Chinese Journal OF Rice Science >
Development of New Low Glutelin Content japonica Rice Lines with Good Eating Quality and Fragrance by Molecular Marker-Assisted Selection
Received date: 2022-03-01
Revised date: 2022-06-06
Online published: 2023-01-10
【Objective】 Developing new low glutelin content varieties is an important direction of functional rice breeding. It is necessary to strengthen the synchronized improvement of rice functionality and eating quality in breeding to meet the needs of nephropathic patients for rice quality and physical health. 【Method】 The good eating quality japonica rice variety Nanjing 46 with the low amylose content gene Wxmp and fragrance gene fgr was used as the parent to cross and backcross with the variety LGC-1 from Japan containing the Lgc1 gene. The molecular markers co-isolated with target genes were utilized for genotypic detection in combination with field selection and five new lines were obtained in BC2F6 generation. Taking parents Nanjing 46 and LGC-1 as control, the agronomic, yield and quality traits of these lines were analyzed. 【Result】 Compared with LGC-1, these lines has similar glutelin content and absorbable protein content, improved eating quality, excellent comprehensive characters and high yield potential. They are suitable to grow in different regions of Jiangsu Province. 【Conclusion】 As a rapid, accurate and effective method, the molecular marker-assisted selection, in combination with conventional breeding technology, can significantly improve the breeding efficiency of the low gluten rice varieties with good quality and high yield.
CHEN Tao, ZHAO Qingyong, ZHU Zhen, ZHAO Ling, YAO Shu, ZHOU Lihui, ZHAO Chunfang, ZHANG Yadong, WANG Cailin . Development of New Low Glutelin Content japonica Rice Lines with Good Eating Quality and Fragrance by Molecular Marker-Assisted Selection[J]. Chinese Journal OF Rice Science, 2023 , 37(1) : 55 -65 . DOI: 10.16819/j.1001-7216.2023.220302
| [1] | 中国水稻研究所, 国家水稻产业技术研发中心. 2021年中国水稻产业发展报告[M]. 北京: 中国农业科学技术出版社, 2021: 148-154. |
| [1] | China National Rice Research Institute, National Rice Industry Technology Research and Development Center. Report on the development of China's rice industry in 2021[M]. Beijing: China Agricultural Science and Technology Press, 2021: 148-154 (in Chinese) |
| [2] | 陈静, 唐振闯, 程广燕. 我国稻谷口粮消费特征及其趋势预测[J]. 中国农业资源与区划, 2020, 41(4): 108-116. |
| [2] | Chen J, Tang Z C, Cheng G Y. Edible rice consumption characteristics and trend prediction in China[J]. Chinese Journal of Agricultural Resources and Regional Planning, 2020, 41(4): 108-116. (in Chinese with English abstract) |
| [3] | 刘巧泉, 周丽慧, 王红梅, 顾铭洪. 水稻种子贮藏蛋白合成的分子生物学研究进展[J]. 分子植物育种, 2008, 6(1): 1-15. |
| [3] | Liu Q Q, Zhou L H, Wang H M, Gu M H. Advanced on biosynthesis of rice seed storage proteins in molecular biology[J]. Molecular Plant Breeding, 2008, 6(1): 1-5. (in Chinese with English abstract) |
| [4] | 江绍玫, 徐朗莱, 万建民. 水稻谷蛋白研究进展[J]. 江西农业大学学报, 2002, 24(1): 14-19. |
