Chinese Journal OF Rice Science >
Characterization and Gene Mapping of a Glume Lesion Mimic Mutant glmm1 in Rice
#These authors contributed equally to this work
Received date: 2022-12-05
Revised date: 2023-01-31
Online published: 2023-09-13
【Objective】 The aim of this study is to identify and map the glume lesion mimics mutant gene in rice, and to lay a foundation for gene cloning and molecular mechanism research. 【Method】 Agronomic traits analysis, scanning electron microscopy observation, DAB staining, and total silicon content determination were performed on the wild-type material LR005 and the glume lesion mimics mutant glmm1 obtained through EMS mutagenesis. The F2 population derived from glmm1 and L422 was used for genetic analysis, and gene mapping by map-based cloning and BSA-seq method. 【Result】 The mutant gradually showed brown spots on the glumes and leaves 10 days after heading, and the glumes were completely brown at mature stage. Compared with the wild type, the mutant showed highly significant reductions in plant height, panicle length, total number of grains per panicle, seed setting rate and 1000-grain weight. DAB staining showed that the glmm1 glumes and leaves had increased ROS(reactive oxygen species) content; Scanning electron microscopy observation showed that the siliceous cells on the surface of the mutant glumes and leaves wrinkled. The genetic analysis showed that the glume lesion mimics phenotype of glmm1 was controlled by a pair of recessive genes. Using the F2 segregating population of glmm1 and L422, GLMM1 was localized to a 68-kb interval containing 10 genes on chromosome 2 by map-based cloning and BSA-seq. Sequence analysis revealed only one SNP in this interval, which located in the fifth exon of the gene Lsi1 (LOC_Os02g51110), resulting in a substitution of Ile with Thr. The reduced accumulation of glmm1 silicon was found by measuring total silicon content of the glumes and leaves, suggesting that glmm1 may be a mutant allele of Lsi1. 【Conclusion】 glmm1 is a new mutant allele of Lsi1, which causes a reduction in silicon content and accumulation of reactive oxygen species in plants, resulting in brown spots on glumes and leaves.
Key words： rice; mutant; lesion mimic; silicon transporters; gene mapping
XU Huan, ZHOU Tao, SUN Yue, WANG Mumei, YANG Yachun, MA Hui, LI Hao, XU Dawei, ZHOU Hai, YANG Jianbo, NI Jinlong . Characterization and Gene Mapping of a Glume Lesion Mimic Mutant glmm1 in Rice[J]. Chinese Journal OF Rice Science, 2023 , 37(5) : 497 -506 . DOI: 10.16819/j.1001-7216.2023.221201
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