Chinese Journal OF Rice Science >
Relationship Between Embryo Survival and Glucose Supply of Rice Seed and Embryo Under Flooding Stress
Received date: 2023-09-04
Revised date: 2024-01-05
Online published: 2024-03-14
【Objective】To investigate varietal differences in germ glucose supply under flooded conditions and its relationship with rice germ survival.【Method】Germinated seeds of four rice varieties with different flooding tolerance were used as material and subjected to flooding and wet (control) conditions. Dynamic changes of non-structural carbohydrate contents in seed and germ tissue, seed α-amylase activities, and germ antioxidant enzyme activities over time were analyzed, along with their correlation with seed survival rates.【Result】Under flooding stress, the glucose content and the activities of SOD and POD in the germs of the flooding-sensitive rice materials, Zhongjiazao 8(S1) and Zhongjiazao 17(S2), decreased significantly after 72 hours of flooding, leading to stagnation of germ growth and death. However, the embryo glucose content and the activities of SOD and POD remained stable in the germs of the flooding-tolerant variety Nipponbare(T1) and Yunliangyoujiu 48(T2) after 96 hours of flooding treatment, allowing the germs to continue growing and elongating. Analysis of seed α-amylase activities showed that while short-term flooding (0－48h) enhanced seed α-amylase activities in all four materials, only flooding-tolerant varieties T1 and T2 maintained high α-amylase activity under prolonged flooding (72－96h), leading to a decline in starch content within their seeds and an increase in glucose content. In contrast, the α-amylase activities of flood-sensitive varieties S1 and S2 declined after 48 and 72 hours of flooding treatment, respectively, with relatively stable starch content in the seeds but substantial declines in glucose content of the seeds and the germ. Correlation analysis revealed that germ survival was highly positively correlated with germ and seed glucose content, and POD activity of germ, while highly negatively correlated with seed starch content.【Conclusion】Under flooding stress, the germ tissue consumes a large amount of glucose to maintain its physiological metabolic balance, and the ability of seeds to supply glucose to the embryo is directly related to its survival. Changes in seed α-amylase activity play a pivotal role in the glucose supply to the germ, which is crucial for the survival and emergence of the seed under flooding stress.
Key words： flooding; rice seeds; embryo survival rate; glucose; α-amylase; antioxidant enzyme
ZHENG Guangjie, YE Chang, ZHU Junlin, TAO Yi, XIAO Deshun, XU Yanan, CHU Guang, XU Chunmei, WANG Danying . Relationship Between Embryo Survival and Glucose Supply of Rice Seed and Embryo Under Flooding Stress[J]. Chinese Journal OF Rice Science, 2024 , 38(2) : 172 -184 . DOI: 10.16819/j.1001-7216.2024.230902
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