| [4] | Jiang S M, Xu L L, Wan J M. Advance on the glutelin research on rice[J]. Acta Agriculturae Universitatis Jiangxiensis, 2002, 24(1): 14-19. (in Chinese with English abstract) |
| [5] | GBD Chronic Kidney Disease Collaboration. Global, regional, and national burden of chronic kidney disease, 1990-2017: A systematic analysis for the global burden of disease study 2017[J]. The Lancet, 2020, 395(10225): 709-733. |
| [6] | Modification of Diet in Renal Disease Study Group. Effects of dietary protein restriction on the progression of moderate renal disease in the modification of diet in renal disease study[J]. Journal of the American Society of Nephrology, 1996, 7(12): 2616-2626. |
| [7] | Iida S, Amano E, Nishio T. A rice (Oryza sativa L.) mutant having a low content of glutelin and a high content of prolamine[J]. Theoretical and Applied Genetics, 1993, 87(3): 374-378. |
| [8] | Iida S, Kusaba M, Nishio T. Mutants lacking glutelin subunits in rice: Mapping and combination of mutated glutelin genes[J]. Theoretical and Applied Genetics, 1997, 94(2): 177-183. |
| [9] | Miyahara K, Kusaba M, Sassa E, Iida S, Takano T, Nishio T. Analysis of glutelin gene in rice low glutelin line ‘LGC-1’[J]. Breeding Science, 1996, 46(S1): 42. |
| [10] | Kusaba M, Miyahara K, Iida S, Fukuoka H, Takano T, Sassa H, Nishio N. Low glutelin content1: A dominant mutation that suppresses the glutelin multigene family via RNA silencing in rice[J]. Plant Cell, 2003, 15(6): 1455-1467. |
| [11] | Fukuoka R, Hirabayashi H, Nishida M, Fukaura S, Yamashita H, Nishio T, Iida S, Yagi T. Breeding of new rice line “Saikai 231” with low glutelin content[J]. Breeding Science, 1996, 46(Sl): 223. |
| [12] | Iida S, Sunohara Y, Maeda H, Matsushita K, Nemoto H, Ishii T, Yoshida T, Nakagawa N, Sakai M, Nishio T. A new rice cultivar with good eating quality (low amylose) and low glutelin protein, ‘LGC soft’[J]. Bulletin of the National Agricultural Research Center for Western Region (Japan), 2004, 3: 57-74. (in Japanese with English abstract) |
| [13] | Nishimura M, Kusaba M, Miyahara K, Nishio T, Iida S, Imbe T, Sato H. New rice varieties with low levels of easy-to-digest protein, ‘LGC-Katsu’ and ‘LGC-Jun’[J]. Breeding Science, 2005, 55(1): 103-105. |
| [14] | 万建民, 翟虎渠, 刘世家, 江玲, 杨世湖, 陈亮明, 王春明. 功能性专用水稻品种W3660的选育[J]. 作物杂志, 2004, 20(5): 58. |
| [14] | Wan J M, Zhai H Q, Liu S J, Jiang L, Yang S H, Chen L M, Wang C M. Breeding of rice variety W3660 with special function[J]. Crops, 2004, 20(5): 58. (in Chinese) |
| [15] | 陈达刚, 周新桥, 刘传光, 李丽君, 李巨昌, 陈友订. 应用分子标记辅助选择培育籼型低谷蛋白水稻品系[J]. 分子植物育种, 2016, 14(7): 1753-1758. |
| [15] | Chen D G, Zhou X Q, Liu C G, Li L J, Li J C, Chen Y D. Breeding of indica rice lines with low glutelin content by molecular marker-assisted selection[J]. Molecular Plant Breeding, 2016, 14(7): 1753-1758. (in Chinese with English abstract) |
| [16] | 张云辉, 张所兵, 周金云亮, 林静, 汪迎节, 方先文. 水稻低谷蛋白创新种质的选育和鉴定[J]. 植物遗传资源学报, 2015, 16(1): 158-162. |
