Revised date: 2023-11-30
Online published: 2025-01-14
【Objective】This study aims to explore the distribution of Hd6 alleles in japonica rice and indica rice from Jiangsu Province, and to enhance the application value and selection efficiency of Hd6 alleles in the genetic improvement of rice heading date. 【Method】We designed and screened various types of gene functional markers based on the single nucleotide polymorphisms in the functional regions of the Hd6 alleles in Kasalath and hd6 in Nipponbare. Through PCR amplification and sequencing, we selected the gene markers K-Hd6-21F/Hd6-1R and N-Hd6-22F/Hd6-1R to genotype japonica rice varieties from different ecological types in Jiangsu Province and indica rice varieties from various sources.【Result】We found that K-Hd6-21F/Hd6-1R and N-Hd6-22F/Hd6-1R could effectively distinguish different genotypes at the Hd6 locus. The results indicated that 42% of the 48 medium-maturing japonica rice varieties carried the Hd6 allele, 50% of the 30 late-maturing japonica rice varieties carried Hd6, and 77.5% of the 48 early-maturing japonica rice varieties carried Hd6. The two alleles of Hd6 were distributed among varieties from three different ecological regions in Jiangsu Province, and the genotype frequency of functional Hd6 alleles increased with the heading date. The Hd6 gene is highly conserved in indica rice and was tested in all 62 indica varieties.【Conclusion】This study lays a foundation for the utilization of the Hd6 gene and molecular marker-assisted selection in rice breeding, providing a new basis and effective approach for the breeding of early-maturing varieties in indica rice.
Key words： rice; heading date; Hd6; functional marker; allele-specific PCR
CHEN Zhihui, TAO Yajun, FAN Fangjun, XU Yang, WANG Fangquan, LI Wenqi, GULINAER·Bahetibieke , JIANG Yanjie, ZHU Jianping, LI Xia, YANG Jie . Development and Application of a Functional Marker for Heading Date Gene Hd6 in Rice[J]. Chinese Journal OF Rice Science, 2025 , 39(1) : 47 -54 . DOI: 10.16819/j.1001-7216.2025.231009
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