Chinese Journal OF Rice Science >
Effects of 2,4-Epibrassinolide on Root Growth and Physiological Characteristics of Rice Seedlings Raised in Biochar Substrate
Received date: 2024-05-06
Revised date: 2024-05-31
Online published: 2024-11-15
【Objective】The improved knotting ability of roots enhances the efficiency and quality of mechanical transplanting. This study investigates the effects and mechanisms of 2,4-epibrassinolide (EBR) on rice root growth in rice straw biochar substrate.【Method】The effects of different concentrations of EBR on the root growth and physiological characteristics of rice seedlings grown in biochar substrate prepared at 300°C were studied using Yongyou 538 as the experimental material.【Result】When EBR was added to the biochar substrate, the root cohesion of rice seedlings increased by 4.13% to 22.46%, and root activity increased by 0.93 to 1.65 times, which improved the stress resistance of rice and promoted root growth. The addition of EBR to the substrate significantly increased the activities of antioxidant protective enzymes such as superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in rice seedlings. It also decreased the contents of malondialdehyde (MDA) and hydrogen peroxide (H2O2), while increasing the contents of soluble sugar and soluble protein in the seedlings. The expression levels of OsCu/Zn-SOD1, OsCu/Zn-SOD2, OsCAT1, and OsCAT2 genes in the roots and leaves of rice treated with EBR were significantly higher than those in the control group.【Conclusion】When the concentration of EBR was between 1.0 and 1.5 mg/kg in the biochar substrate, root growth of rice seedlings was promoted, enzyme activities of the antioxidant system were enhanced, and the expression of related genes in the seedlings was improved. Additionally, the contents of MDA and H2O2 in the plants were reduced, leading to improved root growth and knotting ability of rice seedlings, thereby promoting the formation of healthy seedlings.
Key words： biochar substrate; rice; roots; 2, 4-epibrassinolide; antioxidant enzymes; gene expression
WU Xiang, ZHANG Yikai, ZHANG Peng, MA Xinling, CHEN Yulin, CHEN Huizhe, ZHANG Yuping, XIANG Jing, WANG Yaliang, WANG Zhigang, LI Liangtao . Effects of 2,4-Epibrassinolide on Root Growth and Physiological Characteristics of Rice Seedlings Raised in Biochar Substrate[J]. Chinese Journal OF Rice Science, 2024 , 38(6) : 685 -694 . DOI: 10.16819/j.1001-7216.2024.240503
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