| [16] | Zhang Y H, Zhang S B, Zhou J Y L, Lin J, Wang Y J, Fang X W. Enhancement and identification of new rice germplasms with low glutelin content[J]. Journal of Plant Genetic Resources, 2015, 16(1): 158-162. (in Chinese with English abstract) |
| [17] | 蔡金洋, 杨尧城, 徐伟东, 李白, 李军. 利用分子标记辅助选育低谷蛋白水稻株系[J]. 浙江农业学报, 2015, 27(9): 1505-1509. |
| [17] | Cai J Y, Yang Y C, Xu W D, Li B, Li J. Breeding of rice lines with low glutelin content by molecular marker- assisted selection[J]. Acta Agriculturae Zhejiangensis, 2015, 27(9): 1505-1509. (in Chinese with English abstract) |
| [18] | 郭涛, 王海风, 薛芳, 房文文, 林香青, 张士永. 利用分子标记辅助选择低谷蛋白水稻新品种. 山东农业科学, 2018, 50(8): 29-34. |
| [18] | Guo T, Wang H F, Xue F, Fang W W, Lin X Q, Zhang S Y. Breeding of rice varieties with low glutelin content by molecular marker-assisted selection. Shandong Agricultural Sciences, 2018, 50(8): 29-34. (in Chinese with English abstract) |
| [19] | 王才林, 张亚东, 赵春芳, 魏晓东, 姚姝, 周丽慧, 朱镇, 陈涛, 赵庆勇, 赵凌, 路凯, 梁文化. 江苏省优良食味粳稻的遗传与育种研究[J]. 遗传, 2021, 43(5): 442-458. |
| [19] | Wang C L, Zhang Y D, Zhao C F, Wei X D, Yao S, Zhou L H, Zhu Z, Chen T, Zhao Q Y, Zhao L, Lu K, Liang W H. Inheritance and breeding of japonica rice with good eating quality in Jiangsu province[J]. Hereditas (Beijing), 2021, 43(5): 442-458. (in Chinese with English abstract) |
| [20] | Murray M G, Thompson W F. Rapid isolation of high molecular-weight plant DNA[J]. Nucleic Acids Research, 1980, 19(8): 4321-4325. |
| [21] | Chen T, Tian M X, Zhang Y D, Zhu Z, Zhao L, Zhao Q Y, Lin J, Zhou L H, Wang C L. Development of simple functional markers for low glutelin content gene 1 (Lgc1) in rice (Oryza sativa)[J]. Rice Science, 2010, 17(3): 173-178. |
| [22] | 陈涛, 骆名瑞, 张亚东, 朱镇, 赵凌, 赵庆勇, 周丽慧, 姚姝, 于新, 王才林. 利用四引物扩增受阻突变体系PCR技术检测水稻低直链淀粉含量基因Wx-mq[J]. 中国水稻科学, 2013, 27(5): 529-534. |
| [22] | Chen T, Luo M R, Zhang Y D, Zhu Z, Zhao L, Zhao Q Y, Zhou L H, Yao S, Yu X, Wang C L. Detection of Wx-mq gene for low-amylose content by tetra-primer amplification refractory mutation system PCR in rice[J]. Chinese Journal Rice Science, 2013, 27(5): 529-534. (in Chinese with English abstract) |
| [23] | 王军, 杨杰, 陈志德, 仲维功. 水稻香米基因标记的开发与应用[J]. 分子植物育种, 2008, 6(6): 1209-1212. |
| [23] | Wang J, Yang J, Chen Z D, Zhong W G. Development and application of fragrance gene markers in rice[J]. Molecular Plant Breeding, 2008, 6(6): 1209-1212. (In Chinese with English abstract) |
| [24] | 江绍玫, 朱速松, 刘世家, 江玲, 徐朗莱, 万建民. 水稻谷蛋白突变体的筛选及遗传分析[J]. 遗传学报, 2003, 30(7): 641-645. |
| [24] | Jiang S M, Zhu S S, Liu S J, Jiang L, Xu L L, Wan J M. Screening and genetic analysis of rice glutelin mutant[J]. Acta Genetica Sinica, 2003, 30(7): 641-645. (in Chinese with English abstract) |
| [25] | Liu Z H, Cheng F M, Cheng W D, Zhang G P. Positional variations in phytic acid and protein content within a panicle of japonica rice[J]. Journal of Cereal Science, 2005, 41(3): 297-303. |
| [26] | 韩展誉, 管弦悦, 赵倩, 吴春艳, 黄福灯, 潘刚, 程方民. 灌浆温度和氮肥及其互作效应对稻米贮藏蛋白组分的影响[J]. 作物学报, 2020, 46(7): 1087-1098. |
