Chinese Journal OF Rice Science >
Research on the Mechanisim of OsPT4 Regulating the Accumulation and Utilization of Nitrogen and Phosphorus in Rice
#These authors contributed equally to this work
Received date: 2020-08-03
Revised date: 2020-12-04
Online published: 2021-11-10
【Objective】 The phosphate (Pi) transporter, OsPT4, is a member of the Pht1 family of rice, responsible for Pi uptake and transportation. It is important to explore the effect of OsPT4 overexpression on nitrogen (N) and phosphorous (P) accumulation and utilization in rice under different Pi conditions and its mechanism. 【Method】The OsPT4-overexpressing lines with Nipponbare background were used as material. Through the hydroponic and pot-cultivated experiments with (+Pi) or without Pi (-Pi) supply, the relative expression of OsPT4 in different tissues during reproductive growth stage was detected. The N and P concentrations in different tissues (leaf blade, leaf sheath, stem, rice hull, panicle axis and brown rice) under different Pi treatments were also measured. In addition, the Pi absorption rate and N/P use efficiency were calculated, and the plant height, yield per plant, 1000-grain weight and seed setting rate were analyzed. 【Result】The relative expression of OsPT4 was higher in rice roots during reproductive growth stage. Overexpression of OsPT4 increased the total P concentration in rice flag leaf blade, leaf sheaths, stems, rice hull, panicle axis and brown rice to varying degrees, and significantly improved the Pi absorption and use efficiency under different Pi regimes. Meanwhile, OsPT4 overexpression could significantly increase the yield per plant, 1000-grain weight and seed setting rate in +Pi and -Pi soils. In addition, the overexpression of OsPT4 also increased the total N concentration in unfilled rice hull and brown rice by 16.8% and 19.8%, and the N use efficiency by 6.6% under Pi-deficiency. 【Conclusion】The overexpression of OsPT4 not only promotes the Pi uptake and use efficiency, but also facilitates the physiology N use efficiency in different Pi conditions.
Key words： phosphorus; nitrogen; OsPT4; phosphorus use efficiency; nitrogen use efficiency
Yafei SUN, Ke SONG, Qin QIN, Lijuan SUN, Yong XUE . Research on the Mechanisim of OsPT4 Regulating the Accumulation and Utilization of Nitrogen and Phosphorus in Rice[J]. Chinese Journal OF Rice Science, 2021 , 35(6) : 565 -572 . DOI: 10.16819/j.1001-7216.2021.200803
| [1] | Mimura T.Regulation of phosphate transport and homeostasis in the plant cells[J]. International Review of Cytology, 1999, 191: 149-200. |
| [2] | Vance C P, Uhde-Stone C, Allan D L.Phosphorus acquisition and use: Critical adaptations by plants for securing a nonrenewable resource[J]. New Phytologist, 2003, 157: 423-447. |
| [3] | Schachtman D P, Reid R J, Ayling S M.Phosphorus uptake by plants: From soil to cell[J]. Plant Physiology, 1998, 116: 447-453. |
| [4] | Raghothama K G.Phosphate acquisition[J]. Annual Review of Plant Physiology and Plant Molecular Biology, 1999, 150: 665-693. |
| [5] | 朱德峰, 张玉屏, 陈惠哲, 向镜, 张义凯. 中国水稻高产栽培技术创新与实践. 中国农业科学, 2015, 48: 3404-3414. |
| [5] | Zhu D, Zhang Y, Chen H, Xiang J, Zhang Y K.Innovation and practice of high-yield rice cultivation technology in China.Scientia Agricultura Sinica, 2015, 48: 3404-3414. (in Chinese with English abstract). |
| [6] | Smith F W, Mudge S R, Rae A L, Donna G.Phosphate transporter in plants[J]. Plant and Soil, 2003, 248: 71-83. |
