Chinese Journal OF Rice Science >
Development and Application of Specific Molecular Markers for Eight Rice Blast Resistance Genes in Rice
Received date: 2023-08-31
Revised date: 2023-11-17
Online published: 2024-09-10
【Objective】To elucidate the composition of blast resistance genes in rice parents and facilitate their effective utilization in rice resistance breeding, it’s crucial to develop molecular markers that transcend genetic background limitations. These molecular markers can accurately discern resistance genes in parents, serving as essential tools for marker-assisted breeding.【Method】By comparing the coding sequence of each Resistance gene with allelic genes in 155 rice resources, nucleotide polymorphism sites within each resistance gene were identified. Primer pairs for molecular markers were then developed targeting the most specific polymorphism sites.【Results】Effective molecular markers were successfully developed for eight resistance genes: Pit, Pish, Pib, Pid3, Pi5, Pia, Pi54, and Pita2/Ptr. These markers were validated through examination using multiple positive and negative controls or gene sequencing. Subsequently, these markers were utilized to identify the resistance gene composition in 109 breeding parent lines commonly used in the Sichuan Basin. Results revealed that the Pia gene was absent in these parent lines, while Pit, Pish, Pi54, Pid3, Ptr/Pita2, Pi5, and Pib genes were present in 3.67%, 13.76%, 14.68%, 18.35%, 24.77%, 26.61%, and 38.53% of lines, respectively. Furthermore, 21.10% of the rice parent lines lacked these resistance genes, 35.78% possessed one resistance gene, and 43.12% harbored two to four resistance genes.
Key words： rice; rice blast disease; resistance genes; molecular markers
YANG Hao, HUANG Yanyan, WANG Jian, YI Chunlin, SHI Jun, TAN Chutian, REN Wenrui, WANG Wenming . Development and Application of Specific Molecular Markers for Eight Rice Blast Resistance Genes in Rice[J]. Chinese Journal OF Rice Science, 2024 , 38(5) : 525 -534 . DOI: 10.16819/j.1001-7216.2024.230811
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