| [26] | Han Z Y, Guan X Y, Zhao Q, Wu C Y, Huang F D, Pan G, Cheng F M. Individual and combined effects of air temperature at filling stage and nitrogen application on storage protein accumulation and its different components in rice grains[J]. Acta Agromomica Sinica, 2020, 46(7): 1087-1098. (in Chinese with English abstract) |
| [27] | 莫惠栋. 农业试验统计[M]. 上海: 上海科学技术出版社, 1992: 151-166. |
| [27] | Mo H D. Agricultural Experiment Statistics[M]. Shanghai: Shanghai Science and Technology Press, 1992: 151-166. (in Chinese) |
| [28] | 胡培松. 功能性稻米研究与开发[J]. 中国稻米, 2003, 9(5): 3-5. |
| [28] | Hu P S. Research and exploitation of functional rice[J]. China Rice, 2003, 9(5): 3-5. (in Chinese) |
| [29] | 胡时开, 胡培松. 功能稻米研究现状与进展[J]. 中国水稻科学, 2021, 35(4):311-325. |
| [29] | Hu S K, Hu P S. Research progress and prospect of functional rice[J]. Chinese Journal Rice Science, 2021, 35(4): 311-325. (in Chinese with English abstract) |
| [30] | 苏宁, 万向元, 翟虎渠, 万建民. 功能型水稻研究现状和发展趋向[J]. 中国农业科学, 2007, 40(3): 433-439. |
| [30] | Su N, Wan X Y, Zhai H Q, Wan J M. Progress and prospect of functional rice researches[J]. Scientia Agricultura Sinica, 2007, 40(3): 433-439. (in Chinese with English abstract) |
| [31] | Mochizuki T, Hara S. Usefulness of low protein rice in diet therapy in patients with chronic renal failure[J]. Japanese Journal of Nephrology, 2000, 42(1): 24-29. (In Japanese with English abstract) |
| [32] | 王梨名, 刘金凤, 陈佳, 罗佳, 汪晓月, 何娅妮, 蔡明玉. 低谷蛋白大米(W0868)对小鼠营养状况及肾功能的影响[J]. 第三军医大学学报, 2021, 43(1):68-74 |
| [32] | Wang L M, Liu J F, Chen J, Luo J, Wang X Y, He Y N, Cai M Y. Effect of low-gluten rice (W0868) feeding on nutritional status and renal function in mice[J]. Journal of Third Military Medical University, 2021, 43(1): 68-74. (in Chinese with English abstract) |
| [33] | 胡国奥, 詹晓北, 李志涛, 朱莉, 赵志超, 张洪涛. 低谷蛋白大米在仿生大肠反应器中对肠道菌群结构及代谢的影响[J]. 食品与发酵工业, 2021, 47(13):23-29. |
| [33] | Hu G A, Zhan X B, Li Z T, Zhu L, Zhao Z C, Zhang H T. Effect of flow glutelin rice on composition and metabolism of intestinal flora in bionic large intestinal reactor[J]. Food and Fermentation Industries, 2021, 47(13): 23-29. (in Chinese with English abstract) |
| [34] | 张光恒, 曾大力, 郭龙彪, 刘慧娟, 胡江, 高振宇, 华志华, 钱前. 葡萄糖焦磷酸酶基因与巨胚基因聚合创建营养功能稻[J]. 中国水稻科学, 2007, 21(6):567-572. |
| [34] | Zhang G H, Zeng D L, Guo L B, Liu H J, Hu J, Gao Z Y, Hua Z H, Qian Q. Nutrition-functional rice created by polymerizing ADP-glucose pyrophosphorylase(AGP) and giant embryo (ge) genes[J]. Chinese Journal Rice Science, 2007, 21(6): 567-572. (in Chinese with English abstract) |
| [35] | Morita R, Kusaba M, Iida S, Nishio T, Nishimura M. Development of PCR markers to detect the glb1 and Lgc1 mutations for the production of low easy-to-digest protein rice varieties[J]. Theoretical and Applied Genetics, 2009, 119(1): 125-130. |
| [36] | 王萌, 李建粤. 分子标记辅助选育红米巨胚水稻[J]. 上海师范大学学报: 自然科学版, 2017, 46(5):647-653. |
| [36] | Wang M, Li J Y. Development of red giant embryo rice by molecular marker-assisted selection[J]. Journal of Shanghai Normal University (Natural Sciences), 2017, 46(5): 647-653. (in Chinese with English abstract) |
/
| 〈 |
|
〉 |