Chinese Journal OF Rice Science >
Effect of Different Fertilizer Application Rates on Rice Yield and Quality Formation and Its Relationship with Energy Metabolism at High Temperature
Received date: 2022-08-25
Revised date: 2022-12-05
Online published: 2023-05-16
【Objective】 In recent years, frequent occurrence of extreme high temperature weather severely inhibited the formation of rice yield and quality. Optimizing plant nutritional status and improving energy metabolism can alleviate thermal damage. However, the effects of fertilizer application rates on grain energy metabolism and its relationship with yield, quality and thermal resistance at high temperature have not been reported, and elucidating the mechanism can lay an important theoretical basis for the research and development of rice heat-resistant and risk-resistant cultivation technology.【Method】 In this study, a single cropping indica hybrid rice variety Zhongzheyou 8, which was widely planted in Zhejiang Province in recent years, was pot-cultivated in a climate chamber. Three fertilizer treatments were set: no fertilization (0-NPK), 1/2 normal fertilization (1/2-NPK) and normal fertilization (1-NPK). On the day of flowering, they were moved to the artificial climate chamber for high temperature treatment for 15d (for high temperature treatment, 36℃ in daytime and 28℃ at night; for the normal temperature treatment, 28℃ in daytime and 25℃ at night).【Result】The yield, seed-setting rate and 1000-grain weight of Zhongzheyou 8 followed a trend of increasing with the increaing fertilizer application at normal temperature or high temperature, with a shrinking decreasing amplitude at high temperature. However, head rice rate decreased with the increase of fertilizer application, and chalkiness degree showed an upward trend. The contents of soluble sugar, MDA, H2O2, ATPase and poly(ADP-ribose) polymerase (PARP) in the grains after high temperature treatment were higher than those in the normal temperature control, but contents of starch, non-structural carbohydrate, antioxidant enzyme and ATP were lower than those in the normal temperature control. Both at normal temperature and high temperature, the activities of non-structural carbohydrates, antioxidant enzymes, ATP and ATPase increased with rising fertilizer application level, while contents of MDA, H2O2 and PARP decreased. At normal temperature, relative expression levels of energy receptor genes SnRK1A and SnRK1B decreased with the increase of fertilizer application, while TOR showed an increasing trend. Compared with normal temperature treatment, after high temperature treatment, the expression of SnRK1A was upregulated, while TOR was downregulated with the increase of fertilizer amount.【Conclusion】Increased fertilizer application could effectively alleviate the negative effect of high temperature on seed setting rate and 1000-grain weight of rice during flowering, but appearance quality and processing quality tended to deteriorate. At normal temperature, the quality deterioration caused by increased fertilizer application may be related to the acceleration of grain filling, while the quality deterioration caused by high temperature is mainly due to the lack of energy, which is difficult to meet the demand of yield and quality formation.
WANG Wenting, MA Jiaying, LI Guangyan, FU Weimeng, LI Hubo, LIN Jie, CHEN Tingting, FENG Baohua, TAO Longxing, FU Guanfu, QIN Yebo . Effect of Different Fertilizer Application Rates on Rice Yield and Quality Formation and Its Relationship with Energy Metabolism at High Temperature[J]. Chinese Journal OF Rice Science, 2023 , 37(3) : 253 -264 . DOI: 10.16819/j.1001-7216.2023.220808
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