Chinese Journal OF Rice Science >
A Rapid Detection Assay of Nilaparvata lugens Based on Recombinase Aided Amplification-lateral Flow Dipstick Technology
Received date: 2021-03-29
Revised date: 2021-05-18
Online published: 2022-01-10
【Objective】To solve the time- and labor-costing problem for accurate identification of Nilarpavata lugens trapped by light, we aim to establish a fast and convenient qualitative identification method.【Method】At first, we selected a couple of primers which are species specific for N. lugens. Then, we established an identification method based on Rapid Aided Amplification-Lateral Flow Dipstick (RAA-LFD) technology. Finally, we analyzed the temperature and template robustness of this method. 【Result】The RAA-LFD method developed in the present study is convenient and fast, and it does not depend on any sophisticated instrument. Furthermore, this method can amplify effectively with crude tissue fluid as template, and it can be triggered by body temperature. The entire process for 56 specimens, including the crude tissue liquid preparation, isothermal amplification, and LFD detection, can be completed in 45 min with an accuracy of 100%. 【Conclusion】This RAA-LFD method is a time-saving and easy-to-apply molecular diagnostic method for N.lugens identification from its two sibling species. It is really suitable for Point of Care Test (POCT) in plant protection station or in field.
LUO Ju, TANG Jian, WANG Aiying, YANG Baojun, LIU Shuhua . A Rapid Detection Assay of Nilaparvata lugens Based on Recombinase Aided Amplification-lateral Flow Dipstick Technology[J]. Chinese Journal OF Rice Science, 2022 , 36(1) : 96 -104 . DOI: 10.16819/j.1001-7216.2022.210314
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