Chinese Journal OF Rice Science >
Cloning and Functional Analysis of Rice Tillering Regulatory Gene HTD3
#These authors contributed equally to this work
Received date: 2021-02-16
Revised date: 2021-03-23
Online published: 2021-11-10
【Objective】Cloning of rice tiller-related genes lays a theoretical basis and provides beneficial genetic resources for constructing ideal plant-type rice and increasing grain yield.【Method】We compared phenotypes and main agronomic traits between the mutant htd3 (high-tillering dwarf 3) and its wild type under conventional field planting conditions, used map-based cloning method to clone candidate genes, analyzed the expression levels of HTD3, SL and ABA-related genes by fluorescence quantitative real-time PCR. Sequencing comparison was performed to analyze the natural variation of HTD3 in 147 germplasm resources.【Result】Compared with the wild type, the axillay bud of the mutant htd3 grow faster, the number of tillers and effective panicles were significantly increased, the plant height, the number of primary rachis branches and grains per panicle were significantly decreased, and the seed setting rate and 1000-grain weight were not significantly changed. Genetic analysis indicated that the high-tillering trait of htd3 was controlled by single recessive nuclear gene, which was mapped to a 63.5 kb region between the marker CM8 and CM10 on chromosome 12, and the complementary transgenic experiment proved that LOC_Os12g21710 was the gene controlling the high-tillering phenotype. HTD3 is constitutively expressed in wild-type and mutant, and the mutation of this gene could up-regulate the expression level of some stratolactones and ABA-related genes. The natural variation of G2674A in the HTD3 coding region in rice varieties significantly increase the number of tillers.【Conclusion】HTD3 is a new allele of the recently reported T20/MIT1 gene. The HTD3 mutation leads to the phenotype of moderate increase in tillers and slightly shorter plant height in rice, which has great application potential in cultivating ideal plant type rice and high-yield breeding.
Key words： rice; high-tillering; gene cloning; biological function
Xianmei WU, Sanfeng LI, Ping HU, Rui HE, Ran JIAO, Yijian MAO, Caolin LU, Juan HU, Han LIN, Rongliang WU, Xudong ZHU, Yuchun RAO, Yuexing WANG . Cloning and Functional Analysis of Rice Tillering Regulatory Gene HTD3[J]. Chinese Journal OF Rice Science, 2021 , 35(6) : 535 -542 . DOI: 10.16819/j.1001-7216.2021. 210205
| [1] | 李学勇, 钱前, 李家洋. 水稻分蘖的分子机理研究[J]. 中国科学院院刊, 2003, 18(4): 274-276. |
| [1] | Li X Y, Qian Q, Li J Y.Progress in elucidating the molecular mechanism of rice tillering[J]. Bulletin of the Chinese Academy of Sciences, 2003, 18(4): 274-276. (in Chinese with English abstract) |
| [2] | Wu T, Shen Y, Zheng M, Yang C, Chen Y, Feng Z, Liu X, Liu S, Chen Z, Lei C, Wang J, Jiang L, Wan J.Gene SGL, encoding a kinesin-like protein with transactivation activity, is involved in grain length and plant height in rice[J]. Plant Cell Reports, 2014, 33(2): 235-244. |
| [3] | Li X Y, Qian Q, Fu Z M, Wang Y H, Xiong G S, Zeng D L, Wang X Q, Liu X F, Teng S, Hiroshi F, Yuan M, Luo D, Han B, Li J Y.Control of tillering in rice[J]. Nature, 2003, 422(6932): 618-621. |
| [4] | Xu C, Wang Y, Yu Y, Duan J, Liao Z, Xiong G, Meng X, Liu G, Qian Q, Li J.Degradation of MONOCULM 1 by APC/C(TAD1) regulates rice tillering[J]. Nature Communications, 2012, 3(1): 1-9. |
| [5] | Liang W H, Shang F, Lin Q T, Lou C, Zhang J.Tillering and panicle branching genes in rice[J]. Gene, 2014, 537(1): 1-5. |
