Chinese Journal OF Rice Science >
Transcriptome Analysis of Hormone Signal Transduction and Glutathione Metabolic Pathway in Rice Seeds at Germination Stage
Received date: 2020-09-23
Revised date: 2021-03-04
Online published: 2021-11-10
【Objective】By using transcriptome sequencing technology, we explored the regulation mechanism of hormone signal transduction and redox balance inside cells during rice germination, as a way to increase the understanding of and construct the complex regulatory network of germination. At the same time, mining the genes that regulate seed germination could lay a theoretical basis for direct-seeded rice breeding.【Method】Dynamic transcriptome sequencing analysis was performed using seeds at 0, 24, and 48 hours after imbibition as materials. Differentially expressed genes (DEGs) were screened with the threshold of Fold Change≥2 and False Discovery Rate≤ 0.05. Gene Ontology and KEGG Pathway databases were used to analyze and annotate the DEGs at different stages of germination and real-time quantitative PCR was conducted to verify the sequencing results. Finally, the protein interaction network of DEGs was analyzed using the String protein interaction database under the threshold of combined_score≥0.9.【Result】8719 DEGs were identified in the early stage of seed germination, but only 3480 DEGs in the late stage of germination. The results of GO and KEGG enrichment analysis showed that the DEGs related to hormone signal transduction were mainly induced in the early stage, especially the GH3 (Gretchen Hagen 3) family genes in the auxin signal transduction pathway were all significantly induced after imbibition. While the DEGs involved in the glutathione metabolism pathway were more active during the late germination period, and the glutathione-S-transferase genes were the most enriched in this pathway. In addition, two isocitrate dehydrogenase (ICDH) genes were significantly induced throughout the germination process. According to protein interaction prediction, the two ICDH genes may interact with the GH3 family genes.【Conclusion】The GH3 family genes in the AUX signal transduction pathway may play an important role in attenuating the AUX signal and reducing the AUX activity in the early stage of seed germination. Their high expression levels reduce the regulatory effect of auxin on seed dormancy and promote seed germination. In the glutathione metabolism pathway, GST genes may play a major role in resistance to cell oxidative stress in the late stage of seed germination. In addition, the interaction between GH3 and ICDH family genes during the germination process may have important significance in realizing the tandem effect of hormone transduction pathway and glutathione metabolism pathway and co-regulating seed germination.
Key words： rice; germination; transcriptome; hormone; glutathione
Huan CUI, Qiaoli GAO, Lixin LUO, Jing YANG, Chun CHEN, Tao GUO, Yongzhu LIU, Yongxiang HUANG, Hui WANG, Zhiqiang CHEN, Wuming XIAO . Transcriptome Analysis of Hormone Signal Transduction and Glutathione Metabolic Pathway in Rice Seeds at Germination Stage[J]. Chinese Journal OF Rice Science, 2021 , 35(6) : 554 -564 . DOI: 10.16819/j.1001-7216.2021.200915
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