Chinese Journal OF Rice Science >
Identification and Gene Mapping of Outcurved Leaf Mutant ocl1 in Rice
Received date: 2022-10-20
Revised date: 2022-11-22
Online published: 2023-07-17
【Objective】 Moderate leaf rolling can enhance photosynthetic efficiency and improve rice yield. Identification of rice leaf rolling mutants will help elucidate the molecular mechanism behind rice leaf formation. 【Methods】 60Co-γ ray irradiation was used to mutagenize indica rice variety Yuzhenxiang and an outcurved leaf mutant was obtained, tentatively named ocl1 (outcurved leaf 1). The phenotypic and agronomic traits of the mutant were investigated. The F2 population of a cross between ocl1 and 02428 was used for mapping of the OCL1 gene. Additionally, the expression of leaf-rolling related genes was analyzed by quantitative PCR. 【Result】 Compared with the wild-type, the leaves of the mutant was characterized by outcurved and drooping leaves from tillering to mature stage. The yield components including the seed setting rate, 1000-grain weight and yield per plant were obviously decreased in ocl1. Cross-section observation of leaves revealed that the bulliform cells between adjacent vascular bundles of ocl1 were larger than those of the wild-type. Genetic analysis implied that these phenotypes of the mutant were controlled by a pair of recessive nuclear gene. The OCL1 gene was fine-mapped to a 127 kb interval between markers RM19575 and ID02612 on the chromosome 6. Sequencing analysis indicated that one of the genes (LOC_Os06g10600) has a single-base mutation at the intron-exon junction, which leads to abnormal splicing and changes in amino acid sequence. The gene encodes a homologous domain-leucine zipper protein, which is an allele to the leaf rolling related gene ROC8 (URL1). Correspondingly, the expression of ROC5 and LAC17 genes related to bulliform cell development was down-regulated in ocl1, while that of XTH11 was up-regulated in ocl.【Conclusion】 The mutation of OCL1 gene results in outcurved leaf by affecting the development of bulliform cells, which in turn leads to a yield reduction.
Key words： rice; outcurved leaf; alternative splicing; bulliform cell
REN Zhiqi, XUE Kexin, DONG Zheng, LI Xiaoxiang, LI Yongzhao, GUO Yujing, LIU Wenqiang, GUO Liang, SHENG Xinnian, LIU Zhixi, PAN Xiaowu . Identification and Gene Mapping of Outcurved Leaf Mutant ocl1 in Rice[J]. Chinese Journal OF Rice Science, 2023 , 37(4) : 337 -346 . DOI: 10.16819/j.1001-7216.2023.221004
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