| [6] | Kim H, Hwang H, Hong J W, Lee Y N, Ahn I P, Yoon I S, Yoo S D, Lee S, Lee S C, Kim B G.A rice orthologue of the ABA receptor, OsPYL/RCAR5, is a positive regulator of the ABA signal transduction pathway in seed germination and early seedling growth[J]. Journal of Experimental Botany, 2012, 63(2): 1013-1024. |
| [7] | Ljung K, Bhalerao R P, Sandberg G.Sites and homeostatic control of auxin biosynthesis in Arabidopsis during vegetative growth. Plant J, 2001, 28(4):465-474. |
| [8] | Lee M, Jung J H, Han D Y, Seo P J, Park W J, Park C M.Activation of a flavin monooxygenase gene YUCCA7 enhances drought resistance in Arabidopsis[J]. Planta, 2012, 235(5): 923-938. |
| [9] | Xu M, Zhu L, Shou H, Wu P.A PIN1 family gene, OsPIN1, involved in auxin-dependent adventitious root emergence and tillering in rice[J]. Plant Cell Physiology, 2005, 46(10): 1674-1681. |
| [10] | Xu J X, Ding C Q, Ding Y F, Tang S, Zha M R, Luo B J, Wang S H.A proteomic approach to analyze differential regulation of proteins during bud outgrowth under apical dominance based on the auxin transport canalization model in rice (Oryza sativa L.)[J]. Journal of Plant Growth Regulation, 2015, 34(1): 122-136. |
| [11] | Lin H, Wang R, Qian Q, Yan M, Meng X, Fu Z, Yan C, Jiang B, Su Z, Li J, Wang Y.DWARF27, an iron-containing protein required for the biosynthesis of strigolactones, regulates rice tiller bud outgrowth[J]. Plant Cell, 2009, 21(5): 1512-1525. |
| [12] | Zou J, Zhang S, Zhang W, Li G, Chen Z, Zhai W, Zhao X, Pan X, Xie Q, Zhu L.The rice HIGH-TILLERING DWARF1 encoding an ortholog of Arabidopsis MAX3 is required for negative regulation of the outgrowth of axillary buds[J]. Plant Journal, 2006, 48(5): 687-698. |
| [13] | Arite T, Iwata H, Ohshima K, Maekawa M, Nakajima M, Kojima M, Sakakibara H, Kyozuka J.DWARF10, an RMS1/MAX4/DAD1 ortholog, controls lateral bud outgrowth in rice[J]. Plant Journal, 2007, 51(6): 1019-1029. |
| [14] | Fang Z, Ji Y, Hu J, Guo R, Sun S, Wang X.Strigolactones and brassinosteroids antagonistically regulate the stability of the D53-OsBZR1 complex codetermine FC1 expression in rice tillering[J]. Molecular Plant, 2020, 13(4): 586-597. |
| [15] | Zhao J, Wang T, Wang M, Zhao J, Wang T, Wang M, Liu Y, Yuan S, Gao Y, Yin L, Sun W, Wan J, Li X.DWARF3 participates in an SCF complex and associates with DWARF14 to suppress rice shoot branching[J]. Plant Cell Physiology, 2014, 55(6): 1096-1109. |
| [16] | Arite T, Umehara M, Ishikawa S, Hanada A, Maekawa M, Yamaguchi S, Kyozuka J.d14, a strigolactone-insensitive mutant of rice, shows an accelerated outgrowth of tillers[J]. Plant Cell Physiology, 2009, 50(8): 1416-1424. |
| [17] | Liu W, Wu C, Fu Y, Hu G, Si H, Zhu L, Luan W, He Z, Sun Z.Identification and characterization of HTD2: A novel gene negatively regulating tiller bud outgrowth in rice[J]. Planta, 2009, 230(4): 649-658. |
| [18] | Gao Z, Qian Q, Liu X, Yan M, Feng Q, Dong G, Liu J, Han B.Dwarf 88, a novel putative esterase gene affecting architecture of rice plant[J]. Plant Molecular Biology, 2009, 71(3): 265-276. |
| [19] | Ishikawa S, Maekawa M, Arite T, Onishi K, Takamure I, Kyozuka J.Suppression of tiller bud activity in tillering dwarf mutants of rice[J]. Plant Cell Physiology, 2005, 46(1): 79-86. |
| [20] | De S A, Clavé G, Badet-Denisot M A, Pillot J P, Cornu D, Le Caer J P, Burger M, Pelissier F, Retailleau P, Turnbull C, Bonhomme S, Chory J, Rameau C, Boyer F D. An histidine covalent receptor and butenolide complex mediates strigolactone perception[J]. Nature Chemical Biology, 2016, 12(10): 787-794. |