| [7] | Gu M, Chen A, Sun S, Xu G.Complex regulation of plant phosphate transporters and the gap between molecular mechanisms and practical application: What is missing?[J] Molecular Plant, 2016, 9: 396-416. |
| [8] | Poirier Y, Bucher M.Phosphate transport and homeostasis in Arabidopsis[J]. The Arabidopsis Book, 2002, (1): e0024. |
| [9] | Remy E, Cabrito T R, Batista R A, Teixeira M C, Sá-Correia I, Duquel P.The Pht1; 9 and Pht1;8 transporters mediate inorganic phosphate acquisition by the Arabidopsis thaliana root during phosphorus starvation[J]. New Phytologist, 2012, 195: 356-371. |
| [10] | Paszkowski U, Kroken S, Roux C, Briggs S P.Rice phosphate transporters include an evolutionarily divergent gene specifically activated in arbuscular mycorrhizal symbiosis[J]. Proceedings of the National Academy of Sciences of the United States of America, 2002, 99: 13324-13329. |
| [11] | Sun S, Gu M, Cao Y, Huang X, Zhang X, Ai P, Zhao J, Fan X, Xu G.A constitutive expressed phosphate transporter, OsPht1;1, modulates phosphate uptake and translocation in phosphate-replete rice[J]. Plant Physiology, 2012, 159: 1571-1581. |
| [12] | Ai P, Sun S, Zhao J, Fan X, Xin W, Guo Q, Yu L, Shen Q, Wu P, Miller A J, Xu G.Two rice phosphate transporters, OsPht1;2 and OsPht1;6, have different functions and kinetic properties in uptake and translocation[J]. The Plant Journal, 2009, 57: 798-809. |
| [13] | Zhang F, Wu X, Zhou H, Wang D, Jiang T, Sun Y, Cao Y, Pei W, Sun S, Xu G.Overexpression of rice phosphate transporter gene OsPT6 enhances phosphate uptake and accumulation in transgenic rice plants[J]. Plant and Soil, 2014, 384: 259-270. |
| [14] | Liu F, Wang Z, Ren H, Shen C, Li Y, Ling H-Q, Wu C, Lian X, Wu P.OsSPX1 suppresses the function of OsPHR2 in the regulation of expression of OsPT2 and phosphate homeostasis in shoots of rice[J]. The Plant Journal, 2010, 62: 508-517. |
| [15] | Chang M X, Gu M, Xia Y W, Dai X L, Dai C R, Zhang J, Wang S C, Qu H Y, Yamaji N, Ma J F, Xu G H.OsPHT1;3 mediates uptake, translocation and remobilization of phosphate under extremely low phosphate regimes[J]. Plant Physiology, 2019, 179: 656-670. |
| [16] | Zhang F, Sun Y, Pei W, Jain A, Sun R, Cao Y, Wu X, Jiang T, Zhang L, Fan X, Chen A, Shen Q, Xu G, Sun S.Involvement of OsPht1;4 in phosphate acquisition and mobilization facilitates embryo development in rice[J]. The Plant Journal, 2015: 82, 556-569. |
| [17] | Jia H F, Ren H Y, Gu M, Zhao J, Sun S, Zhang X, Chen J, Wu P, Xu G.The phosphate transporter gene OsPht1;8 is involved in phosphate homeostasis in rice[J]. Plant Physiology, 2011, 156: 1164-1175. |
| [18] | Wang Y F, Pineros M A, Wang Z Y, Wang W X, Wang W, Li C, Wu Z, Kochian L V, Wu P.Phosphate transporters OsPHT1;9 and OsPHT1;10 are involved in phosphate uptake in rice[J]. Plant Cell and Environment, 2014, 37: 1159-1170. |
| [19] | Marschner H.Mineral Nutrition of Higher Plants[M]. London: Academic Press, 1995. |
| [20] | Peng M, Hannam C, Gu H, Bi Y M, Rothstein S J.A mutation in NLA, which encodes a RING-type ubiquitin ligase, disrupts the adaptability of Arabidopsis to nitrogen limitation[J]. The Plant Journal, 2007, 50: 320-337. |
| [21] | Kant S, Peng M, Rothstein S J.Genetic regulation by NLA and microRNA827 for maintaining nitrate dependent phosphate homeostasis in Arabidopsis[J]. PLoS Genetics, 2011, 7: e1002021. |
