Chinese Journal OF Rice Science >
Distribution of Grain Shape Related Genes in Rice Big Grain Germplasm BG1 and Elite Restorer Line Huazhan and Development of Relevant Functional Markers
Received date: 2020-12-15
Revised date: 2021-02-09
Online published: 2021-11-10
【Objective】The exploration and utilization of specific germplasm resources are one of the important means of rice breeding with high quality and high yield. Identifying the allelic variation types of some grain shape genes carried by Big Grain 1 (BG1) and Huazhan, a high quality restorer line widely used in rice breeding, and developing functional markers of corresponding genes are helpful to speed up the application of grain shape genes in rice breeding and improve the efficiency of accurate improvement of grain shape in rice.【Method】The target fragments of nine major grain shape genes (GS3, qLGY3, qGL3, GW2, GW8, GW5, TGW3, TGW6, GS9) in BG1 and Huazhan were sequenced and analyzed. According to the sequencing results of GW2, GS3, qGL3, TGW3, TGW6, qLGY3, GW8 and GW5, a functional marker for genotyping was developed.【Result】Compared with Nipponbare, 5 of the 9 major grain shape genes detected in BG1 showed functional deletion (gs3, GW8, gw2, tgw3, tgw6), 1 rare allele variation (qgl3) and 1 exon variable shear (qlgy3); Compared with Nipponbare, Huazhan had differences in 3 of the 9 major grain shape genes (gs3, GW8 and GW5). According to the sequencing results, the functional markers of GW2, GS3, QGL3, TGW3, TGW6, QLGY3, GW8 and GW5 were developed and 15 rice germplasm were used for detection. In the detection results, in addition to the marker tgw6-del of TGW6, which can only be used to identify whether there is allelic variation tgw6 of functional deletion type, the other 7 markers can accurately identify genotype. There was sequence variation of GS9 among the tested materials, which did not affect the change of grain shape. According to the above sequencing results, the functional markers of GW2, GS3, qGL3, TGW3, TGW6, qLGY3, GW8 and GW5 were developed and detected by 15 rice germplasms. Except for the marker TGW6-Del for TGW6, which can only be used to identify the allelic variant tgw6, a functional deletion type, the other seven markers can be genotyped accurately.【Conclusion】Compared with Nipponbare, the genes gs3, qgl3, qlgy3, tgw3, GW8 and tgw6 that regulate grain length in extra-large grain germplasm BG1 may have complex interaction pathways rather than simple effect accumulation, and the grain width may be the cumulative effect of gw2 and GW8. The slender grain shape of high quality restorer line Huazhan may be caused by the interaction effect of gs3, GW5 and GW8. The functional markers developed can be used in rice molecular marker-assisted breeding.
Key words： grain shape; grain length; grain width; grain weight; functional marker
Ke GONG, Pao XUE, Xiaoxia WEN, Feifei LIAO, Bin SUN, Zequn PENG, Shihua CHENG, Liyong CAO, Yingxin ZHANG, Weixun WU, Lianping SUN, Xiaodeng ZHAN . Distribution of Grain Shape Related Genes in Rice Big Grain Germplasm BG1 and Elite Restorer Line Huazhan and Development of Relevant Functional Markers[J]. Chinese Journal OF Rice Science, 2021 , 35(6) : 543 -553 . DOI: 10.16819/j.1001-7216.2021.201211
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