Chinese Journal OF Rice Science >
Effect of Rhizosphere Oxygen Environment on the Root Micromorphological Structure of Rice Seedlings and Its Physiological Mechanism
Received date: 2021-11-02
Revised date: 2021-12-29
Online published: 2022-07-12
【Objective】This study was conducted to research the effect of rhizosphere oxygen environment on the root micromorphological structure of rice seedlings and its physiological mechanism, so as to lay a theoretical basis for the construction of ideal root type of rice.【Method】Two varieties, Chunyou 84 and Xiushui 09, were cultured in nutrient solution with different oxygen content (OC) levels (low oxygen, OC 0－1.0 mg/L; medium oxygen, OC 2.5－3.5 mg/L; high oxygen, OC >6.0 mg/L, continuous bubbling with atmospheric air, and normal conditions as CK, conventional hydroponics without oxygen regulation, the oxygen content in water approximately 0.3－2.5 mg/L) controlled by on-line dissolved oxygen meters. After different oxygen treatments, the biomass, root micromorphological structure, and some root physiological indexes (organic acid content, respiratory intensity, and so on) of rice seedlings were measured and analyzed.【Result】1) The proportions of fine roots (d1, 0－0.50 mm; d2, 0.51－1.00 mm; d3, 1.01－1.50 mm) were more than 90%, which were particularly important for the construction of root morphology for rice seedlings. At medium oxygen level, the root length and root surface area of Chunyou 84 with diameter d1 and d2, and Xiushui 09 with diameter d1 and d3 were significantly higher than those of the control. 2) Low oxygen treatment reduced the biomass of shoot and root, inhibited the growth of fine roots (d1), promoted the growth of coarse roots (d5, 2.01－2.50 mm), reduced the root respiration rate and increased the secretion of organic acids. Medium and high oxygen treatments both increased the biomass of rice plants, but had different effects on root physiology. Medium oxygen treatment increased root respiration rate, while high oxygen treatment decreased. Different oxygen treatments reduced the content of root auxin (IAA). Especially, there was obvious difference between medium and high oxygen treatments with the control. The content of nitric oxide (NO) increased significantly under the high oxygen treatment, but there was no significant difference among other treatments. 3) The results of correlation analysis showed that the morphological indexes (root length, surface area, and volume) of the total and fine roots (d2) of the two varieties were positively correlated with root organic acid secretion, respiratory intensity, and nitric oxide content, on the contrary, they were negatively correlated with auxin content.【Conclusion】Moderate increase of dissolved oxygen concentration (medium oxygen treatment) could improve the root respiration rate and organic acid secretion of rice seedlings, enhancing the root function and increasing the proportion of the length and absorption area of fine root (d1－d3), optimizing the root morphological structure. Therefore, appropriate cultivation measures could change the rhizosphere oxygen environment, regulate the micro morphological structure of rice roots at tillering stage and enhance the function of roots in field.
XIAO Deshun, XU Chunmei, WANG Danying, ZHANG Xiufu, CHEN Song, CHU Guang, LIU Yuanhui . Effect of Rhizosphere Oxygen Environment on the Root Micromorphological Structure of Rice Seedlings and Its Physiological Mechanism[J]. Chinese Journal OF Rice Science, 2022 , 36(4) : 399 -409 . DOI: 10.16819/j.1001-7216.2022.211103
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