Chinese Journal OF Rice Science >
Development of Aromatic Salt-tolerant Rice Based on CRISPR/Cas9 Technology
Received date: 2022-09-28
Revised date: 2022-12-19
Online published: 2023-09-13
【Objective】 It is of importance to promote salt-tolerant and fragrant rice breeding. We obtained transgene free homozygous rice materials with salt-tolerance and fragrance by CRISPR/Cas9-mediated editing of Badh2 and OsRR22 in japonica variety Lianjing 11. 【Method】 Target sites were designed according to knockout efficiency of editing sites in Badh2 and OsRR22 gene sequence. The pH-Ubi-Badh2-OsRR22 knockout vector was constructed and transferred into the recipient variety Lianjing 11 by the Agrobacterium-mediated technology. PCR detection for hygromycin, Cas9 marker and target gene sequencing were performed in the transgenic progenies to obtain badh2-osrr22 homozygous lines without exogenous gene insertion. The seed traits and salt tolerance at seedling stage were analyzed. 【Result】 Compared with the background material Lianjing 11, the 2-AP content of T2 significantly increased. After treated with 128 mmol/L NaCl solution for 14 days, the seedling height, the fresh and the dry weight of T2 line 21-30 increased by 15.2%, 45.2% and 13.2%, respectively. 【Conclusion】 The successful editing of Badh2 and OsRR22 by CRISPR/Cas9 technology developed aromatic salt-tolerant homozygous lines without exogenous gene insertion, speeding up the breeding process of pyramiding multiple traits in rice.
Key words： rice; CRISPR/Cas9; salt-tolerant; fragrance; Badh2; OsRR22
LI Jingfang, WEN Shuyue, ZHAO Lijun, CHEN Tingmu, ZHOU Zhenling, SUN Zhiguang, LIU Yan, CHEN Haiyuan, ZHANG Yunhui, CHI Ming, XING Yungao, XU Bo, XU Dayong, WANG Baoxiang . Development of Aromatic Salt-tolerant Rice Based on CRISPR/Cas9 Technology[J]. Chinese Journal OF Rice Science, 2023 , 37(5) : 478 -485 . DOI: 10.16819/j.1001-7216.2023.220907
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