| [22] | Lin W Y, Huang T K, Chiou T J.NITROGEN LIMITATION ADAPTATION, a target of MicroRNA827, mediates degradation of plasma membrane-localized phosphate transporters to maintain phosphate homeostasis in Arabidopsis[J]. Plant Cell, 2013, 25: 4061-4074. |
| [23] | Medici A, Marshall-Colon A, Ronzier E, Szponarski W, Wang R, Gojon A, Crawford N M, Ruffel S, Coruzzi G M, Krouk G.AtNIGT1/HRS1 integrates nitrate and phosphate signals at the Arabidopsis root tip[J]. Nature Communication, 2015, 6: 6274. |
| [24] | Kiba T, Inaba J, Kudo T, Ueda N, Konishi M, Mitsuda N, Takiguchi Y, Kondou Y, Yoshizumi T, Ohme-Takagi M, Matsui M, Yano K, Yanagisawa S, Sakakibara H.Repression of nitrogen starvation responses by members of the Arabidopsis GARP-type transcription factor NIGT1/HRS1 subfamily[J]. Plant Cell, 2018, 30: 925-945. |
| [25] | Maeda Y, Konishi M, Kiba T, Sakuraba Y, Sawaki N, Kurai T, Ueda Y, Sakakibara H, Yanagisawa S.A NIGT1-centred transcriptional cascade regulates nitrate signaling and incorporates phosphorus starvation signals in Arabidopsis[J]. Nature Communications, 2018, 9: 1376. |
| [26] | Medici A, Szponarsk W, Dangeville P, Safi A, Dissanayake I M, Saenchai C, Emanuel A, Rubio V, Lacombe B, Ruffel S, Tanurdzic M, Rouached H, Krouk G.Nitrogen actively controls the phosphate starvation response in plants[J]. Plant Cell, 2019, 31: 1171-1184. |
| [27] | Lv Q, Zhong Y, Wang Y, Wang Z, Zhang L, Shi J, Wu Z, Liu Y, Mao C, Yi K, Wu P.SPX4 negatively regulates phosphate signaling and homeostasis through its interaction with PHR2 in rice[J]. Plant Cell, 2014, 26: 1586-1597. |
| [28] | Hu B, Jiang Z, Wang W, Qiu Y, Zhang Z, Liu Y, Li A, Gao X, Liu L, Qian Y, Huang X, Yu F, Kang S, Wang Y, Xie J, Cao S, Zhang L, Wang Y, Xie Q, Kopriva S, Chu C. Nitrate-NRT1.1B-SPX4 cascade integrates nitrogen and phosphorus signaling networks in plants[J]. Nature Plants, 2019, 5: 401-413. |
| [29] | 张占田, 孙雅菲, 艾昊, 罗闻真, 冯冰, 孙文献, 徐国华, 孙淑斌. 水稻转录因子基因OsSHR2的时空表达特征及其在营养生长中的调控[J]. 中国水稻科学, 2018, 32: 427-436. |
| [29] | Zhang Z T, Sun Y F, Ai H, Luo W Z, Feng B, Sun W X, Xu G H, Sun S B.Expression patterns and regulation of transcription factor gene OsSHR2 in vegetative growth in rice[J]. Chinese Journal of Rice Science, 2018, 32: 427-436. (in Chinese with English abstract). |
| [30] | Livak K J, Schmittgen T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔCT method[J]. Methods, 2001, 25: 402-408. |
| [31] | Elliott G C, Lauchli A.Phosphorus efficiency and phosphate iron interactions in maize[J]. Agronomy Journal, 1985, 77: 399-403. |
| [32] | Siddiqi M Y, Glass A D M. Utilization index: A modified approach to the estimation and comparison of nutrient utilization efficiency in plants[J]. Journal of Plant Nutrition, 1981, 4: 289-302. |
| [33] | Fan X, Tang Z, Tan Y, Zhang Y, Luo B, Yang M, Lian X, Shen Q, Miller A J, Xu G.Overexpression of a pH-sensitive nitrate transporter in rice increases crop yields[J]. Proceedings of the National Academy of Sciences of the United States of America, 2016, 113: 7118-7123. |
| [34] | Lynch J P.Root phenes for enhanced soil exploration and phosphorus acquisition: Tools for future crops[J]. Plant Physiology, 2007, 156: 1041-1049. |
| [35] | Ye Y, Yuan J, Chang X, Yang M, Zhang L.The phosphate transporter gene OsPht1; 4 is involved in phosphate homeostasis in rice[J]. PLoS ONE, 2015, 10: e0126186. |
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