| [21] | Sharma R, De V D, Sharma M K, Ronald P C.Recent advances in dissecting stress-regulatory crosstalk in rice[J]. Molecular Plant, 2013, 6(2): 250-260. |
| [22] | Yao R, Ming Z, Yan L, Li S, Wang F, Ma S, Yu C, Yang M, Chen L, Li Y, Yan C, Miao D, Sun Z, Yan J, Sun Y, Wang L, Chu J, Fan S, He W, Deng H, Nan F, Li J, Rao Z, Lou Z, Xie D.DWARF14 is a non-canonical hormone receptor for strigolactone[J]. Nature, 2016, 536(7617): 469-473. |
| [23] | Zhou F, Lin Q, Zhu L, Ren Y, Zhou K, Shabek N, Wu F, Mao H, Dong W, Gan L, Ma W.D14-SCF(D3)- dependent degradation of D53 regulates strigolactone signaling[J]. Nature, 2013, 504(7480): 406-410. |
| [24] | Takeda T, Suwa Y, Suzuki M, Kitano H, Ueguchi- Tanaka M, Ashikari M, Matsuoka M, Ueguchi C.The OsTB1 gene negatively regulates lateral branching in rice[J]. Plant Journal, 2003, 33(3): 513-520. |
| [25] | Umehara M, Hanada A, Magome H, Takeda K N, Yamaguchi S.Contribution of strigolactones to the inhibition of tiller bud outgrowth under phosphate deficiency in rice[J]. Plant Cell Physiology, 2010, 51(7): 1118-1126. |
| [26] | Wang Y, Shang L, Yu H, Zeng L, Hu J, Ni S, Rao Y, Li S, Chu J, Meng X, Wang L, Hu P, Yan J, Kang S, Qu M, Lin H, Wang T, Wang Q, Hu X, Chen H, Wang B, Gao Z, Guo L, Xiong G, Li J, Qian Q.A strigolactone biosynthesis gene contributed to the green revolution in rice[J]. Molecular Plant, 2020, 13(6): 923-932. |
| [27] | Liu X, Hu Q, Yan J, Sun K, Liang Y, Jia M, Meng X, Fang S, Wang Y, Jing Y, Liu G, Wu D, Chu C, Smith S M, Chu J, Wang Y, Li J, Wang B.ζ-carotene isomerase suppresses tillering in rice through the coordinated biosynthesis of strigolactone and abscisic acid[J]. Molecular Plant, 2020, 13(12): 1784-1801. |
| [28] | Liu L, Ren M, Peng P, Chun Y, Li L, Zhao J, Fang J, Peng L, Yan J, Chu J, Wang Y, Yuan S, Li X.MIT1, encoding a 15-cis-ζ-carotene isomerase, regulates tiller number and stature in rice[J]. Journal of Genet Genomics, 2021, 48(1): 88-91. |
| [29] | 陈彩艳, 邹军煌, 张淑英, 朱立煌. 独角金内酯能抑制植物的分枝并介导植物与枞枝真菌及寄生植物间的相互作用[J]. 中国科学: 生命科学, 2009(6): 525-533. |
| [29] | Chen C, Zou J, Zhang S, Zhu L.Strigolactones are a new-defined class of plant hormones which inhibit shoot branching and mediate the interaction of plant-AM fungi and plant-parasitic weeds[J]. Science in China: Life Sciences, 2009(6): 525-533. (in Chinese with English abstract) |
| [30] | Wang P, Gao J, Wan C, Zhang F, Xu Z, Huang X, Sun X, Deng X.Divinyl chlorophyll(ide) a can be converted to monovinyl chlorophyll(ide) a by a divinyl reductase in rice[J]. Plant Physiology, 2010, 153(3): 994-1003. |
| [31] | Cline M G, Oh C.A reappraisal of the role of abscisic acid and its interaction with auxin in apical dominance[J]. Annual Botany, 2006, 98(4): 891-897. |
| [32] | 刘杨. 水稻分蘖芽萌发与休眠相互转换的激素学机制[D]. 南京: 南京农业大学, 2011. |
| [32] | Liu Y.The Mechanism of hormonal regulation of the transformation between germination and dormancy of rice tiller buds[D]. Nanjing: Nanjing Agricultural University.(in Chinese with English abstract) |
| [33] | Zang G, Zou H, Zhang Y.The De-Etiolated 1 homolog of Arabidopsis modulates the ABA signaling pathway and ABA biosynthesis in rice[J]. Plant Physiology, 2016, 171(2): 1259-1276. |
| [34] | Bang S W, Park S H, Jeong J S, Kim Y S, Jung H, Ha S H, Kim J K.Characterization of the stress-inducible OsNCED3 promoter in different transgenic rice organs and over three homozygous generations[J]. Planta, 2013, 237(1): 211-224. |
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